fibrin and Pneumoperitoneum

Cell sheet technology is one of the most successful methodologies in regenerative medicine. Various applications of cell sheets have been introduced in first-in-human studies in several clinical fields. When transplanting a cell sheet into internal organs, a relatively large incision is required for delivery due to difficulty handling the sheet. We developed a laparoscopic delivery procedure for safe and easy transplantation of cell sheets in a porcine model.. Pneumoperitoneum was established by inflation with CO. The fibrin-processed myoblast sheet was able to be managed with conventional laparoscopic forceps without breaking. Despite the drastic change in air pressure by passing through the laparoscopic port, the sheets suffered no apparent damage. The transplantation procedure times did not markedly differ among transplant sites. A histopathological examination revealed thin-layered, desmin-positive cells at each transplant site. With transplantation following ESD, the engrafted myoblast sheets effectively prevented delayed perforation.. Our procedure is simple, and the system involves a carrier made of medically fit silicon, commercially available fibrin glue and conventional laparoscopic forceps. Our procedure is a powerful tool for laparoscopical cell sheet transplantation.

Topics: Animals; Cell Transplantation; Endoscopic Mucosal Resection; Fibrin; Fibrin Tissue Adhesive; Laparoscopy; Pneumoperitoneum; Regenerative Medicine; Swine

Topics: Abdomen; Fibrin; Injections, Intraperitoneal; Insufflation; Peritoneal Cavity; Pneumoperitoneum; Pneumoperitoneum, Artificial

Topics: Abdomen; Fibrin; Humans; Insufflation; Peritoneal Cavity; Pneumoperitoneum