exendin-3 and Hyperglycemia

Targeting the glucagon-like peptide-1 receptor with radiolabeled exendin is a very promising method to noninvasively determine the β cell mass in the pancreas, which is needed to unravel the pathophysiology of type 1 and type 2 diabetes. The present study aimed to explore the effects of both hyperglycemia and insulitis on the uptake of exendin in a spontaneous type 1 diabetes mouse model, nonobese diabetic (NOD) mice.. NOD mice (. The pancreas could be visualized longitudinally using SPECT. A linear correlation was found between the BCM (%) and pancreatic uptake (%ID/g) as measured by ex vivo counting (Pearson. Despite hyperglycemia and severe insulitis, we have found a good correlation between BCM and pancreatic exendin uptake, even in a suboptimal model with relatively high background activity.

Topics: Animals; Autoradiography; Diabetes Mellitus, Type 1; Disease Models, Animal; Female; Hyperglycemia; Immunohistochemistry; Indium Radioisotopes; Injections, Intravenous; Insulin-Secreting Cells; Mice; Mice, Inbred NOD; Pentetic Acid; Peptides; Radiopharmaceuticals; Tissue Distribution; Tomography, Emission-Computed, Single-Photon