fg-4592 and Femoral-Neck-Fractures

Fracture site is regionally hypoxic resulting from vasculature disruption. HIF-1αplays an essential role in fracture repair. This study aims to investigate the influence of FG4592 on the femur fracture of SD rats and the proliferation, migration of BMSCs.. After the femoral fracture model was established, computed tomography imaging and histological analyses were used to quantify bone healing and the expression of CD90, HIF-1α, VEGF were observed by means of immunohistochemistry method on Day 10 and Day 20. In addition, CCK-8 assay, transwell, flow cytometric analysis, laser confocal microscopy assay, western blot and rT-PCR were performed to text the proliferation and migration of BMSCs using FG4592.. In vivo, FG4592 facilitated the repair of bone fracture by increasing the number of BMSCs and cartilage formation. In vitro, FG4592 markedly improved the proliferation, migration of BMSCs via upregulation of intracellular Ca

Topics: Animals; Bone Marrow Cells; Cell Movement; Cell Proliferation; Cells, Cultured; Femoral Neck Fractures; Fracture Healing; Glycine; Hypoxia-Inducible Factor 1, alpha Subunit; Isoquinolines; Male; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Nitric Oxide; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species