fibrin and Lymphoma

Topics: Absorption; Amylases; Antineoplastic Agents; Breast Neoplasms; Bronchial Neoplasms; Capillary Permeability; Carcinoembryonic Antigen; Catheterization; Drainage; Dyspnea; Fibrin; Filtration; Humans; Hydrogen-Ion Concentration; Lymphoma; Pain; Pancreatic Neoplasms; Pleural Effusion; Pleural Neoplasms; Pregnancy Proteins; Quinacrine; Talc; Tetracyclines; Tissue Adhesives; Ultrasonography

Despite the role of curcumin in controlling inflammation, angiogenesis, and cancer in human cells, its therapeutic use is limited. The reasons are quick metabolic breakdown, low aqueous solubility, and bioavailability. This study describes the advantages of clinical-grade curcumin-incorporated fibrin matrix either in lyophilized off-the-shelf wafer or injectable hydrogel forms, as a biodegradable local delivery system. To produce the curcumin-fibrin wafer, used clinical-grade fibrin sealant in a modified composition. To fabricate wafer, we premixed the curcumin with either fibrinogen or thrombin, before clotting into a hydrogel. Sustained release of active curcumin from fibrin wafer, suspended in culture medium at 37 °C lasted for seven days. Upon premixing albumin with thrombin and subsequently adding curcumin into the mixture improved the loading concentration and stability. Dose- and time-dependent apoptotic function of curcumin on cancer cell lines upon release from fibrin wafer, were demonstrated in vitro. In vivo immuno-modulation and a nontoxic response to curcumin released from fibrin into the peritoneal cavity of mice were established. The cytotoxic effect of released curcumin was demonstrated; showing both a preventive and therapeutic role against tumor growth. In vivo studies used Dalton's Lymphoma Ascites (DLA) mice model. Both implanted fibrin wafer and injected hydrogel can breakdown by a physiological process and get cleared by the fibrinolytic mechanism. The lyophilized fibrin wafer could function as a hemostat, adhere to surgical cancer tissues, and arrest bleeding. The potential of curcumin in preventing solid tumor metastasis may be explored upon the sustained delivery of the molecule from the fibrin wafer.

Topics: A549 Cells; Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Ascites; Cell Proliferation; Curcumin; Delayed-Action Preparations; Drug Carriers; Drug Compounding; Drug Liberation; Female; Fibrin; Humans; Hydrogels; Lymphoma; Mice; PC-3 Cells

Hemostatic parameters were examined before and during 102 courses of chemotherapy in 42 patients with malignant lymphoma with high risk for infection. The white blood cell count was significantly reduced in all patients at days 1 and 3, but significantly increased at days 7 and 9, compared to before chemotherapy. At day 7 of chemotherapy, tissue factor (TF) mRNA levels in leukocytes were significantly increased in all patients, especially those with infection. Plasma concentrations of granulocyte elastase derived-XDP (GE-XDP) levels correlated with D-dimer levels during chemotherapy in patients with malignant lymphoma, suggesting that the elevated D-dimer is fibrin products degraded by granulocyte elastase. GE-XDP, C-reactive protein (CRP), GE-XDP and D-dimer were significantly higher in patients with infection, disseminated intravascular coagulation (DIC) and acute respiratory distress syndrome (ARDS) than those without. In patients with DIC or ARDS, TF mRNA correlated with D-dimer, and GE-XDP correlated with leukocyte count, CRP and D-dimer, suggesting that inflammatory changes due to thrombosis may cause the activation of leukocytes in patients with malignant lymphoma during chemotherapy. Activated leukocytes and granulocyte elastase may elicit a hypercoagulable state and ARDS in patients with malignant lymphoma during chemotherapy.

Topics: Aged; Antineoplastic Agents; C-Reactive Protein; Disseminated Intravascular Coagulation; Female; Fibrin; Fibrin Fibrinogen Degradation Products; Fibrinogen; Hemostasis; Humans; Infections; Leukocyte Count; Leukocyte Elastase; Leukocytes; Lymphoma; Male; Middle Aged; Prospective Studies; Respiratory Distress Syndrome; RNA, Messenger; Thromboplastin; Time Factors

Evidence of activation of coagulation was sought in serial plasma samples from 25 ABMT candidates with malignant lymphoma admitted for bone marrow harvesting: 10 females and 15 males, median age 41 years (range 27-58 years). Nineteen patients had non-Hodgkin's lymphoma (NHL) and six had Hodgkin's disease. Of those with NHL, 14 had high-grade and five low- grade disease. The plasma levels of markers of activation (prothrombin fragment 1 + 2, thrombin-antithrombin complexes, fibrinopeptide A and fibrinmonomers) increased significantly (P < 0.001) in association with harvesting. Except for fibrinopeptide A, the indicators of activation were still significantly elevated 24 h after marrow aspiration. Beta-thromboglobulin, a marker of the platelet release reaction, also increased significantly (P < 0.01). Four out of nine patients in whom a long-term central venous catheter was inserted just after marrow aspiration, developed catheter-related deep vein thrombosis, verified venographically, shortly after harvesting. These results suggest that patient with malignant lymphoma undergoing marrow harvesting develop a hypercoagulable state, and that insertion of a central intravenous catheter immediately after marrow harvesting should be avoided to prevent the development of symptomatic deep vein thrombosis.

Topics: Adult; Anticoagulants; Antithrombin III; beta-Thromboglobulin; Biomarkers; Blood Coagulation; Bone Marrow Transplantation; Catheterization, Central Venous; Circadian Rhythm; Female; Fibrin; Fibrinolysis; Fibrinopeptide A; Heparin; Hodgkin Disease; Humans; Ilium; Lymphoma; Lymphoma, Non-Hodgkin; Male; Middle Aged; Peptide Fragments; Peptide Hydrolases; Plasminogen Activator Inhibitor 1; Platelet Count; Premedication; Prothrombin; Sternum; Subclavian Vein; Thrombophlebitis; Transplantation, Autologous; Wounds and Injuries

The purpose of this study was to examine how the intensity of the immunogold labeling on epoxy sections was affected by the use of propylene oxide as an agent in addition to ethanol in the dehydration and infiltration, and also to examine the effect on the immunogold labeling by adding small amounts of propylene oxide to the embedding mixture. Increased knowledge of the mechanism for antigen detection on resin sections was another aim. Thyroid tissue, kidney tissue, and fibrin were embedded in epoxy resin; some with ethanol as the only dehydration agent and others with propylene oxide as an additional agent in dehydration, infiltration or embedding steps in different ways. Immunogold labeling was performed with anti-thyroglobulin, anti-IgG, and anti-fibrinogen, respectively. A higher degree of immunogold labeling was achieved by increasing the concentration of accelerator during infiltration and embedding (Brorson and Skjørten, 1996a, Micron, 27, 211-217). The immunogold labeling of the sections that were based on additional dehydration and infiltration with propylene oxide showed significantly more intense labeling than the sections of tissues that had only been exposed to ethanol in the dehydration and infiltration steps. The embedding of tissues in a mixture of epoxy resin and 5-10% propylene oxide gave higher yields of immunogold labeling than if pure epoxy resin was used for the embedding. The improved labeling is explained by higher amplitudes of protruding antigens on the surface of the sections because antigens are less tightly incorporated in the polymer network when using propylene oxide as additional agent in dehydration, infiltration or embedding. These results illustrate the advantage of using propylene oxide as an additional agent when preparing specimens for immunoelectron microscopy with epoxy resin embedding.

Topics: Animals; Epoxy Compounds; Fibrin; Humans; Immunohistochemistry; Kidney; Lymphoma; Microscopy, Immunoelectron; Swine; Thyroid Gland; Tissue Embedding; Tissue Fixation

A new quantitative method for the study of tumor cell invasion in vitro is presented. It is intended to facilitate the study of the mechanisms of invasion using an isolated basement membrane without the involvement of stromal structures or using defined protein gels. Cells are allowed to migrate through the pores of a Nuclepore polycarbonate filter into a protein gel on a nitrocellulose filter, or they may have to penetrate a cell-derived extracellular matrix (ECM) to reach the gel. Experiments with a nonmetastatic mouse lymphoma (Eb) and its two metastatic variants (ESb and ESb-MP) showed that the metastatic lines penetrated a Matrigel (a gel containing the components of a basement membrane) much better than the nonmetastatic cell line, but only the most metastatic line (ESb) was able to penetrate into a native collagen I gel. The presence of an ECM on the polycarbonate filter reduced the number of cells invading a fibrin gel, demonstrating that the dense, fibrillar structure of the cell-derived ECM was a barrier to the tumor cells. The metastatic lines penetrated the ECM to a 4- to 6-fold higher extent than the nonmetastatic cell line. It is concluded that in order to metastasize efficiently, the tumor cells must be able to penetrate many different kinds of barriers.

Topics: Animals; Cell Adhesion; Collagen; Extracellular Matrix; Fibrin; Gels; Lymphoma; Mice; Neoplasm Invasiveness

Fibrin deposits were observed in the involved lymph nodes and/or spleens of 15 patients with Hodgkin's disease by specific immunofluorescence and by electron microscopy. Two basic patterns of fibrin deposition were observed: 1) intercellular deposits, chiefly associated with nonneoplastic-appearing lymphoid cells and 2) deposits associated with the collagen fibers of young connective tissue. In addition, coarse fibrin deposits were observed in areas of necrosis, presumably a non-specific finding. Fibronectin was also observed in intercellular areas, but staining was less intense than for fibrin. Fibrin deposits were also observed in 3 of 6 cases of non-Hodgkin's lymphoma, indicating that the finding is not an exclusive feature of Hodgkin's disease. The pathogenesis and possible significance of fibrin deposition in Hodgkin's disease are related to earlier observations of activation of the coagulation system on neoplasia and cell-mediated immunity and to the possible role of fibrin, fibronectin, and their breakdown products in angiogenesis and fibroplasia.

Topics: Collagen; Connective Tissue; Fibrin; Fibronectins; Fluorescent Antibody Technique; Hodgkin Disease; Humans; Lymph Nodes; Lymphoma; Microscopy, Electron; Spleen

Topics: Carcinoma, Bronchogenic; Diagnosis, Differential; Fibrin; Humans; Lung Neoplasms; Lymphoma; Mesothelioma; Neoplasm Metastasis; Pleural Diseases; Pleural Neoplasms; Radiography

Topics: Adolescent; Female; Fibrin; Graft Rejection; Humans; Kidney Neoplasms; Kidney Transplantation; Lymphoma; Postoperative Complications; Transplantation, Homologous

Topics: Adolescent; Age Factors; Blood Coagulation Tests; Blood Platelet Disorders; Disseminated Intravascular Coagulation; Factor V; Factor VIII; Fibrin; Fibrinogen; Humans; Kidney Neoplasms; Lymphatic Diseases; Lymphoma; Neoplasm Metastasis; Neoplasms; Neuroblastoma; Osteosarcoma; Retinoblastoma; Rhabdomyosarcoma; Sarcoma, Ewing; Wilms Tumor

Topics: Aged; Allergens; Antibodies; Antibody-Producing Cells; Basement Membrane; Biopsy; Carbon Radioisotopes; Complement System Proteins; Erythema; Fibrin; Fluorescent Antibody Technique; Humans; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Lymphocytes; Lymphoma; Male; Middle Aged; Photosensitivity Disorders; Skin; Skin Tests; Ultraviolet Rays

An immunohistochemical study was performed on the kidneys of 10 subjects with retroperitoneal lymphomas. Kidneys were first examined by light microscopy and six kidneys were further studied by immunofluorescence. Light microscopic evidence of damage found in 6 of 10 subjects was characterized by thickening of the basement membrane of glomerular capillaries and an increase in the mesangial matrix. In the areas of light microscopically visible damage, deposits of Schiff-positive material and argyrophilic material were present. No significant changes were found in the tubules. Four of the six kidneys which showed damage by light microscopy showed further evidence of histologic change in areas of glomerular damage when examined using the direct immunofluorescence technic. In 2 subjects, diffuse deposits of IgG and beta1C-beta1A globulin in basement membranes and the mesangial region were prominent, while in the remaining 2 subjects IgG and beta1C-beta1A globulin were localized as linear and/or beaded deposits, both inside and outside the basement membranes. Only focal deposits of these conjugates were seen in the mesangium. No localization of IgA, IgM, rabbit antihuman fibrin or fibrinogen was noted in any of the cases. The tubules yielded negative reactions for the localization of any of the conjugates. The results of this study seem to support the concept that, in some cases of retroperitoneal lymphomas, clinical and renal functional disturbances may be explained on the basis of an immunologic injury to the basement membranes of the glomeruli.

Topics: Basement Membrane; Complement System Proteins; Fibrin; Fibrinogen; Fluorescent Antibody Technique; Humans; Immunoglobulin G; Immunoglobulin M; Kidney Glomerulus; Lymphoma; Retroperitoneal Neoplasms

Topics: Adhesiveness; Animals; Blood Platelets; Blood Vessels; Carcinoma 256, Walker; Embolism; Epithelium; Fibrin; Lymphoma; Microscopy, Electron; Neoplasm Metastasis; Neoplasm Transplantation; Neoplasms, Experimental; Neoplastic Cells, Circulating; Rats; Surface Properties

Topics: Alpha-Globulins; Anemia; Biological Assay; Biomedical Research; Blood Coagulation; Blood Coagulation Factors; Calcium; Collagen Diseases; Cysteine; Factor XIII; Fibrin; Hemorrhagic Disorders; Humans; Leukemia; Liver; Liver Diseases; Lymphoma; Neoplasms; Pathology; Serum Globulins; Sulfhydryl Compounds; Thrombin

Topics: Animals; Antibodies; Fibrin; Immunoglobulins; Lymphoma; Lymphoma, Non-Hodgkin; Neoplasms; Rats

Topics: Fibrin; Fibrinolysis; Leukemia; Lymphoma; Neoplasms