estradiol-3-benzoate and Sexual-Dysfunction--Physiological

Yougui pill combined with Buzhong Yiqi decoction (YPBYD) is used to relieve sexual dysfunction in clinical practice.. To investigate changes in microbial composition caused by sexual dysfunction and identify dominant bacteria related to YPBYD treatment.. After YPBYD treatment, the levels of E2 and cAMP in OVX rats significantly increased (E2: from 20.45 ± 1.60 ng/L to 24.38 ± 1.70 ng/L; cAMP: from 261.41 ± 9.21 pg/mL to 373.75 ± 17.37 pg/mL). OVX treatment decreased diversity of gut microbiota and YPBYD treatment restored gut microbiota composition. Compared with Sham group, the abundance of. These findings are the first to indicate YPBYD can alleviate female sexual dysfunction by modulating gut microbiota in OVX rats, which will help enhance the understanding on potential mechanism of YPBYD against sexual dysfunction.

Topics: Animals; Cyclic AMP; Drugs, Chinese Herbal; Estradiol; Female; Gastrointestinal Microbiome; Ovariectomy; Rats; Rats, Sprague-Dawley; RNA, Ribosomal, 16S; Sexual Dysfunction, Physiological

These studies were designed to develop a paradigm for the detection of antidepressant-induced sexual dysfunction in female rats. Ovariectomized, Fischer rats were conditioned to nose poke to open a guillotine door to gain access to a sexually active male. To develop the procedure, we examined the acquisition and stability of the response with a 15-s fixed interval, compared rats treated with 10 μg estradiol benzoate and 500 μg progesterone with those that received only estradiol benzoate, and carried out a preliminary analysis of the effects of 5, 10, and 15 mg/kg fluoxetine. We then more fully evaluated the effects of 5 mg/kg fluoxetine. Fluoxetine reduced sexual motivation, as assessed by the number of nose pokes, the number of nose poke episodes, and the latency to approach the male. In addition, changes in the females' sexual motivation were examined before and after ejaculation during the final conditioning trials. The number of nose pokes was reduced and the latency to initiate a new nose poke episode was increased following ejaculation. The robustness of the antidepressant-induced decline in sexual motivation is in marked contrast to the findings with several other animal models for sexual dysfunction and illustrates the usefulness of the operant procedure.

Topics: Animals; Antidepressive Agents; Conditioning, Operant; Disease Models, Animal; Dose-Response Relationship, Drug; Estradiol; Female; Fluoxetine; Hormones; Male; Motivation; Motor Activity; Ovariectomy; Progesterone; Psychological Tests; Rats, Inbred F344; Rats, Sprague-Dawley; Sexual Behavior, Animal; Sexual Dysfunction, Physiological

In the present study, we evaluated the effects of single components of Ferula hermonis extract on female rat sexual behaviour. Ovariectomized rats hormonally primed with estradiol benzoate (1.5 or 10 microg/rat s.c.) and progesterone (500 microg/rat s.c.) were acutely treated by oral gavage with ferutinin, teferin and teferdin (2.5 mg/kg). Thereafter they were tested for: a) partner preference, b) receptivity, c) proceptivity, d) paced mating behaviour. In the partner preference test, the choice of the female for a sexually active male was not influenced by the different treatments. Similarly, during the paced mating test, the contact-return latencies as well as the percentage of exits from the male compartment were not different in control and treated rats. Therefore none of the three compounds showed the capacity to alter sexual motivation. On the other hand, ferutinin, but not teferin and teferdin, significantly inhibited female receptivity. These results suggest a primary role of ferutinin in the impairment of sexual behaviour elicited by F. hermonis extract in hormone primed-female rats.

Topics: Analysis of Variance; Animals; Behavior, Animal; Benzoates; Bridged Bicyclo Compounds; Cycloheptanes; Estradiol; Female; Ferula; Male; Ovariectomy; Rats; Rats, Sprague-Dawley; Sesquiterpenes; Sexual Behavior, Animal; Sexual Dysfunction, Physiological