epidermal-growth-factor and Infertility

Topics: Animals; Antigens, CD; Cell Membrane; Epidermal Growth Factor; Fertilization; Heparin-binding EGF-like Growth Factor; Humans; Infertility; Integrins; Intercellular Signaling Peptides and Proteins; Membrane Fusion; Membrane Glycoproteins; Mice; Mice, Knockout; Protein Binding; Tetraspanin 29

The aims of this study were to evaluate the presence of insulin-like growth factor (IGF)-I, platelet-derived growth factor (PDGF), and epidermal growth factor (EGF) in pre-ovulatory follicular fluid (FF) in patients undergoing ovarian hyperstimulation for intra-cytoplasmic sperm injection (ICSI) treatment, to determine the differences between the concentrations of these cytokines in relation to ovarian stimulation regimens, and to find the relationship between these parameters and estradiol 17-beta, progesterone, and luteinizing hormone (LH) concentration in serum, as well as ICSI outcome.. IGF-I and PDGF were measured in the FF of 85 patients. The IGF-I levels were measured by radioimmunoassay, whereas the concentrations of PDGF and EGF were measured by enzyme-linked immunosorbent assay technique, using commercially available kits.. IGF-I (0.42 +/- 0.09 ng/mL), PDGF (307.3 +/- 274.5 pg/mL), and EDF (8.88 +/- 6.4 pg/mL) were present in pre-ovulatory FF in patients undergoing ovarian hyperstimulation for ICSI treatment. The mean concentration of IGF-I in the follicle-stimulating hormone (FSH) group was significantly higher (P = 0.036) than that found in the human menopausal gonadotrophin (hMG)/FSH group, whereas no significant difference in the mean concentrations of PDGF (P = 0.58) and EGF was shown between all investigated groups.. Controlled ovarian stimulation regimens affect only IGF-I levels in FF and the cytokine concentrations of all investigated groups, in turn, showed no correlation either with steroid hormones in serum or ICSI outcome.

Topics: Adult; Chorionic Gonadotropin; Cytokines; Epidermal Growth Factor; Estradiol; Female; Follicular Fluid; Follicular Phase; Humans; Infertility; Insulin-Like Growth Factor I; Luteinizing Hormone; Male; Ovulation Induction; Platelet-Derived Growth Factor; Pregnancy; Pregnancy Outcome; Progesterone; Sperm Injections, Intracytoplasmic

To determine the levels of epidermal growth factor (EGF) and leukemia inhibitory factor (LIF) in follicular fluid, if any, and to assess the association of these cytokines with the outcome of in vitro fertilization (IVF).. EGF and LIF levels determined by enzyme-linked immunosorbent assay in 60 preovulatory follicular fluids were compared with 25 IVF outcomes.. Immunoreactive EGF and LIF could be detected in human follicular fluid. Levels of these cytokines were similar in FF obtained from follicles that resulted in fertilized oocytes and those that did not. EGF levels were significantly lower in patients establishing a pregnancy as compared to patients achieving no pregnancy (P < .007). LIF levels were similar in both groups of patients.. EGF appears to be associated with IVF outcome.

Topics: Adult; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor; Estradiol; Female; Fertilization in Vitro; Follicular Fluid; Growth Inhibitors; Humans; Infertility; Interleukin-6; Leukemia Inhibitory Factor; Lymphokines; Male; Predictive Value of Tests; Pregnancy; Pregnancy Outcome; Progesterone; Sperm Count; Sperm Motility