epidermal-growth-factor and Retinal-Detachment

Rhegmatogenous retinal detachment (RRD) is caused by one or more full-thickness retinal breaks. The current RRD treatments have several drawbacks. Chitosan is one of the most commonly used natural polymers for wound healing and has been demonstrated to be biodegradable, biocompatible, non-toxic, bioadhesive, and bioactive. This study aimed to determine the reliability and effectiveness of chitosan for sealing retinal breaks in rabbits.. Eighteen blue purple rabbits were randomly divided into three groups: chitosan (n = 6), RRD (n = 6), and control (n = 6). The RRD model was established using vitrectomy, making retinal holes, and subretinal fluid injection in the RRD and chitosan groups. One week after the establishment of the model, chitosan was applied within the range of the holes in the chitosan group, and the vitreous body was filled with perfusion fluid. Except the chitosan treatment, the RRD group underwent the same procedure. Intraocular pressure (IOP) measurement, fundus photography, B-mode ultrasound, optical coherence tomography (OCT), histology, and enzyme linked immunosorbent assay (ELISA) were performed.. Retinas of all eyes in the RRD group were detached, whereas those of all eyes in the chitosan group remained attached. The concentrations of epidermal growth factor (EGF), fibroblast growth factor (FGF)-2, transforming growth factor β (TGF-β), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), and IL-8 in the vitreous fluid of the RRD group were significantly higher than those of the control group (. Chitosan may be a reliable method for sealing retinal breaks. Moreover, chitosan can maintain high levels of growth factors and reduce inflammatory factors in the vitreous, which may reduce and delay the death of retinal cells and help restore visual function after retinal repositioning.

Topics: Animals; Chitosan; Epidermal Growth Factor; Interleukin-6; Interleukin-8; Rabbits; Reproducibility of Results; Retinal Detachment; Retinal Perforations; Retrospective Studies; Transforming Growth Factor beta; Vascular Endothelial Growth Factor A

To investigate the relationships between proliferative vitreoretinopathy (PVR) formation and the levels of human epidermal growth factor (h-EGF) and tumor necrosis factor (TNF) in serum and subretinal fluid (SRF) before and after surgery of retinal detachment with PVR.. Serum and SRF samples of 51 patients with retinal detachment and PVR were collected, and their h-EGF and TNF levels were measured by radioimmunoassays.. In comparison with the control group, the levels of h-EGF and TNF in serum and SRF in retinal detachment with PVR group were higher but not statistically significant (P > 0.05). Their levels in the serum were not related to the severity of the disease, while their levels in SRF increased significantly with the increase of the severity of the disease, and these two factors were positively correlated (P < 0.05). 5 days after the surgery of retinal detachment, the h-EGF and TNF levels in serum increased significantly (P < 0.01) and the levels decreased within 30 days but not to normal.. The h-EGF and TNF in SRF might have a synergic action and affect the formation and development of PVR. The h-EGF and TNF in serum take part in the repair of the operative trauma and inflammatory reaction. However, their high levels could interfere with the effect of operation.

Topics: Adult; Epidermal Growth Factor; Extracellular Space; Exudates and Transudates; Female; Humans; Intraoperative Period; Male; Middle Aged; Retinal Detachment; Tumor Necrosis Factor-alpha; Vitreoretinopathy, Proliferative