disilver-oxide and Disease-Models--Animal

To investigate and compare between the effect of both silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) on insulin signaling pathway and insulin sensitivity in experimental diabetes. Preparation of AgNPs and ZnONPs in their solid state were carried out using pullulan (Natural polymer) as both reducing and stabilizing agent. The synthesis of these nanoparticles in a large scale were carried out without using any solvents. The experimental male albino rats received diluted solutions of AgNPs and ZNONPs. After the experimental period, blood was withdrawn; erythrocyte membrane lipids were extracted and fatty acids were determined by HPLC. Oxidant, antioxidant profile and phosphatidylinositol 3-kinase (PI. It was observed that the as synthesized AgNPs and ZnONPs have nearly spherical shape with small size due to the stabilization effect of pullulan as proved by UV-vis spectroscopy (UV-vis), Transmission electron microscy (TEM) and Field emission scanning electron microscopy (FESEM), Zeta potential, Dynamic light scattering (DLS) and X-ray diffraction (XRD) techniques. The average hydrodynamic size of the formed AgNPs was 15 nm which is considered as very small size when compared with that of ZnONPs (above 50 nm). Fasting blood sugar was significantly increased in diabetic group along with elevation of MDA and DNA damage indicating the oxidative properties of streptozotocin. Whereas, the treatment with nanoparticles significantly attenuated these elevations.. AgNPs and ZnONPs represent promising materials in attenuating diabetic complications and insulin resistance in experimental diabetes; no Impressive differences were observed between the effect of ZnONPs and AgNPs in this current research.

Topics: Animals; Cell Membrane; Diabetes Mellitus, Experimental; Disease Models, Animal; Drug Carriers; Injections, Subcutaneous; Insulin; Male; Nanoparticles; Oxides; Particle Size; Rats; Signal Transduction; Silver Compounds; Streptozocin; Surface Properties; Zinc Oxide

Bacterial infection is a serious postoperative complication of joint replacement. To prevent infections related to implantation, we have developed a novel antibacterial coating with Ag-containing hydroxyapatite (Ag-HA). In the present study, we examined the antibacterial activity of Ag-HA implant coatings in the medullary cavity of rat tibiae. Forty 10-week-old rats received implantation of Ag-HA- or HA-coated titanium rods, then were inoculated with ∼1.0 × 10(2) colony-forming units of methicillin-resistant Staphylococcus aureus. Bacterial counts were calculated for rats euthanized at 24, 48, and 72 h postoperatively. Serum levels of Ag (in the Ag-HA group only) were calculated for rats euthanized at 24, 48, 72 h and 4 weeks. Radiographic evaluations of bone infection were also performed at 4 weeks. Tibiae from both groups showing infection were evaluated histologically. Significant differences in bacterial counts were seen at 24, 48, and 72 h. Mean concentrations of Ag in serum peaked about 48 h after implantation, then gradually decreased. Mean radiographic scores for infection were significantly lower with Ag-HA implants than with HA implants. Histological examination showed better results for abscesses, bone resorption, and destruction of cortical bone around Ag-HA-coated implants. These results indicate that Ag-HA coatings may help prevent surgical-site infections associated with joint replacement.

Topics: Abscess; Animals; Anti-Infective Agents; Bone Nails; Coated Materials, Biocompatible; Disease Models, Animal; Hydroxyapatites; Male; Methicillin-Resistant Staphylococcus aureus; Microbial Sensitivity Tests; Microscopy, Electron, Scanning; Osteomyelitis; Oxides; Rats; Rats, Sprague-Dawley; Silver Compounds; Staphylococcal Infections; Surgical Wound Infection; Tibia; Titanium

Adherent bacterial biofilms have been implicated in the irreversible contamination of implanted medical devices. We evaluated the resistance of various tympanostomy (pressure equalization [PE]) tube materials to biofilm formation using an in vivo model. PE tubes of silicone, silver oxide-impregnated silicone, fluoroplastic, silver oxide-impregnated fluoroplastic, and ion-bombarded silicone were inserted into the tympanic membranes of 18 Hartley guinea pigs. Staphylococcus aureus was then inoculated into the middle ears. An additional 8 guinea pigs were used as controls; the PE tubes were inserted without middle ear inoculation. All PE tubes were removed on day 10 and analyzed for bacterial contamination using culture, immunofluorescence, and scanning electron microscopy (SEM). All infected ears developed otitis media with otorrhea, but none of the animal control ears drained. Fluorescence imaging of the animal control tubes showed large cellular components consistent with inflammation. The infected tubes showed heavy DNA fluorescence consistent with bacteria and inflammatory cells. All animal control tubes except the ion-bombarded silicone tubes showed adherent inflammatory film on SEM. Also, all tubes placed in infected ears except the ion-bombarded silicone tubes showed adherent bacterial and inflammatory films on SEM. Nonadherent surface properties such as the ion-bombarded silicone may be helpful in preventing chronic PE tube contamination.

Topics: Animals; Anti-Bacterial Agents; Biofilms; Catheters, Indwelling; Coated Materials, Biocompatible; Disease Models, Animal; Equipment Contamination; Fluorescent Antibody Technique; Guinea Pigs; Microscopy, Electron, Scanning; Middle Ear Ventilation; Otitis Media with Effusion; Oxides; Random Allocation; Recurrence; Silicones; Silver Compounds; Staphylococcus aureus; Surface Properties