fibrin and Carcinoma--Ductal--Breast

Lysyl oxidase (LO) initiates the first step in the crosslinking of collagens and elastin and has also been shown to function as a tumor suppressor. The purpose of the present work was to determine whether the products of a newly described LO-like gene (LOXL) that encodes a close homolog of LO, the LO-like (LOL) protein, is associated with extracellular matrix remodeling during fibrotic disorders. Specific antibody against LOL identified proteins of approximately 30, 42, 52 and 68 kd in various cells and in bovine aorta. These proteins were immunochemically distinct from the recombinant LO expressed by fibroblasts and from the bovine aorta LO. The LO gene (LOX) and LOXL were transiently up-regulated at early stages of liver granuloma development in Schistosoma mansoni-infected mice, although the peak of LOL mRNA synthesis preceded that of LO. LOL protein and LO were colocalized at sites of fibrogenesis in human lung fibrosis and in the stromal reaction of bronchiolo-alveolar carcinomas and of in situ ductal breast tumors. In conclusion, the LOL protein was identified as a secreted protein and localized in the extracellular matrix in active fibrotic diseases and in the early stromal reaction of breast cancer.

Topics: Adenocarcinoma, Bronchiolo-Alveolar; Animals; Carcinoma, Ductal, Breast; Cattle; Cell Line; Female; Fibrin; HeLa Cells; Humans; Liver Diseases, Parasitic; Lung; Lung Neoplasms; Mammary Neoplasms, Animal; Mice; Peptide Fragments; Protein-Lysine 6-Oxidase; Pulmonary Fibrosis; RNA, Messenger; Schistosomiasis mansoni; Stromal Cells

The aim of our study was to explain the effect of elevated homocysteine (measured by HPLC) on haemostatic activity of plasma from breast cancer patients (fibrin polymerization and lysis; the thrombin and prothrombin time), because homocysteine (Hcys) induces changes in haemostasis, as well blood clotting as fibrinolysis. Patients were hospitalized in Department of Oncological Surgery, Medical University of Lodz, Poland. All patients have not had preadjuvant therapy, and samples from patients were taken before surgery. We observed that changes of selected parameters of haemostatic properties of plasma, e.g., the prothrombin time and thrombin time were prolonged in plasma from invasive breast cancer when compared with the control group (healthy subjects) and patients with benign breast diseases. Our results showed also that the correlation between the increased amount of Hcys and changes of selected parameters of haemostasis in invasive breast cancer patients exists. Considering the data presented in this study, we suggest that the elevated Hcys in invasive breast cancer patients may induce the changes of haemostatic properties of plasma isolated from these patients.

Topics: Adult; Aged; Aged, 80 and over; Breast Neoplasms; Carcinoma, Ductal, Breast; Case-Control Studies; Female; Fibrin; Fibrinolysis; Hemostasis; Homocysteine; Humans; Middle Aged; Risk Factors; Statistics, Nonparametric; Thrombin Time

Seven patients with mammographic lesions entirely removed at percutaneous core needle biopsy that required wider excision underwent freehand localization of the site of the prior lesion with orthogonal and reproducible mammographic landmarks to guide needle placement. Successful excision was accomplished in all cases, as evidenced by similar histopathologic findings, fibrin bands or collagen, and core needle biopsy tract at microscopy.

Topics: Aged; Biopsy, Needle; Breast; Breast Neoplasms; Carcinoma in Situ; Carcinoma, Ductal, Breast; Collagen; Coloring Agents; Feasibility Studies; Female; Fibrin; Follow-Up Studies; Humans; Hyperplasia; Mammography; Methylene Blue; Middle Aged; Needles; Neoplasm, Residual; Preoperative Care; Radiography, Interventional; Stereotaxic Techniques