epidermal-growth-factor and Foreign-Body-Reaction

The analysis of the cellular soft tissue response has traditionally focused on using highly skilled human assessment to determine the severity or presence of a particular type of response. This has taken the form of many different assessment criteria, some even attempting some form of 'quantitative' grading index. With the complexity of this response and the availability of high performance computer systems it is not sufficient and no longer necessary to describe a tissue response subjectively. The soft tissue response to a number of implanted materials has been analysed using a combination of immunohistochemistry and image analysis to reproducibly stain and count the cells involved in a soft tissue response to implanted materials, as well as measure their position relative to the implanted material. The combination of cell count and pattern of cell distribution revealed that although the magnitude of the cellular responses changes with respect to time the position of cells with respect to an implanted material does not change significantly over time. The position of cells is controlled more by the type of implant material and the change in subject the material is implanted in.

Topics: Animals; Biocompatible Materials; Drug Delivery Systems; Epidermal Growth Factor; Fibroblast Growth Factors; Foreign-Body Reaction; Hydrogel, Polyethylene Glycol Dimethacrylate; Image Processing, Computer-Assisted; Models, Biological; Polyethylene Glycols; Polymers; Polyurethanes

The effects of locally administered low-dose epidermal growth factor in a steroid-inhibited wound healing were investigated in a rat model.. Long-acting release of epidermal growth factor was enabled using microspheres embedded in gelatin sponge. Study groups consisted of 60 rats with 10 in each: colonic anastomosis only (C), plus pure gelatin sponge (CG), plus epidermal growth factor loaded sponge (CE), colonic anastomosis and steroid (S), plus gelatine sponge (SG), and plus epidermal growth factor-loaded gelatine sponge (SE) groups. Bursting pressure and wound hydroxy-proline content were measured. Bursting sites were recorded. Collagen deposits, inflammation, and foreign body reactions were evaluated.. Bursting pressure and hydroxy-proline contents were found lowest in the S and highest in the CE groups (P <.01). There was almost no difference between C and SE groups. Bursts were encountered in peri-anastomotic normal colon sites in the nonsteroid-treated C, CG, and CE groups. They were noted overwhelmingly at the anastomosis in steroid-inhibited S, SG, and SE groups. Histopathology results showed a standstill at the inflammatory phase of healing in S and SG groups. The best healing was observed in the CE group. Degree of collagen accumulation was well correlated with bursting pressure and hydroxy-proline content data with a negligible foreign body reaction to gelatine sponge.. Continuous local epidermal growth factor administration by microspheres in gelatin increases wound collagen and further enhances healing in colonic anastomoses even with steroid inhibition.

Topics: Anastomosis, Surgical; Animals; Colitis, Ulcerative; Collagen; Colon; Dexamethasone; Drug Evaluation, Preclinical; Drug Implants; Epidermal Growth Factor; Female; Foreign-Body Reaction; Gelatin; Hydroxyproline; Microspheres; Postoperative Complications; Pressure; Rats; Rats, Sprague-Dawley; Surgical Sponges; Surgical Wound Dehiscence; Suture Techniques; Tensile Strength; Wound Healing

Topics: Animals; Cattle; Cells, Cultured; Collagen; Epidermal Growth Factor; Fibroblast Growth Factors; Foreign-Body Reaction; Granulation Tissue; Models, Biological; Phorbol Esters; Rats; Stimulation, Chemical; Wound Healing