epidermal-growth-factor and Hypospadias

To investigate the etiopathogenesis of congenital hypospadias and the regulating effect of exogenous human chorionic gonadotrophin (hCG) on the epidermal growth factor (EGF) in the phallus of hypospadiac mice.. Mouse models of congenital hypospadias were established. Fifty healthy male mice randomly selected as normal controls received intraperitoneal injection of normal saline, and another 50 with hypospadias were equally divided into an experimental control group, intraperitoneally injected with 1 ml normal saline, and 4 hCG dose groups treated by hCG intraperitoneal injection at 50 IU, 100 IU, 150 IU and 200 IU respectively for 7 consecutive days. The concentrations of EGF in the phallus and serum were detected in different groups by ELISA.. The concentrations of EGF in the phallus were significantly lower in the hypospadias rats than in the normal controls (P < 0.05), the 150 IU and 200 IU hCG groups showing significant differences from the 50 IU, 100 IU and experimental control groups (P < 0.05), as the 50 IU, 100 IU and experimental control groups from the normal control (P < 0.05). But no obvious difference was found in EGF concentration in the serum among different groups (P > 0.05).. Nonsteroidal antiandrogen and decreased concentration of EGF in the mouse phallus may be associated with the etiology of hypospadias. And exogenous hCG at 150 IU or 200 IU can increase the concentration of EGF in the phallus of mice with congenital hypospadias.

Topics: Animals; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Epidermal Growth Factor; Female; Hypospadias; Male; Mice; Mice, Inbred Strains; Penis; Pregnancy; Skin

To investigate the impact of hCG on the epidermal growth factor (EGF) in the phallic tissues of hypospadiac mice.. The Kunming mouse model of congenital hypospadias was established. As controls, 10 three-week-old normal male mice and 10 3-week-old congenital hypospadiac mice were injected with isometric sodium chloride intraperitoneally. Another 40 mice with hypospadias were divided into 4 groups and injected intraperitoneally with 50 IU, 100 IU, 150 IU and 200 IU of hCG respectively per day for 7 days. Then the concentration of ECG in the phallus and in the serum were assayed by ELISA.. The EGF level in the phallus of the hypospadiac mice was much lower than that of the controls statistically (P < 0. 05). After the injection of exogenous hCG, the concentration of ECG increased with the dose of hCG, 50 IU (59.57 +/- 22.16) pg/ml, 100 IU (57.97 +/- 9.59) pg/ml, 150 IU (73.02 +/- 31.35) pg/ml and 200 IU (92.45 +/- 35.74) pg/ml. The concentration of ECG showed no significant difference between the control group and the 150 IU and 2000 IU groups (P > 0.05).. Decreased concentration of EGF in the mouse phallus may be associated with the pathogeny of hypospadias. A proper dose of exogenous hCG may increase the concentration of EGF in the mouse phallus.

Topics: Animals; Chorionic Gonadotropin; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Epidermal Growth Factor; Hypospadias; Injections, Intraperitoneal; Male; Mice; Penis

To determine whether alterations in epidermal growth factor (EGF), which plays a major role both in the masculinization of the genitourinary tract and in wound healing, or EGF receptor (EGFR) expression are associated with hypospadias.. The prepuces from 22 children undergoing circumcision (median age 27 months, range 5-87) and skin biopsies from 16 children undergoing surgery for hypospadias (median age 12 months, range 3-57) were studied. Fourteen patients had distal, one had mid-shaft and one had penoscrotal hypospadias. Immunoperoxidase staining was carried out for EGF and EGFR and the density of the staining quantified using image cytometry by an operator unaware of the diagnosis.. The median EGF density in foreskins obtained by circumcision was 75% (mean 73, SE 2.1) compared with 54% (mean 52, SE 3.9) for tissue samples from patients with hypospadias (P < 0.001); the mean EGFR was similar in both materials.. The mean EGF value was deficient in skin adjacent to hypospadias defects compared to normal phallic skin, suggesting that the inadequate expression of EGF may be related to the aetiology of hypospadias and to possible wound complications following surgery for hypospadias.

Topics: Child; Child, Preschool; Epidermal Growth Factor; ErbB Receptors; Humans; Hypospadias; Immunohistochemistry; Infant; Male