egg-white and Deficiency-Diseases

Blood-based indicators of biotin status in humans were shown to be useful tools in several clinical situations, including pregnancy. We previously validated the activity of the biotin-dependent enzyme propionyl-coenzyme A carboxylase (PCC) in lymphocytes as a sensitive and specific blood-based indicator of marginal degrees of biotin deficiency. However, the measurement of PCC activity in population studies presents substantial analytic challenges. 3-Hydroxyisovaleryl carnitine (3HIA-carnitine) increases in response to the decreased activity of the biotin-dependent enzyme methylcrotonyl-coenzyme A carboxylase and might reflect biotin status.. We sought to determine whether the plasma concentration of 3HIA-carnitine increases significantly in marginal biotin deficiency.. We experimentally induced marginal, asymptomatic biotin deficiency in 10 healthy adults (8 women) by having the subjects consume undenatured egg white for 28 d; biotin status was then repleted. Plasma concentrations of 3HIA-carnitine were measured on days 0, 14, 28, 35, and 50 by liquid chromatography-mass spectroscopy.. The mean plasma 3HIA-carnitine concentration increased with depletion (P < 0.0001) and decreased with repletion (P < 0.0001). Plasma 3HIA-carnitine concentrations were greater than the upper limit of normal concentrations in 7 of 10 subjects by day 14 and in 9 of 10 subjects by day 28 and decreased to within normal limits in 9 of 10 subjects by day 50.. These studies provide evidence that 3HIA-carnitine is an early and sensitive indicator of marginal biotin deficiency. The ease of sample collection, small sample volume requirement, and stability of 3HIA-carnitine during storage suggest that plasma 3HIA-carnitine concentration is likely to be a useful indicator of marginal biotin deficiency for larger population studies.

Topics: Adult; Aged; Biomarkers; Biotin; Carbon-Carbon Ligases; Carnitine; Deficiency Diseases; Egg White; Female; Humans; Male; Middle Aged

Topics: Animals; Body Weight; Coturnix; Deficiency Diseases; Dietary Carbohydrates; Dietary Fats; Dietary Proteins; Egg White; Fasting; Female; Glycine max; Japan; Male; Zinc

Topics: Animal Nutritional Physiological Phenomena; Animals; Arginine; Body Weight; Bone and Bones; Bone Diseases, Developmental; Caseins; Chickens; Congenital Abnormalities; Deficiency Diseases; Depression, Chemical; Dietary Proteins; Egg White; Feathers; Female; Glycine max; Hindlimb; Histamine; Histidine; Liver; Male; Plant Proteins; Stimulation, Chemical; Tibia; Zinc

Topics: Amino Acids; Animals; Caseins; Deficiency Diseases; Dietary Proteins; Egg White; Glycine max; Poultry; Zinc

Topics: Body Composition; Body Weight; Bone and Bones; Calcium; Calcium, Dietary; Chemical Phenomena; Chemistry; Deficiency Diseases; Diet; Dietary Proteins; Egg White; Glycine max; Kidney; Lactose; Minerals; Phosphorus; Rats; Research; Zinc