dynorphins and Glioma

To explore the cell mechanism of brain edema and the effect of dynorphin A1-13 on swelling of C6 glioma cells. Water content of cell are studied by using 3-O-methyl-D-glucose.. (1) Glutamate (0.5, 1.0, 10.0 mmol/L) increased the water content of C6 glioma at an hour. (2) Dynorphin A1-13 could significantly decrease the increasing in water content of C6 glioma cells induced by glutamate. (3) nor-BNI, a antagonist, could inhibit the effect of dynorphin A1-13 on water content of swelling C6 glioma cells.. Glutamate could induce the swelling of C6 glioma cells. Dynorphin A1-13 could reduce C6 glioma cells swelling induced by glutamate via kappa opioid receptor.

Topics: Animals; Dynorphins; Glioma; Glutamic Acid; Rats; Tumor Cells, Cultured

Neuroblastoma x glioma hybrid cells (NG 108CC15) and tumors derived thereof were examined for dynorphin- and alpha-neoendorphin-like material. The techniques employed for analyses of opiate-like material were the isolated mouse vas deferens bioassay and gel chromatography and high pressure liquid chromatography in combination with radioimmunoassays. Dynorphin- and alpha-neoendorphin-like material was detected in both the hybrid cells and the corresponding tumors. Immunoreactive dynorphin and alpha-neoendorphin was also in NCB 20 hybrid cells and in tumors thereof assayed. In all samples investigated, the amounts of alpha-neoendorphin-like material was higher than that of dynorphin-like material. The results revealed considerable variability in the amount of dynorphin- and alpha-neoendorphin-activity between particular samples, suggesting the need for studies into the responsible mechanisms.

Topics: alpha-Endorphin; Animals; Biological Assay; Cell Line; Chromatography, Gel; Chromatography, High Pressure Liquid; Dynorphins; Endorphins; Glioma; Hybrid Cells; Male; Mice; Neuroblastoma; Radioimmunoassay; Rats; Vas Deferens