epiglucan and Wounds--Penetrating

Porous scaffolds composed of gelatin and beta-glucan were prepared using the freeze-drying method. The scaffold had an inter-connected pore structure with average pore size of 90-150 microm. Results for the contact angle and cell attachment revealed that a high gelatin content was suitable for cellular attachment and distribution in two- or three-dimensional fibroblast cultures, because the gelatin had acidic residues, and arginine-glycine-aspartic acid groups. To prepare a stratified wound dressing to mimic the normal human skin, fibroblasts and keratinocyte cells were isolated from a child's foreskin, and were co-cultured in gelatin/beta-glucan scaffolds were cross-linked using 1-ethyl-(3-3-dimethylaminopropyl) carbodiimide hydrochloride. An in vivo study showed that after 1 week, the artificial dermis containing the fibroblasts enhanced the re-epithelialization of a full-thickness skin defect rather than the acellular scaffold.

Topics: Animals; Cell Adhesion; Cell Division; Child; Culture Techniques; Dermis; Extracellular Matrix; Fibroblasts; Freeze Drying; Gelatin; Glucans; Humans; Membranes, Artificial; Mice; Mice, Inbred BALB C; Mice, Nude; Skin, Artificial; Tissue Engineering; Treatment Outcome; Wound Healing; Wounds, Penetrating