elastin and Rift-Valley-Fever

This work analyzes the immunogenicity of six genetically engineered constructs based on elastin-like recombinamers (ELRs) fused to the Gn glycoprotein from Rift Valley fever virus (RVFV). Upon transfection, all constructs showed no effect on cell viability. While fusion constructs including ELR blocks containing hydrophobic amino acids (alanine or isoleucine) did not increase the expression of viral Gn in eukaryotic cells, glutamic acid- or valine-rich fusion proteins showed enhanced expression levels compared with the constructs encoding the viral antigen alone. However,

Topics: Animals; Antibodies, Viral; Elastin; Rift Valley Fever; Rift Valley fever virus; Sheep; Sheep Diseases; Vaccines, DNA; Valine; Viral Vaccines