estramustine and Uterine-Neoplasms

Topics: Adenocarcinoma; Antineoplastic Agents, Hormonal; Estramustine; Female; Humans; Injections, Intralymphatic; Lymphatic Metastasis; Middle Aged; Uterine Neoplasms

Incubation with estramustine phosphate for 24 h inhibited DNA, RNA and protein synthesis in primary cultures of human kidney, mammary, prostatic, cervical and endometrial carcinoma. Not only the presence, but also the concentration of oestrogen receptors correlated with estramustine phosphate effects on tumour cell proliferation.

Topics: Cell Division; Cytosol; DNA, Neoplasm; Dose-Response Relationship, Drug; Estramustine; Female; Humans; Kidney Neoplasms; Leucine; Male; Prostatic Neoplasms; Protein Biosynthesis; Receptors, Estrogen; RNA, Neoplasm; Thymidine; Tumor Cells, Cultured; Uridine; Uterine Cervical Neoplasms; Uterine Neoplasms

The effects of Estracyt, a Nitrogen mustard (HN2) derivative of estradiol-17 beta versus the free HN2 on cell kinetics of the estrogen receptor positive human endometrial cancer cell line HEC-1 were investigated using flow cytometry. The results were as follows. The cell killing effects of Estracyt existed in a dependency on dose and time, whereas those of HN2 were dependent on dose alone. HN2 at 1 microgram/ml showed a marked increase in S phase and decrease in G0+1 phase. However, with equivalent doses of Estracyt at 10 micrograms/ml, even more remarkable was the accumulation in the G2+M phase. Synchronization at S phase with MTX showed no increase in sensitivity to these drugs on cells in S phase. Based on the above results, it was suggested that the free HN2 had an affect regardless of the cell cycle phase, whereas the effects of Estracyt corresponded to the cell cycle phase and Estracyt might have a fixed population of non-target cells existing in the G0+1 and S phase. Synchronization in the G1 phase with Sodium n-butylate could increase the target effects of Estracyt in S phase.

Topics: Adenocarcinoma; Cell Cycle; DNA, Neoplasm; Estramustine; Female; Flow Cytometry; Humans; In Vitro Techniques; Interphase; Nitrogen Mustard Compounds; Uterine Neoplasms

Topics: Binding, Competitive; Estradiol; Estramustine; Female; Humans; In Vitro Techniques; Kinetics; Neoplasms, Hormone-Dependent; Nitrogen Mustard Compounds; Receptors, Estrogen; Uterine Neoplasms; Uterus

After having succeeded in imaging the prostate with 131I-Estracyt in our former experiments we tried to use the same method for scanning gynecological tumors. In about fifty per cent of the patients (34 cases) there was a convenient accumulation of the labeled estrogen, first of all the adenoid tumors, myomas of uteri and carcinomas can take up this radiopharmacon. Though the number of cases is rather low it is to be concluded that tumors of hormonal dependence can be scanned using estrogen hormon. Further investigations are in progress.

Topics: Estramustine; Female; Genital Neoplasms, Female; Humans; Iodine Radioisotopes; Nitrogen Mustard Compounds; Ovarian Neoplasms; Radionuclide Imaging; Uterine Neoplasms