eudesmin and Lung-Neoplasms

Limonoids possess broad range of biological activities, including antitumour, antimicrobial and antioxidant activities, etc. Eudesmin (EDN) is a type of limonoid which also possesses various activities. However, there is no report on the antitumour lung cancer (LC) activities of this compound.. The present study investigates the antitumour effects of EDN and its potential molecular mechanisms.. The in vitro antitumour effects of EDN on LC A549 cells were evaluated by using MTT assay. The in vivo antitumour effects were investigated on a xenograft athymic nude mouse model. The mice were administered orally with EDN (10, 20 and 40 mg/kg) once daily for 28 days. Effects of EDN on apoptosis-related or signalling proteins (Bcl-2, Bax, caspase-3, caspase-9, P53, Akt and JNK) were assayed by western blot analysis.. Overall, the results indicated that EDN possesses significant antitumour effects on LC and the possible mechanism might be related to induction of mitochondria-mediated apoptosis.

Topics: A549 Cells; Administration, Oral; Animals; Antineoplastic Agents, Phytogenic; Apoptosis; Blotting, Western; Dose-Response Relationship, Drug; Down-Regulation; Furans; Gene Expression Regulation, Neoplastic; Humans; Inhibitory Concentration 50; Lignans; Lung Neoplasms; Male; Mice; Mice, Nude; Mitochondria; Up-Regulation; Xenograft Model Antitumor Assays