dizocilpine-maleate and Jaundice

Bilirubin encephalopathy or kernicterus is a potentially serious complication of neonatal hyperbilirubinemia. The mechanism of bilirubin-induced neurotoxicity is not known. Many neurological insults are mediated through NMDA receptor activation.. We assessed the effect of the NMDA channel antagonist, MK-801 on bilirubin neurotoxicity in vivo and in vitro.. Bilirubin toxicity in vitro was assessed using trypan blue staining. Sulfadimethoxine injected (i.p.) jaundiced Gunn rat pups exhibit many neurological sequelae observed in human hyperbilirubinemia. Brainstem auditory-evoked potentials (BAEPs), a noninvasive sensitive tool to assess auditory dysfunction due to bilirubin neurotoxicity, were used to assess neuroprotection with MK-801 (i.p.) in vivo.. In primary cultures of hippocampal neurons, 20 min exposure to 64:32 microM bilirubin:human serum albumin reduced the cell viability by approximately 50% ten hours later. MK-801 treatment did not protect the cells. MK-801 pretreatment doses ranging from 0.1-4.0 mg/kg did not protect against BAEP abnormalities in Gunn rat pups 6 h after sulfadimethoxine injection.. Our findings suggest that bilirubin neurotoxicity is not mediated through NMDA receptor activation.

Topics: Animals; Animals, Newborn; Anti-Infective Agents; Bilirubin; Cell Survival; Disease Models, Animal; Dizocilpine Maleate; Dose-Response Relationship, Drug; Evoked Potentials, Auditory, Brain Stem; Hyperbilirubinemia; Jaundice; Kernicterus; Neurons; Neuroprotective Agents; Rats; Rats, Gunn; Receptors, N-Methyl-D-Aspartate; Sulfadimethoxine