epidermal-growth-factor and Focal-Nodular-Hyperplasia

The purpose of this study was to evaluate the gene expression of growth factors and growth factor receptors of primary hepatic masses, including hepatocellular carcinoma (HCC) and nodular hyperplasia (NH), in dogs. Quantitative real-time reverse transcriptase-polymerase chain reaction was performed to measure the expression of 18 genes in 18 HCCs, 10 NHs, 11 surrounding non-cancerous liver tissues and 4 healthy control liver tissues. Platelet-derived growth factor-B (PDGF-B), transforming growth factor-α, epidermal growth factor receptor, epidermal growth factor and hepatocyte growth factor were found to be differentially expressed in HCC compared with NH and the surrounding non-cancerous and healthy control liver tissues. PDGF-B is suggested to have the potential to become a valuable ancillary target for the treatment of canine HCC.

Topics: Animals; Carcinoma, Hepatocellular; DNA Primers; Dog Diseases; Dogs; Electrophoresis, Agar Gel; Epidermal Growth Factor; ErbB Receptors; Focal Nodular Hyperplasia; Gene Expression Regulation, Neoplastic; Hepatocyte Growth Factor; Intercellular Signaling Peptides and Proteins; Liver Neoplasms; Proto-Oncogene Proteins c-sis; Real-Time Polymerase Chain Reaction; Receptors, Growth Factor; Reverse Transcriptase Polymerase Chain Reaction; Transforming Growth Factor alpha