dolichol-monophosphate and Neutropenia

Severe hematopoietic injury in mice was induced by using either 5-fluorouracil, adriamycin, mitomycin C, or vinblastine. Daily subcutaneous administration of purified human recombinant granulocyte colony-stimulating factor (rG-CSF; 0.3-10.0 micrograms/day) markedly accelerated recovery from the drug-induced granulocytopenia in a dose-dependent manner, as reported previously. On the other hand, daily intraperitoneal administration of dolichyl phosphate (Dol-P) also enhanced granulopoiesis to accelerate recovery from granulocytopenia, although the effect of Dol-P was relatively moderate as compared with that of rG-CSF. A synergistic recovery of granulopoiesis was observed when Dol-P was administered together with rG-CSF to the mice treated with anti-cancer drugs. Joint use of Dol-P (1 mg/day) and rG-CSF (0.3 micrograms/day) was as effective as a higher dose of rG-CSF (3 micrograms/day). Joint use of Dol-P (1 mg/day) and rG-CSF (3 micrograms/day) was sometimes more effective.

Topics: Agranulocytosis; Animals; Antineoplastic Agents; Colony-Stimulating Factors; Dolichol Phosphates; Doxorubicin; Drug Administration Schedule; Drug Synergism; Female; Fluorouracil; Granulocyte Colony-Stimulating Factor; Humans; Mice; Neutropenia; Polyisoprenyl Phosphates; Recombinant Proteins; Vinblastine