ferric-ammonium-citrate and Chemical-and-Drug-Induced-Liver-Injury

Liver slices of turpentine-treated rats were incubated in vitro and used as a model to study synthesis and secretion of proteins during the acute-phase response. The synthesis and secretion of typical acute-phase proteins increased after treatment. Similarly, ferritin increased at 24-48 hr after treatment. Serum ferritin showed a slight and transient increase at 6 hr; however, no ferritin was detectable in liver slices medium, indicating no or negligible secretion by this tissue. Northern blot analysis of RNA extracted from total liver homogenate and from free and membrane-bound polyribosomes revealed that turpentine treatment stimulates ferritin synthesis at the translational level, possibly increasing the amount of ferritin mRNA on membrane-bound polysomes.

Topics: Acute-Phase Proteins; Animals; Chemical and Drug Induced Liver Injury; Ferric Compounds; Ferritins; Gene Expression Regulation; Inflammation; Iron; Liver; Male; Quaternary Ammonium Compounds; Rats; Rats, Inbred Strains; RNA, Messenger; Turpentine