eunicellin and Neoplasms

Three new polyoxygenated diterpenoids with a rare 4-isopropyl-1,5,8a-trimethylperhydrophenanthrane structure of the klysimplexane skeleton, briarols A‒C (1‒3), and one eunicellin-based diterpenoid, briarol D (4), were isolated from

Topics: Animals; Anthozoa; Antineoplastic Agents; Cell Line, Tumor; Chemistry, Pharmaceutical; Diterpenes; Drug Design; HT29 Cells; Humans; Inhibitory Concentration 50; Magnetic Resonance Spectroscopy; Neoplasms; Powders; Protons; Spectrometry, Mass, Electrospray Ionization; Taiwan; Water Pollutants, Chemical; Water Purification

Five new eunicellin-based diterpenoids, krempfielins E-I (1-5) and seven known compounds (6-12) were isolated from the organic extract of a Taiwanese soft coral Cladiella krempfi. The structures of the new metabolites were elucidated on the basis of extensive spectroscopic analysis. Metabolites 5, 6, 10 and 12 were shown to exhibit cytotoxicity against a limited panel of cancer cell lines. Furthermore, compounds 6 and 10 could potently inhibit the accumulation of the pro-inflammatory iNOS protein, and 6 and 12 could significantly reduce the expression of COX-2 protein in LPS-stimulated RAW264.7 macrophage cells.

Topics: Animals; Anthozoa; Anti-Inflammatory Agents; Antineoplastic Agents; Cell Line, Tumor; Cyclooxygenase 2; Diterpenes; Humans; Inflammation; Lipopolysaccharides; Macrophages; Mice; Neoplasms; Nitric Oxide Synthase Type II; Spectrum Analysis; Taiwan