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ethidium and Necrosis

ethidium has been researched along with Necrosis in 26 studies

Ethidium: A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.
ethidium : The fluorescent compound widely used in experimental cell biology and biochemistry to reveal double-stranded DNA and RNA.

Necrosis: The death of cells in an organ or tissue due to disease, injury or failure of the blood supply.

Research Excerpts

ExcerptRelevanceReference
" Genotoxic and cytotoxic effects of ascorbic acid and β-carotene were evaluated by alkali single cell gel electrophoresis (SCGE), acridine orange/ethidium bromide staining patterns of cells (apoptosis and necrosis) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS)."7.77Effects of ascorbic acid and β-carotene on HepG2 human hepatocellular carcinoma cell line. ( Iseri, OD; Sahin, FI; Yurtcu, E, 2011)
"Ethidium homodimer is a cell-membrane impermeant nuclear fluorochrome that has been widely used to identify necrotic cells in culture."5.34A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer. ( Diamantakos, EA; Edwards, JR; Lamar, PC; Peuler, JD; Prozialeck, WC, 2007)
" Genotoxic and cytotoxic effects of ascorbic acid and β-carotene were evaluated by alkali single cell gel electrophoresis (SCGE), acridine orange/ethidium bromide staining patterns of cells (apoptosis and necrosis) and lipid peroxidation (thiobarbituric acid reactive substances, TBARS)."3.77Effects of ascorbic acid and β-carotene on HepG2 human hepatocellular carcinoma cell line. ( Iseri, OD; Sahin, FI; Yurtcu, E, 2011)
" Fluorescence microscopy using AO/EB (acridine orange/ethidium bromide) staining indicated that KA can induce both apoptosis and necrosis in HL-60 cell cultures, which corroborate the findings with MTT."3.75Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells. ( Bezerra, DP; Câmara, CA; Cavalcanti, BC; Costa-Lotufo, LV; Lima, MA; Magalhães, HI; Moraes, MO; Pessoa, C; Rao, VS; Silveira, ER, 2009)
"An automated fluorescence method for the detection of neuronal cell death by necrosis and apoptosis with sequential acridine orange (AO) and ethidium bromide (EB) staining using confocal microscopy is described."3.74A fluorescence vital assay for the recognition and quantification of excitotoxic cell death by necrosis and apoptosis using confocal microscopy on neurons in culture. ( Antonov, SM; Evstratova, AA; Mironova, EV, 2007)
"The cytotoxic effects of photo-irradiated riboflavin and rose bengal are shown to be mediated by two distinct but parallel pathways, one leading to apoptosis and the other to necrosis."3.70Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells. ( Cho, KS; Choi, JS; Joo, CK; Lee, EH, 1999)
"The reliability of eight distinct methods (Giemsa staining, trypan blue exclusion, acridine orange/ethidium bromide (AO/EB) double staining for fluorescence microscopy and flow cytometry, propidium iodide (PI) staining, annexin V assay, TUNEL assay and DNA ladder) for detection and quantification of cell death (apoptosis and necrosis) was evaluated and compared."3.70Critical evaluation of techniques to detect and measure cell death--study in a model of UV radiation of the leukaemic cell line HL60. ( Guimarães, JE; Leite, M; Leite, PS; Quinta-Costa, M, 1999)
"Ethidium homodimer is a cell-membrane impermeant nuclear fluorochrome that has been widely used to identify necrotic cells in culture."1.34A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer. ( Diamantakos, EA; Edwards, JR; Lamar, PC; Peuler, JD; Prozialeck, WC, 2007)
"We hypothesise that hearing impairment associated with DFNA5 is caused by a highly unusual mechanism, in which skipping of one specific exon leads to disease that is not caused by other mutations in this gene."1.32DFNA5: hearing impairment exon instead of hearing impairment gene? ( Smith, RJ; Thys, S; Timmermans, JP; Van Bockstaele, DR; Van Camp, G; Van Laer, L; Van Tendeloo, VF; Vrijens, K, 2004)

Research

Studies (26)

TimeframeStudies, this research(%)All Research%
pre-19901 (3.85)18.7374
1990's4 (15.38)18.2507
2000's17 (65.38)29.6817
2010's3 (11.54)24.3611
2020's1 (3.85)2.80

Authors

AuthorsStudies
Hosseini, A1
Shetab-Boushehri, SM1
Shetab-Boushehri, SV1
Tomczyk, M1
Drozdowska, D1
Bielawska, A1
Bielawski, K1
Gudej, J1
Tapajós, EC1
Longo, JP1
Simioni, AR1
Lacava, ZG1
Santos, MF1
Morais, PC1
Tedesco, AC1
Azevedo, RB1
Cavalcanti, BC1
Bezerra, DP1
Magalhães, HI1
Moraes, MO1
Lima, MA1
Silveira, ER1
Câmara, CA1
Rao, VS1
Pessoa, C1
Costa-Lotufo, LV1
Yurtcu, E1
Iseri, OD1
Sahin, FI1
Baraba, A1
Zelježić, D1
Kopjar, N1
Mladinić, M1
Anić, I1
Miletić, I1
Hao, B1
Cheng, S1
Clancy, CJ1
Nguyen, MH1
Ho, JD1
Tsai, RJ1
Chen, SN1
Chen, HC1
Rasilainen, S1
Ylipaasto, P1
Roivainen, M1
Lapatto, R1
Hovi, T1
Otonkoski, T1
Van Laer, L1
Vrijens, K1
Thys, S1
Van Tendeloo, VF1
Smith, RJ1
Van Bockstaele, DR1
Timmermans, JP1
Van Camp, G1
Park, LO1
Yoon, JH1
Kim, JD1
Seo, JT1
Bae, SN1
Kosmider, B1
Zyner, E1
Osiecka, R1
Ochocki, J1
Barnett, MJ1
McGhee-Wilson, D1
Shapiro, AM1
Lakey, JR1
Ribble, D1
Goldstein, NB1
Norris, DA1
Shellman, YG1
Auger, R1
Motta, I1
Benihoud, K1
Ojcius, DM1
Kanellopoulos, JM1
Huang, R1
Mendis, E1
Rajapakse, N1
Kim, SK1
Oliveira, RJ1
Matuo, R1
da Silva, AF1
Matiazi, HJ1
Mantovani, MS1
Ribeiro, LR1
Edwards, JR1
Diamantakos, EA1
Peuler, JD1
Lamar, PC1
Prozialeck, WC1
Mironova, EV1
Evstratova, AA1
Antonov, SM1
Redman, SN1
Khan, IM1
Tew, SR1
Archer, CW1
Waring, P1
Sjaarda, A1
Liegler, TJ1
Hyun, W1
Yen, TS1
Stites, DP1
Cho, KS1
Lee, EH1
Choi, JS1
Joo, CK1
Leite, M1
Quinta-Costa, M1
Leite, PS1
Guimarães, JE1
Estacion, M1
Schilling, WP1
Afanas'ev, VN1
Korol', BA1
Mantsygin, IuA1
Nelipovich, PA1
Pechatnikov, VA1

Other Studies

26 other studies available for ethidium and Necrosis

ArticleYear
Evaluation of Cytotoxic, Necrotic, Apoptotic, and Autophagic Effects of Methamphetamine and 3,4-Methylenedioxymethamphetamine on U-87 MG (Glial) and B104-1-1 (Neuronal) Cell Lines.
    Neurotoxicity research, 2022, Volume: 40, Issue:5

    Topics: Acridine Orange; Cell Line; Central Nervous System Stimulants; Designer Drugs; Ethidium; Humans; Met

2022
Human DNA topoisomerase inhibitors from Potentilla argentea and their cytotoxic effect against MCF-7.
    Die Pharmazie, 2008, Volume: 63, Issue:5

    Topics: Antineoplastic Agents, Phytogenic; Apoptosis; Benzopyrans; Camptothecin; Cell Line, Tumor; DNA; DNA,

2008
In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine.
    Oral oncology, 2008, Volume: 44, Issue:11

    Topics: Acridine Orange; Cell Survival; Coloring Agents; DNA Fragmentation; Ethidium; Fluorescent Dyes; Huma

2008
Kauren-19-oic acid induces DNA damage followed by apoptosis in human leukemia cells.
    Journal of applied toxicology : JAT, 2009, Volume: 29, Issue:7

    Topics: Acridine Orange; Apoptosis; Bromodeoxyuridine; Cell Line, Tumor; Cell Proliferation; Comet Assay; Di

2009
Effects of ascorbic acid and β-carotene on HepG2 human hepatocellular carcinoma cell line.
    Molecular biology reports, 2011, Volume: 38, Issue:7

    Topics: Acridine Orange; Apoptosis; Ascorbic Acid; beta Carotene; Carcinoma, Hepatocellular; Cell Line, Tumo

2011
Evaluation of cytotoxic and genotoxic effects of two resin-based root-canal sealers and their components on human leucocytes in vitro.
    International endodontic journal, 2011, Volume: 44, Issue:7

    Topics: Acridine Orange; Apoptosis; Cell Death; Cell Survival; Cells, Cultured; Comet Assay; Composite Resin

2011
Caspofungin kills Candida albicans by causing both cellular apoptosis and necrosis.
    Antimicrobial agents and chemotherapy, 2013, Volume: 57, Issue:1

    Topics: Annexin A5; Antifungal Agents; Apoptosis; Candida albicans; Caspofungin; Cell Membrane; Colony Count

2013
Cytotoxicity of indocyanine green on retinal pigment epithelium: implications for macular hole surgery.
    Archives of ophthalmology (Chicago, Ill. : 1960), 2003, Volume: 121, Issue:10

    Topics: Cell Death; Cells, Cultured; Coloring Agents; Dose-Response Relationship, Drug; Ethidium; Fluorescei

2003
Mechanisms of coxsackievirus B5 mediated beta-cell death depend on the multiplicity of infection.
    Journal of medical virology, 2004, Volume: 72, Issue:4

    Topics: Animals; Apoptosis; Benzimidazoles; Cell Death; Cell Line, Tumor; Cell Nucleus; Cells, Cultured; DNA

2004
DFNA5: hearing impairment exon instead of hearing impairment gene?
    Journal of medical genetics, 2004, Volume: 41, Issue:6

    Topics: Animals; Base Sequence; Benzimidazoles; Cell Death; Cell Line; Chlorocebus aethiops; COS Cells; Ethi

2004
Necrotic cell death of ovarian adenocarcinoma caused by seminal plasma.
    Gynecologic oncology, 2004, Volume: 93, Issue:3

    Topics: Acridine Orange; Adenocarcinoma; Animals; Cell Division; Cell Line, Tumor; DNA Fragmentation; Epithe

2004
Induction of apoptosis and necrosis in A549 cells by the cis-Pt(II) complex of 3-aminoflavone in comparison with cis-DDP.
    Mutation research, 2004, Sep-12, Volume: 563, Issue:1

    Topics: Acridine Orange; Antineoplastic Agents; Apoptosis; Cell Division; Cell Line, Tumor; Cell Survival; C

2004
Variation in human islet viability based on different membrane integrity stains.
    Cell transplantation, 2004, Volume: 13, Issue:5

    Topics: Cell Membrane; Cell Survival; Cell Transplantation; Collagenases; Coloring Agents; Ethidium; Fluores

2004
A simple technique for quantifying apoptosis in 96-well plates.
    BMC biotechnology, 2005, May-10, Volume: 5

    Topics: Acridine Orange; Annexin A5; Antineoplastic Agents, Phytogenic; Apoptosis; Biotechnology; Camptothec

2005
A role for mitogen-activated protein kinase(Erk1/2) activation and non-selective pore formation in P2X7 receptor-mediated thymocyte death.
    The Journal of biological chemistry, 2005, Jul-29, Volume: 280, Issue:30

    Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; Apoptosis; Blotting, Western; Calcium; C

2005
Strong electronic charge as an important factor for anticancer activity of chitooligosaccharides (COS).
    Life sciences, 2006, Apr-11, Volume: 78, Issue:20

    Topics: Acridine Orange; Antineoplastic Agents; Cell Line, Tumor; Cell Survival; Chitosan; DNA Fragmentation

2006
Protective effect of beta-glucan extracted from Saccharomyces cerevisiae, against DNA damage and cytotoxicity in wild-type (k1) and repair-deficient (xrs5) CHO cells.
    Toxicology in vitro : an international journal published in association with BIBRA, 2007, Volume: 21, Issue:1

    Topics: Acridine Orange; Animals; Antigens; Antimutagenic Agents; Apoptosis; beta-Glucans; Cell Survival; CH

2007
A novel method for the evaluation of proximal tubule epithelial cellular necrosis in the intact rat kidney using ethidium homodimer.
    BMC physiology, 2007, Feb-23, Volume: 7

    Topics: Animals; Apoptosis; Cell Count; Cell Survival; Cells, Cultured; Ethidium; Kidney Cortex Necrosis; Ki

2007
A fluorescence vital assay for the recognition and quantification of excitotoxic cell death by necrosis and apoptosis using confocal microscopy on neurons in culture.
    Journal of neuroscience methods, 2007, Jun-15, Volume: 163, Issue:1

    Topics: Analysis of Variance; Animals; Apoptosis; Cell Death; Cell Survival; Cells, Cultured; Cerebral Corte

2007
In situ detection of cell death in articular cartilage.
    Methods in molecular medicine, 2007, Volume: 135

    Topics: Animals; Cartilage, Articular; Cattle; Cell Death; Chondrocytes; Cytological Techniques; Ethidium; F

2007
Extracellular calcium is not required for gliotoxin or dexamethasone-induced DNA fragmentation: a reappraisal of the use of EGTA.
    International journal of immunopharmacology, 1995, Volume: 17, Issue:5

    Topics: Animals; Apoptosis; Calcium; Cells, Cultured; Dexamethasone; DNA; Dose-Response Relationship, Drug;

1995
Detection and quantification of live, apoptotic, and necrotic human peripheral lymphocytes by single-laser flow cytometry.
    Clinical and diagnostic laboratory immunology, 1995, Volume: 2, Issue:3

    Topics: Acridine Orange; Apoptosis; Cell Count; Cell Membrane Permeability; Cell Separation; Cell Survival;

1995
Reactive oxygen species-induced apoptosis and necrosis in bovine corneal endothelial cells.
    Investigative ophthalmology & visual science, 1999, Volume: 40, Issue:5

    Topics: Acridine Orange; Animals; Apoptosis; Cattle; Cell Survival; Cells, Cultured; Endothelium, Corneal; E

1999
Critical evaluation of techniques to detect and measure cell death--study in a model of UV radiation of the leukaemic cell line HL60.
    Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology, 1999, Volume: 19, Issue:3-4

    Topics: Acridine Orange; Annexin A5; Apoptosis; Azure Stains; Cell Death; Coloring Agents; Electrophoresis,

1999
Blockade of maitotoxin-induced oncotic cell death reveals zeiosis.
    BMC physiology, 2002, Volume: 2

    Topics: Animals; Apoptosis; Calcium; Cattle; Cell Membrane; Cells, Cultured; Endothelium, Vascular; Enzyme I

2002
[2 forms of cell death: a cytometric and biochemical analysis].
    Doklady Akademii nauk SSSR, 1985, Volume: 285, Issue:2

    Topics: Animals; Burkitt Lymphoma; Cell Line; Cell Membrane Permeability; Cell Survival; Cells, Cultured; Cr

1985
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