epidermal-growth-factor and Lichen-Planus--Oral

Oral lichen planus (OLP) is classified as a potential malignant disorder, and epidermal growth factor (EGF) may play a key role in cancer development. The aim of this study was to compare serum and saliva EGF among patients with OLP and oral squamous cell carcinoma (OSCC). A cross-sectional study was performed on 27 patients with OLP (10 reticular and 17 atrophic-erosive forms), 27 patients with OSCC and 27 healthy control group. The study was conducted at the Cancer Department, Clinic of Oral Medicine, Tehran University of Medical Sciences. The serum and saliva EGF were assayed by ELISA method. Statistical analysis of ANOVA was used. The mean serum EGF in OLP and OSCC patients was significantly lower compared to healthy control group (P<0.05), but no significant difference was observed between OLP and OSCC patients. There was no significant difference in mean salivary EGF among groups. As serum EGF levels appear to be statistically similar in OLP and OSCC, it seems that EGF might play a role in the pathogenesis of OLP and its cancerization.

Topics: Adult; Aged; Aged, 80 and over; Carcinoma, Squamous Cell; Case-Control Studies; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor; Female; Humans; Iran; Lichen Planus, Oral; Male; Middle Aged; Mouth Neoplasms; Saliva

In the present study, we investigate the expression profile of the epidermal growth factor receptor family, which comprises EGFR/ErbB1, HER2/ErbB2, HER3/ErbB3 and HER4/ErbB4 in oral leukoplakia (LP). The expression of four epidermal growth factor receptor (EGFR) family genes and their ligands were measured in LP tissues from 14 patients and compared with levels in 10 patients with oral lichen planus (OLP) and normal oral mucosa (NOM) from 14 healthy donors by real-time polymerase chain reaction (PCR) and immunohistochemistry. Synchronous mRNA coexpression of ErbB1, ErbB2, ErbB3 and ErbB4 was detected in LP lesions. Out of the receptors, only ErbB4 mRNA and protein was more highly expressed in LP compared with NOM tissues. These were strongly expressed by epithelial keratinocytes in LP lesions, as shown by immunohistochemistry. Regarding the ligands, the mRNA of Neuregulin2 and 4 were more highly expressed in OLP compared with NOM tissues. Therefore, enhanced ErbB4 on the keratinocytes and synchronous modulation of EGFR family genes may contribute to the pathogenesis and carcinogenesis of LP.

Topics: Adult; Aged; Amphiregulin; Betacellulin; EGF Family of Proteins; Epidermal Growth Factor; Epiregulin; ErbB Receptors; Female; Gene Expression Profiling; Glycoproteins; Heparin; Heparin-binding EGF-like Growth Factor; Humans; Intercellular Signaling Peptides and Proteins; Keratinocytes; Leukoplakia, Oral; Lichen Planus, Oral; Ligands; Male; Middle Aged; Mouth Mucosa; Nerve Growth Factors; Neuregulins; Real-Time Polymerase Chain Reaction; Receptor, ErbB-2; Receptor, ErbB-3; Receptor, ErbB-4; Receptors, Cell Surface; RNA, Messenger; Transforming Growth Factor alpha; Up-Regulation

This study aimed to investigate the roles of the epidermal growth factor receptor (EGFR) family members and their ligands in oral lichen planus (OLP).. The expressions of 4 EGFR-like receptors and 6 EGF-like ligands were measured in OLP tissues from 10 patients and compared with the levels in normal oral mucosa (NOM) from 10 healthy donors.. Of the receptors, only EGFR mRNA and protein were more highly expressed in OLP compared with NOM tissues. Regarding the ligands, the mRNAs of amphiregulin (AREG), epiregulin (EREG), and heparin-binding EGF-like growth factor (HB-EGF) were more highly expressed in OLP compared with NOM tissues. These ligands were strongly expressed by infiltrating lamina propria lymphocytes as well as epithelial keratinocytes in OLP lesions, as shown by immunohistochemistry.. The enhanced EGFR expression on the keratinocytes in OLP lesions and the up-regulation of EGF-like ligands in keratinocytes and infiltrating mononuclear cells could contribute to the carcinogenesis and pathogenesis of OLP.

Topics: Adult; Aged; Amphiregulin; Betacellulin; CD3 Complex; EGF Family of Proteins; Epidermal Growth Factor; Epiregulin; Epithelial Cells; ErbB Receptors; Female; Glycoproteins; Heparin; Heparin-binding EGF-like Growth Factor; Humans; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; Keratinocytes; Leukocytes, Mononuclear; Lichen Planus, Oral; Lymphocytes; Male; Middle Aged; Mouth Mucosa; Polymerase Chain Reaction; Receptor, ErbB-2; Receptor, ErbB-3; Receptor, ErbB-4; Receptors, Cell Surface; T-Lymphocytes; Transforming Growth Factor alpha; Up-Regulation

To examine the effect of mouthwash Yupingfeng on the level of salivary epidermal growth factor (sEGF) in oral lichen planus (OLP).. The level of sEGF was measured by radioimmunoassay with ligand 125I-EGF. The saliva samples were taken from the normal control group and the OLP patients before and after treatment with Yupingfeng.. The levels of sEGF in OLP patients before treatment with Yupingfeng were (4.09 +/- 3.64) microg/L, which was significantly higher than that in normal control [(2.15 +/- 1.62) microg/L, P = 0.013], and (2.57 +/- 1.19) microg/L after treatment,which was significantly lower than before treatment (P = 0.05).. Yupingfeng can modulate the level of sEGF in OLP patients.

Topics: Adult; Aged; Case-Control Studies; Drugs, Chinese Herbal; Epidermal Growth Factor; Female; Humans; Lichen Planus, Oral; Male; Middle Aged; Saliva

To explore the expression of epidermal growth factor (EGF), epidermal growth factor receptor (EGFR), c-fos and c-jun in oral lichen planus (OLP).. The levels of gene expression of EGF, EGFR, c-fos and c-jun were detected with reverse transcription-polymerase chain reaction (RT-PCR) in 10 cases of erosive OLP, 12 cases of reticular OLP and 9 cases of normal oral mucosa. Protein contents and distribution of EGF, EGFR, C-fos and C-jun were examined with immunohistochemical technique in different tissues.. The endogenous levels of EGFR mRNA and protein in erosive OLP were (55.9 +/- 23.1)% and (71.1 +/- 10.1)%, respectively, and significantly higher than those in reticular OLP and normal oral mucosa. The mRNA contents of c-fos and c-jun were significantly higher in erosive OLP than in normal oral mucosa. The positive cell rates of c-fos and c-jun protein in erosive OLP were 1.3 and 1.5 times of those in normal oral mucosa, respectively (P < 0.05).. The probability of carcinomatous change of erosive OLP might be higher than that of reticular OLP, in which EGF and EGFR might play an important role.

Topics: Adult; Case-Control Studies; Epidermal Growth Factor; ErbB Receptors; Humans; Lichen Planus, Oral; Middle Aged; Mouth Mucosa; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; RNA, Messenger