ditaurobilirubin and Cholestasis

ditaurobilirubin has been researched along with Cholestasis* in 1 studies

Other Studies

1 other study(ies) available for ditaurobilirubin and Cholestasis

Article	Year
Measurement of direct bilirubin by use of bilirubin oxidase. Clinical chemistry, 1987, Volume: 33, Issue:8 We developed an enzymatic method for measuring direct-reacting bilirubin (DBIL) in serum. At pH 4.5, bilirubin oxidase (BOX) oxidizes mono-conjugated bilirubin, di-conjugated bilirubin, and most of the delta-bilirubin to biliverdin. The resulting decrease in absorbance at 460 nm is linearly related to the concentration of DBIL in serum. Mean DBIL values in the 51 patients' sera examined by the BOX method and a diazo procedure (Clin Chem 1982;28:2305) were 45.4 and 42.8 mg/L, respectively. For the same samples, mean values for DBIL and conjugated bilirubin by the Kodak "Ektachem" methods were 50.2 and 24.8 mg/L, respectively. Hemoglobin, up to 1.5 g/L, does not interfere. Unconjugated bilirubin reacts negligibly. Day-to-day CVs were 2.2% and 2.4% at DBIL concentrations of 37 and 74 mg/L, respectively. Topics: Bilirubin; Biliverdine; Cholestasis; Diazonium Compounds; Humans; Indicators and Reagents; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Reference Values; Spectrophotometry; Statistics as Topic; Taurine	1987

Article

Year

Measurement of direct bilirubin by use of bilirubin oxidase.

Clinical chemistry, 1987, Volume: 33, Issue:8

We developed an enzymatic method for measuring direct-reacting bilirubin (DBIL) in serum. At pH 4.5, bilirubin oxidase (BOX) oxidizes mono-conjugated bilirubin, di-conjugated bilirubin, and most of the delta-bilirubin to biliverdin. The resulting decrease in absorbance at 460 nm is linearly related to the concentration of DBIL in serum. Mean DBIL values in the 51 patients' sera examined by the BOX method and a diazo procedure (Clin Chem 1982;28:2305) were 45.4 and 42.8 mg/L, respectively. For the same samples, mean values for DBIL and conjugated bilirubin by the Kodak "Ektachem" methods were 50.2 and 24.8 mg/L, respectively. Hemoglobin, up to 1.5 g/L, does not interfere. Unconjugated bilirubin reacts negligibly. Day-to-day CVs were 2.2% and 2.4% at DBIL concentrations of 37 and 74 mg/L, respectively.

Topics: Bilirubin; Biliverdine; Cholestasis; Diazonium Compounds; Humans; Indicators and Reagents; Oxidoreductases; Oxidoreductases Acting on CH-CH Group Donors; Reference Values; Spectrophotometry; Statistics as Topic; Taurine

1987