enkephalin-leu--ala(2)-arg(6)- and Necrosis

Topics: Animals; Drug Evaluation, Preclinical; Enkephalin, Leucine-2-Alanine; Male; Multiple Trauma; Necrosis; Rabbits; Radiation Injuries, Experimental; Time Factors; Wound Healing; Wounds, Gunshot

The article deals with clinical experience in early diagnosis of pancreatonecrosis in which enzymological methods of examination acquire importance. The most informative test in the diagnosis of fatty pancreatonecrosis is the increase of the pancreatic lipase content in blood serum, particularly in stimulation of pancreatic secretion by pancreozymin. A new Soviet-produced peptide agent--dalargin was included in the complex drug treatment of fatty pancreatonecrosis in 74 patients. With the use of dalargin as an inhibitor of the activity of pancreatic enzymes and kinins in the treatment of fatty pancreatonecrosis a marked positive effect was produced. The mortality in fatty pancreatonecrosis was 5.5%.

Topics: Cholecystokinin; Clinical Enzyme Tests; Enkephalin, Leucine-2-Alanine; Humans; Lipase; Necrosis; Pancreas; Pancreatitis

The effect of an opioid hormone (Leu-enkephalin analog Tyr-Dala-Gly-Phe-Leu-Arg) on the healing of myocardial infarction caused by the ligation of the descending branch of the rabbit left coronary artery has been investigated. The peptide was injected intraperitoneally at a dose of 10 micrograms/kg daily. Using light and electron microscopy, the peptide has been shown to exert a pronounced stimulating effect on the healing of the necrotic zone on days 3 and 7 of the experiment. Opioid peptides are suggested to take part in the healing of various disorders.

Topics: Animals; Drug Evaluation, Preclinical; Enkephalin, Leucine; Enkephalin, Leucine-2-Alanine; Male; Microscopy, Electron; Myocardial Infarction; Myocardium; Necrosis; Rabbits; Time Factors; Wound Healing

The role and possible clinical use of regulatory peptides, a new type of regulatory substances, is discussed. Special attention is paid to the opioid peptides and their analogues. The new drug dalargin has been developed on the basis of the endogenous opioid peptide leucine-encephalin. Its action has been studied using experimental models. It has been established that the optimal dose (10 micrograms/kg) of dalargin is effective in preventing ulceration in the cystamine duodenal ulcer rat model and the development of stomach erosive lesions in immobilization stress, reducing the degree of liver degeneration in CCl4 poisoning, etc. With an increase of the dose of dalargin the effect "escapes". Dalargin is primarily bound by delta-receptors, it produces no analgetic effect and does not enter the brain. Dalargin exhibits a pronounced cytoprotective and regeneratory action and may take an important place in treating the internal organ diseases.

Topics: Animals; Carbon Tetrachloride Poisoning; Cells, Cultured; Disease Models, Animal; Dogs; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical; Duodenal Ulcer; Enkephalin, Leucine; Enkephalin, Leucine-2-Alanine; Hemorrhage; Humans; Lymphocytes; Mice; Necrosis; Pancreas; Rats; Receptors, Opioid; Stomach Ulcer