eleostearic-acid and Inflammation

The present work provides first-time empirical and molecular interaction evidence to establish the higher biofunctionality of a therapeutic lipid, α-eleostearic acid (ESA), encapsulated in a novel and thoroughly characterized biocompatible nanoemulsion (NE) system (particle size <200 nm).. A novel methodology was employed to fabricate novel formulations of ESA. Molecular biological tools and assays were used to arrive at definite conclusions.. The proinflammatory profile was found to be significantly mitigated in the hypersensitized rats administered with the ESA-NE formulation more emphatically as compared with ESA-conventional emulsion in both in vivo and ex vivo models.. The novel ESA-NE formulation shows a lot of palpable promise for clinical applications.

Topics: Animals; Cell Cycle; Cells, Cultured; Emulsions; Flow Cytometry; Healthy Volunteers; Humans; Inflammation; Linolenic Acids; Male; Nitric Oxide; Particle Size; Peroxidase; Plant Oils; Rats

The purpose of the present study was to examine the antioxidant activity of two typical oils obtained from two vegetables, bitter gourd seed and snake gourd seed, containing two different isomers of conjugated linolenic acid (CLnA) against oxidative stress induced by sodium arsenite in relation to tissue lipid peroxidation and inflammation. Male albino rats were taken as subject and divided into six groups: Group 1 was control and Group 2 was treated with sodium arsenite (Sa; 10mg/Kg BW); Groups 3-6 were orally treated with different doses of seed oils maintaining definite concentration of CLnA isomers (0.5% and 1.0% of total lipid for each CLnA isomer) along with sodium arsenite. There was significant increase in lipid peroxidation, pro-oxidant enzyme activity and decrease in antioxidant enzyme activity in brain due to Sa administration. Decrease in total protein content was also observed in plasma, liver and brain of Sa treated group. Significant decrease in phospholipid content and increase in total lipid content and cholesterol content were observed in arsenite treated group. There was significant increase in relative organ weight of liver due to Sa administration. Fatty acid profile of liver and brain lipid shows significant (P<0.05) reduction in most of the polyunsaturated fatty acids and increase in arachidonic acid (20:4n-6) (75.23%) due to inflammation after arsenite treatment. Administration of experimental oils made almost complete restoration of those altered parameters. Overall, these two oils were effective in protecting tissue lipid profiles which were altered due to oxidative stress.

Topics: Administration, Oral; alpha-Linolenic Acid; Animals; Anti-Inflammatory Agents; Antioxidants; Arachidonic Acid; Arsenites; Brain; Catalase; Disease Models, Animal; Fatty Acids, Unsaturated; Glutathione Peroxidase; Inflammation; Isomerism; Linolenic Acids; Lipid Peroxidation; Liver; Male; Oxidative Stress; Phospholipids; Plant Oils; Rats; Sodium Compounds; Superoxide Dismutase