egg-white and Hemolysis

egg-white has been researched along with Hemolysis* in 3 studies

Other Studies

3 other study(ies) available for egg-white and Hemolysis

ArticleYear
Stable self-assembled nanostructured hen egg white lysozyme exhibits strong anti-proliferative activity against breast cancer cells.
    Colloids and surfaces. B, Biointerfaces, 2015, Jun-01, Volume: 130

    Chemotherapy side effects have long been a matter of great concern. Here we describe a structurally stable self-assembled nanostructured lysozyme (snLYZ) synthesized using a simple desolvation technique that exhibited anticancer activity, as well as excellent hemocompatibility. Field emission scanning electron microscopy; atomic force microscopy and dynamic particle size analyzer were used for analyzing the synthesized snLYZ. The analysis revealed spherical shape with an average size of 300 nm. Circular dichroism and tryptophan fluorescence spectroscopic analysis revealed its gross change in secondary as well as the tertiary level of the structure. snLYZ also demonstrated excellent structural as well as the functional stability of LYZ in a wide range of pH and temperature with a fair level of protection against proteinase K digestion. When applied to MCF-7 breast cancer cells, it exhibited approximately 95% cell death within 24h, involving a reactive oxygen species (ROS) based mechanism, and showed excellent hemocompatibility. Fluorescence microscopy imaging revealed distinct cellular internalization of snLYZ and the formation of cytoplasmic granules, which initiated a cell-killing process through membrane damage. In order to mimic targeted therapy, we tagged folic acid with snLYZ, which further enhanced cytotoxicity against MCF-7 cells. Therefore, this is the first report of its kind where we demonstrated the preparation of a highly stable self-assembled nanostructured lysozyme with a strong anti-proliferative activity against breast cancer cells.

    Topics: 3T3 Cells; Animals; Breast Neoplasms; Cell Line; Cell Line, Tumor; Cell Proliferation; Chickens; Circular Dichroism; Egg White; Enzyme Stability; Female; Hemolysis; Humans; Hydrogen-Ion Concentration; MCF-7 Cells; Mice; Microscopy, Atomic Force; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Muramidase; Nanostructures; Reactive Oxygen Species; Spectrometry, Fluorescence; Temperature

2015
Toxicity of quartz and kaolin towards membranes: effect of lysozyme.
    Archives internationales de physiologie et de biochimie, 1974, Volume: 82, Issue:5

    Topics: Animals; Egg White; Erythrocytes; Hemolysis; Kaolin; Muramidase; Quartz; Sheep; Silicon Dioxide

1974
Complement activity of the serums of inbred strains of rats during egg-white shock.
    Zeitschrift fur Immunitatsforschung, Allergie und klinische Immunologie, 1966, Volume: 130, Issue:2

    Topics: Anaphylaxis; Animals; Colorimetry; Complement System Proteins; Egg White; Guinea Pigs; Hemolysis; Immune Sera; Injections, Intraperitoneal; Male; Rats

1966