ferrous-citrate and Iron-Overload

ferrous-citrate has been researched along with Iron-Overload* in 2 studies

Other Studies

2 other study(ies) available for ferrous-citrate and Iron-Overload

Article	Year
Relationship between the Induced Iron Overload Model and Hepatic Erythropoiesis in Zoological science, 2020, Volume: 37, Issue:1 Topics: Animals; Citric Acid; Erythropoiesis; Ferrous Compounds; Iron; Iron Overload; Liver; Male; Models, Animal; Xenopus laevis	2020
Iron excess limits HHIPL-2 gene expression and decreases osteoblastic activity in human MG-63 cells. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2012, Volume: 23, Issue:10 In order to understand mechanisms involved in osteoporosis observed during iron overload diseases, we analyzed the impact of iron on a human osteoblast-like cell line. Iron exposure decreases osteoblast phenotype. HHIPL-2 is an iron-modulated gene which could contribute to these alterations. Our results suggest osteoblast impairment in iron-related osteoporosis.. Iron overload may cause osteoporosis. An iron-related decrease in osteoblast activity has been suggested.. We investigated the effect of iron exposure on human osteoblast cells (MG-63) by analyzing the impact of ferric ammonium citrate (FAC) and iron citrate (FeCi) on the expression of genes involved in iron metabolism or associated with osteoblast phenotype. A transcriptomic analysis was performed to identify iron-modulated genes.. FAC and FeCi exposure modulated cellular iron status with a decrease in TFRC mRNA level and an increase in intracellular ferritin level. FAC increased ROS level and caspase 3 activity. Ferroportin, HFE and TFR2 mRNAs were expressed in MG-63 cells under basal conditions. The level of ferroportin mRNA was increased by iron, whereas HFE mRNA level was decreased. The level of mRNA alpha 1 collagen type I chain, osteocalcin and the transcriptional factor RUNX2 were decreased by iron. Transcriptomic analysis revealed that the mRNA level of HedgeHog Interacting Protein Like-2 (HHIPL-2) gene, encoding an inhibitor of the hedgehog signaling pathway, was decreased in the presence of FAC. Specific inhibition of HHIPL-2 expression decreased osteoblast marker mRNA levels. Purmorphamine, hedgehog pathway activator, increased the mRNA level of GLI1, a target gene for the hedgehog pathway, and decreased osteoblast marker levels. GLI1 mRNA level was increased under iron exposure.. We showed that in human MG-63 cells, iron exposure impacts iron metabolism and osteoblast gene expression. HHIPL-2 gene expression modulation may contribute to these alterations. Our results support a role of osteoblast impairment in iron-related osteoporosis. Topics: Cation Transport Proteins; Cells, Cultured; Citric Acid; Ferric Compounds; Ferrous Compounds; Gene Expression Regulation; Hemochromatosis Protein; Histocompatibility Antigens Class I; Humans; Iron Overload; Membrane Proteins; Osteoblasts; Oxidative Stress; Phenotype; Quaternary Ammonium Compounds	2012

Article

Year

Relationship between the Induced Iron Overload Model and Hepatic Erythropoiesis in

Zoological science, 2020, Volume: 37, Issue:1

Topics: Animals; Citric Acid; Erythropoiesis; Ferrous Compounds; Iron; Iron Overload; Liver; Male; Models, Animal; Xenopus laevis

2020

Iron excess limits HHIPL-2 gene expression and decreases osteoblastic activity in human MG-63 cells.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 2012, Volume: 23, Issue:10

In order to understand mechanisms involved in osteoporosis observed during iron overload diseases, we analyzed the impact of iron on a human osteoblast-like cell line. Iron exposure decreases osteoblast phenotype. HHIPL-2 is an iron-modulated gene which could contribute to these alterations. Our results suggest osteoblast impairment in iron-related osteoporosis.. Iron overload may cause osteoporosis. An iron-related decrease in osteoblast activity has been suggested.. We investigated the effect of iron exposure on human osteoblast cells (MG-63) by analyzing the impact of ferric ammonium citrate (FAC) and iron citrate (FeCi) on the expression of genes involved in iron metabolism or associated with osteoblast phenotype. A transcriptomic analysis was performed to identify iron-modulated genes.. FAC and FeCi exposure modulated cellular iron status with a decrease in TFRC mRNA level and an increase in intracellular ferritin level. FAC increased ROS level and caspase 3 activity. Ferroportin, HFE and TFR2 mRNAs were expressed in MG-63 cells under basal conditions. The level of ferroportin mRNA was increased by iron, whereas HFE mRNA level was decreased. The level of mRNA alpha 1 collagen type I chain, osteocalcin and the transcriptional factor RUNX2 were decreased by iron. Transcriptomic analysis revealed that the mRNA level of HedgeHog Interacting Protein Like-2 (HHIPL-2) gene, encoding an inhibitor of the hedgehog signaling pathway, was decreased in the presence of FAC. Specific inhibition of HHIPL-2 expression decreased osteoblast marker mRNA levels. Purmorphamine, hedgehog pathway activator, increased the mRNA level of GLI1, a target gene for the hedgehog pathway, and decreased osteoblast marker levels. GLI1 mRNA level was increased under iron exposure.. We showed that in human MG-63 cells, iron exposure impacts iron metabolism and osteoblast gene expression. HHIPL-2 gene expression modulation may contribute to these alterations. Our results support a role of osteoblast impairment in iron-related osteoporosis.

Topics: Cation Transport Proteins; Cells, Cultured; Citric Acid; Ferric Compounds; Ferrous Compounds; Gene Expression Regulation; Hemochromatosis Protein; Histocompatibility Antigens Class I; Humans; Iron Overload; Membrane Proteins; Osteoblasts; Oxidative Stress; Phenotype; Quaternary Ammonium Compounds

2012