ethidium and Necrosis
ethidium has been researched along with Necrosis in 26 studies
Ethidium: A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.
ethidium : The fluorescent compound widely used in experimental cell biology and biochemistry to reveal double-stranded DNA and RNA.
Necrosis: The death of cells in an organ or tissue due to disease, injury or failure of the blood supply.
Research Excerpts
Excerpt | Relevance | Reference |
---|---|---|
" Genotoxic and cytotoxic effects of ascorbic acid and β-carotene were evaluated by alkali single cell gel electrophoresis (SCGE), acridine orange/ethidium bromide staining patterns of cells (apoptosis and necrosis) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS)." | 7.77 | Effects of ascorbic acid and β-carotene on HepG2 human hepatocellular carcinoma cell line. ( Iseri, OD; Sahin, FI; Yurtcu, E, 2011) |
"Ethidium homodimer is a cell-membrane impermeant nuclear fluorochrome that has been widely used to identify necrotic cells in culture." | 5.34 | A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer. ( Diamantakos, EA; Edwards, JR; Lamar, PC; Peuler, JD; Prozialeck, WC, 2007) |
" Genotoxic and cytotoxic effects of ascorbic acid and β-carotene were evaluated by alkali single cell gel electrophoresis (SCGE), acridine orange/ethidium bromide staining patterns of cells (apoptosis and necrosis) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS)." | 3.77 | Effects of ascorbic acid and β-carotene on HepG2 human hepatocellular carcinoma cell line. ( Iseri, OD; Sahin, FI; Yurtcu, E, 2011) |
" Fluorescence microscopy using AO/EB (acridine orange/ethidium bromide) staining indicated that KA can induce both apoptosis and necrosis in HL-60 cell cultures, which corroborate the findings with MTT." | 3.75 | Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells. ( Bezerra, DP; Câmara, CA; Cavalcanti, BC; Costa-Lotufo, LV; Lima, MA; Magalhães, HI; Moraes, MO; Pessoa, C; Rao, VS; Silveira, ER, 2009) |
"An automated fluorescence method for the detection of neuronal cell death by necrosis and apoptosis with sequential acridine orange (AO) and ethidium bromide (EB) staining using confocal microscopy is described." | 3.74 | A fluorescence vital assay for the recognition and quantification of excitotoxic cell death by necrosis and apoptosis using confocal microscopy on neurons in culture. ( Antonov, SM; Evstratova, AA; Mironova, EV, 2007) |
"The cytotoxic effects of photo-irradiated riboflavin and rose bengal are shown to be mediated by two distinct but parallel pathways, one leading to apoptosis and the other to necrosis." | 3.70 | Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. ( Cho, KS; Choi, JS; Joo, CK; Lee, EH, 1999) |
"The reliability of eight distinct methods (Giemsa staining, trypan blue exclusion, acridine orange/ethidium bromide (AO/EB) double staining for fluorescence microscopy and flow cytometry, propidium iodide (PI) staining, annexin V assay, TUNEL assay and DNA ladder) for detection and quantification of cell death (apoptosis and necrosis) was evaluated and compared." | 3.70 | Critical evaluation of techniques to detect and measure cell death--study in a model of UV radiation of the leukaemic cell line HL60. ( Guimarães, JE; Leite, M; Leite, PS; Quinta-Costa, M, 1999) |
"Ethidium homodimer is a cell-membrane impermeant nuclear fluorochrome that has been widely used to identify necrotic cells in culture." | 1.34 | A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer. ( Diamantakos, EA; Edwards, JR; Lamar, PC; Peuler, JD; Prozialeck, WC, 2007) |
"We hypothesise that hearing impairment associated with DFNA5 is caused by a highly unusual mechanism, in which skipping of one specific exon leads to disease that is not caused by other mutations in this gene." | 1.32 | DFNA5: hearing impairment exon instead of hearing impairment gene? ( Smith, RJ; Thys, S; Timmermans, JP; Van Bockstaele, DR; Van Camp, G; Van Laer, L; Van Tendeloo, VF; Vrijens, K, 2004) |
Research
Studies (26)
Timeframe | Studies, this research(%) | All Research% |
---|---|---|
pre-1990 | 1 (3.85) | 18.7374 |
1990's | 4 (15.38) | 18.2507 |
2000's | 17 (65.38) | 29.6817 |
2010's | 3 (11.54) | 24.3611 |
2020's | 1 (3.85) | 2.80 |
Authors
Authors | Studies |
---|---|
Hosseini, A | 1 |
Shetab-Boushehri, SM | 1 |
Shetab-Boushehri, SV | 1 |
Tomczyk, M | 1 |
Drozdowska, D | 1 |
Bielawska, A | 1 |
Bielawski, K | 1 |
Gudej, J | 1 |
Tapajós, EC | 1 |
Longo, JP | 1 |
Simioni, AR | 1 |
Lacava, ZG | 1 |
Santos, MF | 1 |
Morais, PC | 1 |
Tedesco, AC | 1 |
Azevedo, RB | 1 |
Cavalcanti, BC | 1 |
Bezerra, DP | 1 |
Magalhães, HI | 1 |
Moraes, MO | 1 |
Lima, MA | 1 |
Silveira, ER | 1 |
Câmara, CA | 1 |
Rao, VS | 1 |
Pessoa, C | 1 |
Costa-Lotufo, LV | 1 |
Yurtcu, E | 1 |
Iseri, OD | 1 |
Sahin, FI | 1 |
Baraba, A | 1 |
Zelježić, D | 1 |
Kopjar, N | 1 |
Mladinić, M | 1 |
Anić, I | 1 |
Miletić, I | 1 |
Hao, B | 1 |
Cheng, S | 1 |
Clancy, CJ | 1 |
Nguyen, MH | 1 |
Ho, JD | 1 |
Tsai, RJ | 1 |
Chen, SN | 1 |
Chen, HC | 1 |
Rasilainen, S | 1 |
Ylipaasto, P | 1 |
Roivainen, M | 1 |
Lapatto, R | 1 |
Hovi, T | 1 |
Otonkoski, T | 1 |
Van Laer, L | 1 |
Vrijens, K | 1 |
Thys, S | 1 |
Van Tendeloo, VF | 1 |
Smith, RJ | 1 |
Van Bockstaele, DR | 1 |
Timmermans, JP | 1 |
Van Camp, G | 1 |
Park, LO | 1 |
Yoon, JH | 1 |
Kim, JD | 1 |
Seo, JT | 1 |
Bae, SN | 1 |
Kosmider, B | 1 |
Zyner, E | 1 |
Osiecka, R | 1 |
Ochocki, J | 1 |
Barnett, MJ | 1 |
McGhee-Wilson, D | 1 |
Shapiro, AM | 1 |
Lakey, JR | 1 |
Ribble, D | 1 |
Goldstein, NB | 1 |
Norris, DA | 1 |
Shellman, YG | 1 |
Auger, R | 1 |
Motta, I | 1 |
Benihoud, K | 1 |
Ojcius, DM | 1 |
Kanellopoulos, JM | 1 |
Huang, R | 1 |
Mendis, E | 1 |
Rajapakse, N | 1 |
Kim, SK | 1 |
Oliveira, RJ | 1 |
Matuo, R | 1 |
da Silva, AF | 1 |
Matiazi, HJ | 1 |
Mantovani, MS | 1 |
Ribeiro, LR | 1 |
Edwards, JR | 1 |
Diamantakos, EA | 1 |
Peuler, JD | 1 |
Lamar, PC | 1 |
Prozialeck, WC | 1 |
Mironova, EV | 1 |
Evstratova, AA | 1 |
Antonov, SM | 1 |
Redman, SN | 1 |
Khan, IM | 1 |
Tew, SR | 1 |
Archer, CW | 1 |
Waring, P | 1 |
Sjaarda, A | 1 |
Liegler, TJ | 1 |
Hyun, W | 1 |
Yen, TS | 1 |
Stites, DP | 1 |
Cho, KS | 1 |
Lee, EH | 1 |
Choi, JS | 1 |
Joo, CK | 1 |
Leite, M | 1 |
Quinta-Costa, M | 1 |
Leite, PS | 1 |
Guimarães, JE | 1 |
Estacion, M | 1 |
Schilling, WP | 1 |
Afanas'ev, VN | 1 |
Korol', BA | 1 |
Mantsygin, IuA | 1 |
Nelipovich, PA | 1 |
Pechatnikov, VA | 1 |
Other Studies
26 other studies available for ethidium and Necrosis
Article | Year |
---|---|
Evaluation of Cytotoxic, Necrotic, Apoptotic, and Autophagic Effects of Methamphetamine and 3,4-Methylenedioxymethamphetamine on U-87 MG (Glial) and B104-1-1 (Neuronal) Cell Lines.
Topics: Acridine Orange; Cell Line; Central Nervous System Stimulants; Designer Drugs; Ethidium; Humans; Met | 2022 |
Human DNA topoisomerase inhibitors from Potentilla argentea and their cytotoxic effect against MCF-7.
Topics: Antineoplastic Agents, Phytogenic; Apoptosis; Benzopyrans; Camptothecin; Cell Line, Tumor; DNA; DNA, | 2008 |
In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine.
Topics: Acridine Orange; Cell Survival; Coloring Agents; DNA Fragmentation; Ethidium; Fluorescent Dyes; Huma | 2008 |
Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells.
Topics: Acridine Orange; Apoptosis; Bromodeoxyuridine; Cell Line, Tumor; Cell Proliferation; Comet Assay; Di | 2009 |
Effects of ascorbic acid and β-carotene on HepG2 human hepatocellular carcinoma cell line.
Topics: Acridine Orange; Apoptosis; Ascorbic Acid; beta Carotene; Carcinoma, Hepatocellular; Cell Line, Tumo | 2011 |
Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro.
Topics: Acridine Orange; Apoptosis; Cell Death; Cell Survival; Cells, Cultured; Comet Assay; Composite Resin | 2011 |
Caspofungin kills Candida albicans by causing both cellular apoptosis and necrosis.
Topics: Annexin A5; Antifungal Agents; Apoptosis; Candida albicans; Caspofungin; Cell Membrane; Colony Count | 2013 |
Cytotoxicity of indocyanine green on retinal pigment epithelium: implications for macular hole surgery.
Topics: Cell Death; Cells, Cultured; Coloring Agents; Dose-Response Relationship, Drug; Ethidium; Fluorescei | 2003 |
Mechanisms of coxsackievirus B5 mediated beta-cell death depend on the multiplicity of infection.
Topics: Animals; Apoptosis; Benzimidazoles; Cell Death; Cell Line, Tumor; Cell Nucleus; Cells, Cultured; DNA | 2004 |
DFNA5: hearing impairment exon instead of hearing impairment gene?
Topics: Animals; Base Sequence; Benzimidazoles; Cell Death; Cell Line; Chlorocebus aethiops; COS Cells; Ethi | 2004 |
Necrotic cell death of ovarian adenocarcinoma caused by seminal plasma.
Topics: Acridine Orange; Adenocarcinoma; Animals; Cell Division; Cell Line, Tumor; DNA Fragmentation; Epithe | 2004 |
Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP.
Topics: Acridine Orange; Antineoplastic Agents; Apoptosis; Cell Division; Cell Line, Tumor; Cell Survival; C | 2004 |
Variation in human islet viability based on different membrane integrity stains.
Topics: Cell Membrane; Cell Survival; Cell Transplantation; Collagenases; Coloring Agents; Ethidium; Fluores | 2004 |
A simple technique for quantifying apoptosis in 96-well plates.
Topics: Acridine Orange; Annexin A5; Antineoplastic Agents, Phytogenic; Apoptosis; Biotechnology; Camptothec | 2005 |
A role for mitogen-activated protein kinase(Erk1/2) activation and non-selective pore formation in P2X7 receptor-mediated thymocyte death.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Apoptosis; Blotting, Western; Calcium; C | 2005 |
Strong electronic charge as an important factor for anticancer activity of chitooligosaccharides (COS).
Topics: Acridine Orange; Antineoplastic Agents; Cell Line, Tumor; Cell Survival; Chitosan; DNA Fragmentation | 2006 |
Protective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair-deficient (xrs5) CHO cells.
Topics: Acridine Orange; Animals; Antigens; Antimutagenic Agents; Apoptosis; beta-Glucans; Cell Survival; CH | 2007 |
A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer.
Topics: Animals; Apoptosis; Cell Count; Cell Survival; Cells, Cultured; Ethidium; Kidney Cortex Necrosis; Ki | 2007 |
A fluorescence vital assay for the recognition and quantification of excitotoxic cell death by necrosis and apoptosis using confocal microscopy on neurons in culture.
Topics: Analysis of Variance; Animals; Apoptosis; Cell Death; Cell Survival; Cells, Cultured; Cerebral Corte | 2007 |
In situ detection of cell death in articular cartilage.
Topics: Animals; Cartilage, Articular; Cattle; Cell Death; Chondrocytes; Cytological Techniques; Ethidium; F | 2007 |
Extracellular calcium is not required for gliotoxin or dexamethasone-induced DNA fragmentation: a reappraisal of the use of EGTA.
Topics: Animals; Apoptosis; Calcium; Cells, Cultured; Dexamethasone; DNA; Dose-Response Relationship, Drug; | 1995 |
Detection and quantification of live, apoptotic, and necrotic human peripheral lymphocytes by single-laser flow cytometry.
Topics: Acridine Orange; Apoptosis; Cell Count; Cell Membrane Permeability; Cell Separation; Cell Survival; | 1995 |
Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells.
Topics: Acridine Orange; Animals; Apoptosis; Cattle; Cell Survival; Cells, Cultured; Endothelium, Corneal; E | 1999 |
Critical evaluation of techniques to detect and measure cell death--study in a model of UV radiation of the leukaemic cell line HL60.
Topics: Acridine Orange; Annexin A5; Apoptosis; Azure Stains; Cell Death; Coloring Agents; Electrophoresis, | 1999 |
Blockade of maitotoxin-induced oncotic cell death reveals zeiosis.
Topics: Animals; Apoptosis; Calcium; Cattle; Cell Membrane; Cells, Cultured; Endothelium, Vascular; Enzyme I | 2002 |
[2 forms of cell death: a cytometric and biochemical analysis].
Topics: Animals; Burkitt Lymphoma; Cell Line; Cell Membrane Permeability; Cell Survival; Cells, Cultured; Cr | 1985 |