enprostil and Necrosis

Female mice, eight weeks old, were injected with carbon tetrachloride (CCl4) (10 mg subcutaneously). Groups of mice (n = 10-30) were then injected with enprostil (E) 2, 20 or 50 micrograms/kg body weight (bw) intraperitoneally 15 min and two h after, or E 100 micrograms/kg bw two h after the CCl4 injection. The mice were killed after 24, 48 or 72 h. Plasma activity concentrations of alanine aminotransferase (ALAT) were determined in blood specimens from the iliac veins. The extent of liver cell necrosis in histological sections was recorded on a 100 mm Visual Analogue Scale (VAS) and measured using the electronic Mini Mop method. In the group given the highest single dose of E (100 micrograms/kg) a significant lowering of the CCl4-induced liver cell necrosis was found after 24 h. No significant differences were found after 48 and 72 h. In the other groups injected with lower doses of E after CCl4, no significant differences were found compared to groups injected with CCl4 alone.

Topics: Alanine Transaminase; Animals; Carbon Tetrachloride; Carbon Tetrachloride Poisoning; Dinoprostone; Enprostil; Female; Liver; Mice; Mice, Inbred Strains; Necrosis

Six percent hydrogen peroxide (H2O2) was used as a generator of the *OH free radical, and as an aggressor of gastric mucosa, in 100 Wistar rats. The mucosal cytoprotector effect of sucralfate, misoprostol, enprostil, cimetidine, ranitidine, famotidine and 10% aluminum sulphate yielded almost complete macroscopic and histological protection to the gastric mucosa. Misoprostol or enprostil gave partial protection whereas the H2 blockers aggravated the gastric necrotic lesions produced by the H2O2. We conclude that sucralfate is a true anti-oxidant that protects the gastric mucosa through its aluminum and sulphydril components, the increment of gastric mucins and endogenous PGs.

Topics: Alum Compounds; Animals; Antioxidants; Cimetidine; Enprostil; Famotidine; Female; Free Radicals; Gastric Mucosa; Glycosaminoglycans; Hydrogen Peroxide; Hydroxides; Male; Misoprostol; Necrosis; Ranitidine; Rats; Rats, Inbred Strains; Sucralfate

Topics: Acetaminophen; Aluminum Hydroxide; Animals; Bicarbonates; Drug Evaluation, Preclinical; Enprostil; Ethanol; Gastric Juice; Gastric Mucosa; Indomethacin; Magnesium; Necrosis; Prostaglandins; Prostaglandins E, Synthetic; Rats; Rats, Inbred Strains; Sodium; Sodium Bicarbonate