epidermal-growth-factor and Hearing-Loss--Sensorineural

Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In this study, we determined whether DPSCs could differentiate into cochlear hair cell in vitro.. DPSCs derived from human third molar dental pulp were induced into NSCs using a medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) for 7 days, and then into cochlear hair cell using a medium containing EGF and IGF-1 for the next 14 days. We used the neuroepithelial protein marker nestin and cochlear hair cell marker myosin VIIa as the markers for cells differentiation. Cells expressing the positive markers under the microscope were confirmed to have differentiated into cochlear hair cell.. DPSCs were successfully induced to differentiate into NSCs, with mean 24% nestin-positive cells. We found that DPSC-derived NSCs have a great capacity in differentiating into inner ear hair cell-like cells with an average of 81% cells presenting myosin VIIa. Thus, DPSCs have high potential to serve as a good resource for SNHL treatment.. We found the high potential of DPSCs to differentiate into NSC. The ability of DPSCs in differentiating into neural lineage cell made them a good candidate for regenerative therapy in neural diseases, such as SNHL.

Topics: Cell Differentiation; Dental Pulp; Epidermal Growth Factor; Hair Cells, Auditory; Hearing Loss, Sensorineural; Humans; Stem Cells

In this study, we attempted to differentiated human bone marrow-derived mesenchymal stem cells (hBMSCs) to auditory hair cells using growth factors.. Retinoic acid (RA), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) were added to hBMSCs cell culture medium. The cells were evaluated morphologically and the expression of SOX2, POU4F3, MYO7A, and Calretinin at mRNA level and ATOH1 mRNA and protein expression.. After treatment with the growth factors, the morphology of the cells did not change, but evaluation of gene expression at the mRNA level increased the expression of the ATOH1, SOX2, and POU4F3 markers. Growth factors increased the expression of ATOH1 at the protein level. The expression of calretinin showed decreased and MYO7A no significant change in expression.. hBMSCs have the potential to differentiate to hair cell-like using the RA, bFGF, and EGF.

Topics: Basic Helix-Loop-Helix Transcription Factors; Cell Differentiation; Cell Proliferation; Cell- and Tissue-Based Therapy; Cells, Cultured; Epidermal Growth Factor; Fibroblast Growth Factor 2; Gene Expression Regulation; Hair Cells, Auditory; Hearing Loss, Sensorineural; Humans; Mesenchymal Stem Cells; Real-Time Polymerase Chain Reaction; RNA, Messenger; Tretinoin

Usher syndrome type IIa (OMIM 276901), an autosomal recessive disorder characterized by moderate to severe sensorineural hearing loss and progressive retinitis pigmentosa, maps to the long arm of human chromosome 1q41 between markers AFM268ZD1 and AFM144XF2. Three biologically important mutations in Usher syndrome type IIa patients were identified in a gene (USH2A) isolated from this critical region. The USH2A gene encodes a protein with a predicted size of 171.5 kilodaltons that has laminin epidermal growth factor and fibronectin type III motifs; these motifs are most commonly observed in proteins comprising components of the basal lamina and extracellular matrixes and in cell adhesion molecules.

Topics: Amino Acid Sequence; Animals; Cell Adhesion Molecules; Chromosome Mapping; Chromosomes, Human, Pair 1; Cochlea; Epidermal Growth Factor; Extracellular Matrix Proteins; Female; Fibronectins; Frameshift Mutation; Gene Expression; Genes, Recessive; Glycosylation; Hearing Loss, Sensorineural; Humans; Laminin; Male; Molecular Sequence Data; Pedigree; Retina; Retinitis Pigmentosa; Syndrome; Tumor Cells, Cultured