fibrin and Psoriasis

Topics: Aging; Animals; Blood Vessels; Body Fluids; Capillary Permeability; Fibrin; Growth; Humans; Infant, Newborn; Lymphatic System; Microcirculation; Oxygen Consumption; Proteins; Psoriasis; Regional Blood Flow; Skin; Skin Temperature

Psoriasis is a chronic, immune-mediated inflammatory skin disease, affecting approximately 2% of the general population, which can be accompanied by psoriatic arthritis (PsA). The condition has been associated with an increased cardiovascular burden. Hypercoagulability is a potential underlying mechanism that may contribute to the increased risk of major cardiovascular events in psoriatic individuals. Whole blood samples were collected from 20 PsA patients and 20 healthy individuals. The concentrations of inflammatory molecules (C-reactive protein, serum amyloid A, soluble intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1, and soluble P-selectin) were determined by enzyme-linked immunosorbent assays. In addition, clotting efficiency was evaluated by thromboelastography. The fibrin network architecture was also assessed by scanning electron microscopy. Elevated levels of circulating inflammatory molecules were significantly associated with the presence of psoriatic disease. Furthermore, an increased tendency towards thrombus formation was significantly predictive of disease presence. Scanning electron microscopy revealed that fibrin clots were denser in psoriatic individuals, compared to healthy controls, with an increased fibrin fibre diameter associated with psoriatic disease. Our results add to the accumulating evidence of the systemic nature of psoriasis and the subsequent risk of cardiovascular comorbidities, potentially due to an acquired hypercoagulability. We suggest that haemostatic function should be monitored carefully in psoriatic patients that present with severe disease, due to the pre-eminent risk of developing thrombotic complications.

Topics: Arthritis, Psoriatic; Case-Control Studies; Endothelial Cells; Female; Fibrin; Hemostatics; Humans; Inflammation; Logistic Models; Male; Middle Aged; Psoriasis; Thrombelastography

Plasma from patients with active psoriasis has been shown to induce the formation of stellate fibrin-fibronectin microclots in vitro around cultured keratinocytes and fibroblasts. Such a stellate radiation of fibronectin and fibrin was also demonstrated around monocytes from patients with psoriasis. The phenomenon was not observed in healthy subjects but has been found in various disorders.

Topics: Adolescent; Adult; Aged; Cells, Cultured; Eczema; Epidermal Cells; Fibrin; Fibroblasts; Fibronectins; Fluorescent Antibody Technique; Humans; Leg Ulcer; Middle Aged; Psoriasis

The in vitro formation of fibrin microclots in blood from patients with psoriasis was studied before, during and after treatment. When a bacterial lipopolysaccharide was added to the blood, the microclot test was positive in 0% of controls, in 17% of the patients with untreated slight psoriasis and in 100% of those with severe psoriasis. A positive test without added lipopolysaccharide indicates the presence of circulating endotoxins. It was negative in all patients with slight psoriasis but positive in 75% of those with a severe form. During treatment the microclots decreased concurrently with clinical improvement in 79% of the patients. After treatment, eleven patients still had a positive test and nine of these patients showed a relapse within 1-2 months. Warfarin treatment rapidly inhibited microclot formation but this had little or no effect on the psoriatic lesions. High doses of potent corticosteroids under occlusion inhibited microclot formation for some hours. It seems likely that there may be a release of endotoxins in severe psoriasis which is decreased during successful treatment.

Topics: Adult; Aged; Blood Coagulation; Blood Coagulation Tests; Endotoxins; Fibrin; Humans; Lipopolysaccharides; Middle Aged; Psoriasis

Direct immunofluorescent microscopy was positive on skin biopsy specimens in thirty-one of sixty patients with various forms of psoriasis. Patients with positive immunofluorescence were classified into five main clinical subgroups: (1) psoriasis vulgaris with decreased tolerance to sunlight or ultraviolet light B (eight patients), (2) psoriatic arthritis (seven patients), (3) guttate psoriasis (five patients), (4) pustular psoriasis (four patients), and (5) "uncomplicated" psoriasis vulgaris (seven patients). Various patterns of deposition of immunoglobulins, complement, and fibrin were seen in the five subgroups, the most consistent being granular C3 at the basement membrane zone in specimens from five of the seven patients with guttate psoriasis. Direct immunofluorescent microscopy was usually negative in psoriasis vulgaris, but positive results may be anticipated in special forms of psoriasis.

Topics: Biopsy; Complement C3; Fibrin; Fluorescent Antibody Technique; Humans; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Psoriasis; Skin

Psoriatic arthritis (PA) is included in the seronegative arthritis group, though it is now generally considered to represent a clinical entity. In PA, in contrast to psoriasis vulgaris and to other types of rheumatoid arthritis, only a few immunological studies have been reported. In the present report synovial joint membranes from patients with PA and control groups have been studied for the presence of (a) vascular changes, (b) fibrin, (c) immunoglobulins and complement factor C3.

Topics: Arthritis; Arthritis, Rheumatoid; Complement C3; Fibrin; Fluorescent Antibody Technique; Humans; Immunoglobulins; Psoriasis; Synovial Membrane; Vasculitis

Radially-oriented fibrin crystallization was induced by incubation of blood from psoriatic patients with killed Pseudomonas aeruginosa. The phenomenon was inhibited by irradiation of the buffy coat in plasma with UV-B. It was not inhibited by UV-A unless trioxsalen had been added. Addition of UV-A-irradiated plasma to non-irradiated buffy coat also inhibits the fibrin formation.

Topics: Crystallization; Fibrin; Humans; Male; Psoriasis; Trioxsalen; Ultraviolet Rays

Radially oriented acicular crystalline aggregates could be induced by incubating heparinised blood with bacterial endotoxins. These aggregates did not appear in the blood of 37 healthy volunteers but were observed in the blood of 130 patients, predominantly those with vasculitis, psoriasis, and bacterial infections. Study of these asteroid structures, which resemble 'sunbursts', led to the view that they are oriented crystals of fibrin radiating from a central platelet mass undergoing lysis.

Topics: Bacterial Infections; Blood Platelets; Crystallization; Dermatitis; Endotoxins; Enterobacteriaceae; Fibrin; Hemolysis; Humans; Hypersensitivity; In Vitro Techniques; Pseudomonas aeruginosa; Psoriasis; Streptococcus; Vascular Diseases

Heparinized blood from patients with a variety of dermatological problems was incubated with killed bacteria, bacterial extracts or Gram-negative bacterial endotoxin. Within 18-24 h asteroid bodies consisting of an amorphous centre with fine radiating needle crystals were seen. These bodies, not found in the blood of healthy volunteers, were most consistently observed in blood from patients with psoriasis, vasculitis and bacterial infections. Immunofluorescent studies disclosed the asteroid formations to be crystals of fibrin radiating presumably from a central platelet aggregate. The failure to demonstrate IgG, A, M or D suggests that the phenomenon results from the bacterial activation of complement by a non-immune yet specific pathway which in turn involves white cells, platelet aggregation and release of procoagulants.

Topics: Antigens, Bacterial; Bacterial Toxins; Crystallization; Female; Fibrin; Humans; Hypersensitivity; Male; Polysaccharides, Bacterial; Psoriasis; Skin Diseases

Four cases are presented in which probable bullous pemphigoid and probable psoriasis co-existed. One case represents a simple coincidence of the two diseases. In the other three cases, the bullous lesions were transient and seemed to be induced by elements of the Goeckerman regimen. The hypothesis is presented that these three cases represent a subclinical form of bullous pemphigoid induced by the irritant effects of coal tar, ultraviolet light, the reduced barrier function of a pre-existing dermatosis, or a combination of these factors.

Topics: Aged; Biopsy; Coal Tar; Complement C3; Fibrin; Fluorescent Antibody Technique; Humans; Hydrochlorothiazide; Immunoglobulin G; Male; Middle Aged; Psoriasis; Skin Diseases, Vesiculobullous; Ultraviolet Rays

Topics: Adolescent; Adult; Complement C3; Fibrin; Fluorescent Antibody Technique; Humans; Immunoglobulins; Middle Aged; Psoriasis; Skin

Topics: Amino Acid Sequence; Blood Proteins; Chemical Precipitation; Cold Temperature; Electrophoresis, Polyacrylamide Gel; Fibrin; Fibrinogen; Heparin; Humans; Psoriasis; Skin Diseases

Topics: Arthritis; Cell Membrane; Cell Physiological Phenomena; Endocytosis; Exudates and Transudates; Fibrin; Humans; Inclusion Bodies; Microscopy, Electron; Multiple Sclerosis; Pseudopodia; Psoriasis; Synovial Fluid; Synovial Membrane

Topics: Adolescent; Adult; Aged; Arthritis; Dermatitis, Exfoliative; Eczema; Female; Fibrin; Fluorescent Antibody Technique; Humans; Male; Middle Aged; Photosensitivity Disorders; Psoriasis; Skin; Time Factors

Topics: Beta-Globulins; Electrophoresis; Erythrocytes; Fibrin; Fibrinogen; gamma-Globulins; Hemagglutination; Humans; Immunochemistry; Immunoelectrophoresis; Immunoglobulin G; Keratosis; Lipoproteins; Psoriasis; Serum Albumin; Skin; Skin Diseases; Solubility; Transferrin