endothelin-1 and Oral-Submucous-Fibrosis

Oral cancer is one of the highly prevalent cancers worldwide being. According to data of GLOBOCAN 2018, the estimated incidence, mortality and 5-year survival rates due to lip, oral cavity and salivary gland cancer in world is (2.0%), (0.5%) and (0.3%) respectively. (Bray, Ferlay and Soerjomataram, 2018). Endothelin-1 (ET-1) is a 21-amino acid peptide; its receptors have been implicated in the growth and progression of both primary and metastatic neoplasms throughout the human body. Studies have shown that ET-1 is expressed in tissue, serum and other body fluids.. To estimate the levels of salivary endothelin-1 in Oral potentially malignant disorders (oral leukoplakia and submucous fibrosis) and oral squamous cell carcinoma.. The study population included 60 subjects and were divided into 4 groups. All patients included in the study are clinically and histopathological diagnosed cases of oral leukoplakia, submucous fibrosis and oral cancer and assessed for salivary ET-1 levels using human ELISA kit. Significant differences between the groups were determined using one-way analysis of variance, LSD and Post HOC, unpaired t test, biserial and spearson's correlation.. The mean levels of salivary Endothelin-1 level in study groups were: 82.78 ± 5.9 pg/mL (OSCC), 65.02 ± 1.8 pg/mL (SMF), 57.76 ± 4.1 pg/mL (LEUKOPLAKIA), 29.72 ± 14.1 pg/mL (CONTROLS). The mean Salivary ET-1 levels among these four groups was compared and the difference was statistically significant (P < 0.001). We also found a significant difference in the means of ET-1 levels among the clinical and histopathological staging of the study groups.. Our results demonstrate potential utility of salivary analysis for ET-1 levels to monitor patients at risk for OSCC. Although provides the basis for a larger prospective study to determine the critical levels of salivary ET-1 necessary to diagnose and monitor OPMD's and its potential to undergo malignant transformation.

Topics: Biomarkers, Tumor; Carcinoma, Squamous Cell; Endothelin-1; Humans; Mouth Neoplasms; Oral Submucous Fibrosis; Prospective Studies

This experiment is carried out to investigate roles of endothelin-1 (ET-1), a potent vasoconstrictor and mitogenic peptide, in fibrosis and collagen production.. 30 cases of oral submucous fibrosis(OSF), 10 cases of oral lichen planus (OLP) and 10 cases of normal control, were studied by means of SABC immunohistochemical method with polyclonal antisera to ET-1 and using image pattern analysis technique.. 1. Oral mucosa from OLP and normal control expressed very little ET-1. By contrast, there was striking expression of ET-1 in oral mucosa from OSF; 2. The cellular content of ET-1 in OSF was significantly higher than that in normal control (P < 0.01), while the cellular content of ET-1 in the early and moderate stages OSF were significantly higher than that in the advanced stage(P < 0.01); 3. The cellular content of ET-1 in the early and moderate stages OSF were significantly higher than that in OLP(P < 0.01); 4. There was a significantly positive correlation between cellular contents of epithelia and interstitial(P < 0.05).. These findings suggest that cell-specific expression of ET-1 may play a role in pathogenesis of OSF.

Topics: Endothelin-1; Humans; Immunohistochemistry; Lichen Planus, Oral; Mouth Mucosa; Oral Submucous Fibrosis

To determine the endothelin-1 (ET-1) immunoreactivity in biopsies from human oral submucous fibrosis (OSF) and to investigate the relation between ET-1 and OSF.. The localization of ET-1 in biopsies from OSF was observed with immunoelectron microscopic techniques.. High-density deposits existed predominantly in the cytoplasm of epithelial cells, fibroblasts and endothelial cells, but not found in the biopsies of normal controls. In the biopsies of OSF, high-density granules were lined on the cell membrane, possibly on the rough endoplasmic reticulum and the mitochondria of the epithelial cells and of the fibroblasts. But a few granules were seen in the cytoplasm of the endothelial cells of microvesseles.. ET-1 is one of the pathogenetic factors of OSF.

Topics: Adult; Biopsy; Endothelin-1; Female; Humans; Male; Microscopy, Immunoelectron; Mouth Mucosa; Oral Submucous Fibrosis