fibrin and Hemangioma

Fine needle aspiration biopsy (FNAB) can cause reactive histopathological changes, commonly including haemorrhage and granulation tissue. The literature describing vascular proliferation after FNAB is sparse. We aimed to describe neovascularisation in thyroid gland specimens as a consequence of FNAB.. We analysed all thyroid histopathological specimens from the Fimlab Laboratories collected between 2010 and 2013 for neovascularisation and distortions in the accompanying tissue. We evaluated HE-stained slides and CD31-, podoplanin-, and Ki-67-immunostained slides.. We observed vascular proliferation in 64 out of 787 specimens (8.1%). In these patients, the mean age was 62 years, 43 were female and 21 were male. Previous FNAB data were available in 49 cases (76.6%). In 51 cases (79.7%), the neovascularisation occupied less than 5% of the thyroid gland area. The vessel dilatation was moderate in 28 cases (43.8%) and low in 20 cases (31.3%). In tumours, neovessels were detected within the tumour and in the surrounding tissue.. Post-FNAB tissue samples include dilated newly formed vessels, which pathologists should differentiate from rare thyroid vascular tumours. The proposed mechanism is a traumatically induced haemorrhage followed by haematoma and thrombosis that resolves by recanalisation. A knowledge of tissue alteration is needed to avoid misdiagnoses.

Topics: Adult; Aged; Aged, 80 and over; Biopsy, Fine-Needle; Cell Proliferation; Edema; Female; Fibrin; Hemangioma; Hemorrhage; Humans; Immunohistochemistry; Male; Middle Aged; Neovascularization, Pathologic; Thyroid Gland

Haemangiomas are benign tumours occurring in up to 12% of Caucasians, particularly in infancy and childhood. In the present study, two variant cell lines were isolated from murine endothelioma cells. One variant, named t.End.1V(high), represented 16.9% of the parental cell population and was selected by virtue of high expression levels of integrin alphavbeta3 and reduced capacity to endocytose acetylated low-density lipoproteins (Ac-LDLs). A second variant, named t.End.1V(low), represented 38.8% of the parental endothelioma cell line, expressed low levels of alphavbeta3 integrin, and was able to endocytose Ac-LDL. These phenotypic modifications were stable and correlated with specific morphological and functional properties of the two variant cell lines. While the t.End.1V(high) cells induced the formation of large haemangiomas when injected subcutaneously into mice, the t.End.1V(low) cells formed haemangiocytomas. When compared with t.End.1V(low) cells, the t.End.1V(high) cells showed increased migratory capacity, lacked an inflammatory response, and formed cord-like structures in fibrin gels. In contrast, the t.End.1V(low) cells organized into cysts with a lumen in fibrin gels. They rarely formed blood-filled haemangiomas in vivo and recruited host smooth muscle cells, a phenomenon typical for vessel wall maturation of resting cells. These data suggest that Ac-LDL uptake and the level of alphavbeta3 integrin expression are linked to the ability of endothelial cells to form large haemangiomas in vivo.

Topics: Animals; Cell Line, Tumor; Cell Movement; Culture Media; Endothelial Cells; Endothelium, Vascular; Enzyme Inhibitors; Fibrin; Hemangioma; Integrin alphaVbeta3; Lipoproteins, LDL; Mice; Muscle, Smooth, Vascular

Fibrin is found in most solid tumours, and there is speculation regarding its role in tumour invasion and metastasis. An assay to quantitate fibrin levels in tissues would be a useful preliminary tool in assessing the above. Such an assay to quantitatively detect levels of fibrin in various types of canine tumour was developed. This procedure involved an ELISA using a monoclonal antibody (MAb 1H10) for canine fibrin as a capture antibody and a polyclonal antibody to human fibrinogen conjugated to horseradish peroxidase as the detection antibody. The ELISA is calibrated against known concentrations of freeze-fractured fibrin derived from clotted dog plasma. The assay takes 3.5 hours, and concentrations as low as 0.1 microg fibrin per milliliter of solubilised tumour can be readily detected. ELISA dilution curves for fibrin from various types of canine tumour were found to be parallel to the standard canine fibrin calibration curve. The intraassay and interassay variabilities of the assay gave coefficients of variation in the range of 2.4-4.5% and 7.2-7.8%, respectively, for the calibrator standard, in a concentration range of 0.1-10 microg/ml. Using this assay, we reported the levels of fibrin in three different types of malignant canine tissue.

Topics: Animals; Antibody Specificity; Dog Diseases; Dogs; Enzyme-Linked Immunosorbent Assay; Female; Fibrin; Freeze Fracturing; Hemangioma; Kidney Neoplasms; Male; Mammary Neoplasms, Animal; Neoplasms

The arachnoideal compartment provides the vascular sources for three different tumor types rich in vessels: angiomatous meningioma, some atypical meningioma with high vascularity and meningeal hemangiopericytoma. We investigated immunohistochemically the expression and distribution of vascular mitogenes in 7 angiomatous meningiomas, 8 atypical meningiomas with high vasculature and 4 hemangiopericytomas. On the one hand it should be studied which vascular growth factors such as VPF/VEGF-1, VPF/VEGF-2, bFGF, PDGF and TGF-alpha could be responsible for the close meshwork of vessels within the tumors. On the other hand we were interested in whether or not there are differences in vascular mitogens between slowly growing angiomatous meningiomas and both other types with their increased tendency to recur. PDGF and TGF-alpha were extensively expressed in the endothelium and smooth muscle cells of the vessels, as well as tumor cells. VEGF-2 could only be found in endothelial cells of all three tumor entities. bFGF was localized in some vessels of angiomatous meningiomas and VEGF-1 revealed a very low expression with a localization comparable with VEGF-2. Moreover, uPAR was diffusely expressed in nearly all tumor cells and endothelial cells. The fact that tumor cells of hemangiopericytomas and meningiomas did not show any immunohistochemical reaction with VEGF's could indicate a lower priority of these growth factors for neovascularization in this type of neoplasm. A different expression of vascular mitogens between benign angiomatous meningiomas and atypical meningiomas as well as hemangiopericytomas with their tendency for recurrence could not be observed. The morphological evidence for extravasates of IgG-proteins, Fibrin and Fibronectin due to VPF-effects seems not to be a renouncable condition for neoangiogenesis in the tumors investigated.

Topics: Adult; Aged; Aged, 80 and over; Biomarkers, Tumor; Endothelial Growth Factors; Endothelium, Vascular; Female; Fibrin; Fibroblast Growth Factor 2; Growth Substances; Hemangioma; Hemangiopericytoma; Humans; Immunohistochemistry; Lymphokines; Male; Meningioma; Middle Aged; Muscle, Smooth, Vascular; Platelet-Derived Growth Factor; Transforming Growth Factor alpha; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors

Expression of the polyoma virus middle T (mT) oncogene in vivo is associated with a profound subversion of normal vascular development, which results in the formation of endothelial tumors (hemangiomas). In an attempt to understand the molecular mechanisms responsible for this phenomenon, we have investigated, in an in vitro system, the morphogenetic properties of endothelial cells expressing this oncogene. mT-expressing endothelioma (End) cells grown within fibrin gels formed large hemangioma-like cystic structures. All End cell lines examined expressed high levels of fibrinolytic activity resulting from increased production of urokinase-type plasminogen activator and decreased production of plasminogen activator inhibitors. Neutralization of excess proteolytic activity by exogenously added serine protease inhibitors corrected the aberrant in vitro behavior of End cells and allowed the formation of capillary-like tubules. These results suggest that tightly controlled proteolytic activity is essential for vascular morphogenesis and that physiological protease inhibitors play an important regulatory role in angiogenesis.

Topics: Animals; Antigens, Polyomavirus Transforming; Cell Line; Cells, Cultured; Endothelium, Vascular; Fibrin; Fibrinolysis; Gene Expression; Hemangioma; Humans; Mice; Morphogenesis; Oncogenes; Plasminogen Activators; Plasminogen Inactivators; RNA, Messenger; Tumor Cells, Cultured

Topics: Abruptio Placentae; Calcinosis; Female; Fibrin; Hemangioma; Humans; Hydatidiform Mole; Infarction; Placenta; Placenta Diseases; Pregnancy; Thrombosis; Ultrasonography; Uterine Neoplasms

The clinicopathologic features of 50 noninfiltrating angiolipomas are presented, with a detailed ultrastructural study of five cases and immunoflourescence study of one case. The clinical presentation, including age, location, and associated symptoms, is discussed. Pertinent light microscopic findings, including features helpful in diagnosing borderline lesions, are described. The first ultrastructural and immunofluorescence study of this entity is presented. Among various ultrastructural findings, there appear to be decreased numbers of Weibel-Palade bodies in the endothelial cells of all angiolipomas studied. The relationship of Weibel-Palade bodies to the functional state of the cell and their possible procoagulative effect are discussed. Immunofluorescence and ultrastructural studies further suggest that endothelial disruption and endothelial fibrinogen contribute to the fibrin thrombus formation typically found in angiolipomas.

Topics: Adipose Tissue; Adult; Aged; Blood Coagulation; Blood Vessels; Capillaries; Cytoplasm; Endothelium; Fibrin; Fluorescent Antibody Technique; Hemangioma; Humans; Lipoma; Male; Middle Aged

Topics: Adult; Aged; Aneurysm; Aortic Aneurysm; Arteriosclerosis; Blood Cell Count; Blood Coagulation; Blood Platelets; Buttocks; Cell Survival; Chromium Radioisotopes; Chronic Disease; Disseminated Intravascular Coagulation; Female; Fibrin; Fibrinogen; Hemangioma; Humans; Iodine Radioisotopes; Middle Aged; Purpura, Thrombocytopenic; Skin Neoplasms; Syndrome; Thrombosis

Topics: Adenoma; Carcinoma; Culture Media; Culture Techniques; Cyclohexanecarboxylic Acids; Fibrin; Fibrinogen; Fibrinolysis; Fibroma; Granuloma; Granuloma, Giant Cell; Hemangioma; Humans; Methylamines; Mouth Neoplasms; Neoplasms, Muscle Tissue

Topics: Anemia; Animals; Blood Platelets; Disease Models, Animal; Erythrocytes, Abnormal; Fibrin; Hemangioma; Mice; Microscopy, Electron, Scanning; Neoplasm Transplantation; Neoplasms, Experimental; Purpura, Thrombocytopenic; Skin; Skin Neoplasms; Transplantation, Homologous