fibrin and Rectal-Neoplasms

Topics: Adult; Antineoplastic Agents; Blood Coagulation; Blood Coagulation Factors; Breast Neoplasms; Dextrans; Female; Fibrin; Fibrinogen; Fibrinolysis; Humans; Male; Middle Aged; Neoplasm Metastasis; Neoplasms; Ovarian Neoplasms; Rectal Neoplasms; Sulfates

Fibrinogen Matsumoto III (M-III) is a dysfibrinogen identified in a 66-year-old woman with rectal cancer. The fibrinogen level determined by the thrombin-time method was markedly decreased in preoperative coagulation tests of her plasma. Three fibrinogen polypeptide-chain gene fragments from the proposita were amplified by the polymerase chain reaction method, then sequenced. The triplet CGC encoding the amino acid residue gamma275 was replaced by TGC, resulting in the substitution of Arg->Cys. There have been previous reports of nine families with the same alteration, nine families with an Arg->His variant and one family with an Arg->Ser variant in this residue, which has been shown to be one of the most important amino acids in the 'D:D' interaction site. In addition, there are three silent mutations in the Aalpha-chain gene and two mutations in the intron of the Bbeta-chain and the gamma-chain gene. However, none of these mutations is thought to be the cause of the dysfunctional fibrinogen. The thrombin-catalyzed fibrin polymerization in the presence of 1 mM Ca ions was markedly delayed in purified M-III. Its lag period was longer than those of Matsumoto II (M-II; gamma308Asn->Lys) and Matsumoto I (M-I; gamma364Asp-His). gamma364Asp is one of the most important residues in the polymerization pocket of the 'D:E' interaction site and gamma308Asn is located in the vicinity of a high affinity Ca2+ binding site in the D-domain, gamma311-336. The maximum slope of the polymerization curve for M-III was about 4-fold steeper than that for M-1 but less steep than that for M-II. These results may suggest that the tertiary structure of the polymerization pocket plays a more important role in the lateral aggregation of protofibrils than that of the 'D:D' interaction site.

Topics: Aged; Dimerization; Female; Fibrin; Fibrinogens, Abnormal; Humans; Point Mutation; Rectal Neoplasms; Substrate Specificity

Topics: Adenocarcinoma; Adult; Central Nervous System Diseases; Fibrin; Humans; Lymphatic Metastasis; Male; Peripheral Nervous System Diseases; Rectal Neoplasms

Causal connection between shock and SACH has been suggested on the basis of clinical analysis. In a case of cardiogenic shock demonstrating criteria of initial SACH, successful identification of intravascular fibrinoid thrombi is presented as valid evidence for this pathogenetic presumption.

Topics: Aged; Cholecystitis; Fibrin; Gallbladder Diseases; Humans; Rectal Neoplasms; Shock, Cardiogenic; Thrombosis

Topics: Aged; Biopsy; Female; Fibrin; Fibrinogen; Fibrinolysis; Humans; Iodine Radioisotopes; Male; Middle Aged; Plasminogen; Postoperative Complications; Rectal Neoplasms; Thrombophlebitis; Veins

Topics: Breast Neoplasms; Colonic Neoplasms; Esophageal Neoplasms; Fibrin; Fibrinogen; Fibrinolysis; Gallbladder Neoplasms; Humans; Lymphatic Metastasis; Neoplasms; Pancreatic Neoplasms; Rectal Neoplasms; Stomach Neoplasms

Topics: Aminocaproates; Blood Coagulation; Breast Neoplasms; Colonic Neoplasms; Female; Fibrin; Fibrinogen; Fibrinolysis; Gastrointestinal Neoplasms; Humans; Immunoelectrophoresis; Intestinal Neoplasms; Kidney Neoplasms; Lung Neoplasms; Lymphatic Metastasis; Male; Neoplasm Metastasis; Neoplasm Recurrence, Local; Pancreatic Neoplasms; Prostatic Neoplasms; Rectal Neoplasms; Thrombin; Urinary Bladder Neoplasms; Urogenital Neoplasms

Topics: Aged; Ascites; Breast; Breast Neoplasms; Cell Division; Culture Techniques; Epithelium; Female; Fibrin; Humans; Middle Aged; Neoplasms; Nylons; Ovarian Neoplasms; Pleura; Rectal Neoplasms