estrone-sulfate and Uterine-Diseases

Plasma cortisol, oestrone sulphate and progestagens were measured in 22 stressed pregnant mares (gestation length 17-336 days) as indicators of fetal viability. Mares were bled every 12 h from time of admission, and plasma was stored at -70 degrees C until assayed. Four normal mares were bled twice weekly from Day 270 to parturition to provide baseline endocrine data. Cortisol and progestagen concentrations were measured by radioimmunoassay and oestrone sulphate was measured by enzyme immunoassay. Mares were grouped according to clinical diagnosis: surgical colic (Group 1, n = 11), medical colic (Group 2, n = 7), and uterine torsion (Group 3, n = 4). Of the 16 mares in Groups 1 and 2 that survived to discharge, 12 mares foaled normally and 4 aborted, 3 during hospitalization. Following surgical treatment of uterine torsion, 2 mares aborted and 2 mares carried foals to term. Plasma cortisol was greater than 30 ng/ml in 19 of the 22 stressed mares at presentation and was less than 30 ng/ml in normal mares at all collections. Cortisol concentrations remained elevated in mares during post admission complications. The mean cortisol concentration of mares with colic that subsequently aborted was higher at presentation, but not statistically different, than levels of mares that did not abort (135 +/- 35 ng/ml and 83 +/- 19 ng/ml, respectively; mean +/- s.e.m.). Progestagen concentrations in normal mares ranged from 2-25 ng/ml.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Abortion, Veterinary; Animals; Colic; Estrogens, Conjugated (USP); Estrone; Female; Fetal Viability; Gestational Age; Horse Diseases; Horses; Hydrocortisone; Pregnancy; Pregnancy Complications; Progestins; Time Factors; Uterine Diseases

The properties of estrone (E1) and dehydroepiandrosterone (DHEA) sulfatase activities are reported. Endometrial biopsy specimens were obtained using a Novak curette. Cycle stage was assessed from histological dating of endometrium, plasma estradiol and progesterone levels, and patient history. Both sulfatases are membrane-bound enzymes. The optimum pHs in Tris-HCl buffer were 6.5 for E1 sulfatase and 7.3 for DHEA sulfatase. Lowest activities and different optimum pHs were obtained with imidazole, maleate, or acetate buffers. DHEA sulfatase is more sensitive to thermal inactivation than E1 sulfatase. From kinetic studies, apparent Km values of 3.1 microM for E1 sulfatase and 5.7 microM for DHEA sulfatase were calculated. Noncompetitive inhibition of E1 sulfatase by DHEA sulfate and of DHEA sulfatase by E1 sulfate were demonstrated. The effects of inorganic ions and unconjugated steroids were also tested. These results are consistent with two different activities hydrolyzing E1 or DHEA sulfates. Neither activity varies during normal menstrual cycles nor is not correlated to plasma progesterone or 17 beta-estradiol levels. An isolated increase in E1 sulfatase occurred in the proliferative phase of irregular menstrual cycles, postantibiotic-treated salpingitis, or hyperplastic endometrium.

Topics: Dehydroepiandrosterone; Dehydroepiandrosterone Sulfate; Endometrial Hyperplasia; Endometrium; Estrone; Female; Hot Temperature; Humans; Hydrogen-Ion Concentration; Kinetics; Menstruation; Salpingitis; Steryl-Sulfatase; Subcellular Fractions; Sulfatases; Uterine Diseases