diphenylhexatriene and Tuberculosis--Pulmonary

diphenylhexatriene has been researched along with Tuberculosis--Pulmonary* in 2 studies

Other Studies

2 other study(ies) available for diphenylhexatriene and Tuberculosis--Pulmonary

Article	Year
Natural and probe fluorescence of lymphocytes of patients with pulmonary tuberculosis exposed to radiation as a result of the Chernobyl nuclear accident. Archivum immunologiae et therapiae experimentalis, 1997, Volume: 45, Issue:5-6 Parameters of natural and probe fluorescence of peripheral blood lymphocytes have been studied in 25 normal persons and 72 patients suffering from extensive forms of pulmonary tuberculosis who live in various radioecological conditions. It was found that in cell suspension the intensity of fluorescence of protein and the membrane-bound merocyanine 540 dye was 2.0-3.2 and 3.9-7.1 times higher, respectively, for the lymphocytes of the examined patients. For lymphocytes of the patients who live in radionuclide-contaminated areas fluorescence of reduced pyridine nucleotides and some fluorescent probes (ANS, DPGT, the ratio of eximer to monomer fluorescence of pyrene) and the merocyanine-photosensitized cell death were also found to increase. These can indicate some changes of cell membranes and reduction of the oxidation resistance of lymphocytes. There was not any substantial dependence on the changes in the parameters determined in the patients calculated radiation doses was revealed. It was found that biophysical tests, in particular, photosensitized cell death, are informative for estimation of severity of the tuberculous process and prognosis of its outcome in patients who live in adverse radioecological conditions. Topics: Adult; Anilino Naphthalenesulfonates; Diphenylhexatriene; Fluorescence; Fluorescent Dyes; Humans; Lymphocytes; Male; Middle Aged; Photosensitizing Agents; Power Plants; Pyrimidinones; Radioactive Hazard Release; Republic of Belarus; Tuberculosis, Pulmonary; Ukraine	1997
[Measurement of fluidity of membrane lipids in lymphocytes from patients with pulmonary tuberculosis]. Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases, 1991, Volume: 14, Issue:2 The degree of fluidity of cellular membranes was determined by fluorescence polarization analysis using fluorescent probe 1,6-Dipheny-1,3,5-hexatriene (DPH), when embedded in membrane lipid region of intact cells. Results have shown that lymphocytes isolated from patients have a more fluid lipid layer in their cellular membrane (eta = 2.69 +/- 0.77 poise chi +/- s) than lymphocytes isolated from normal health (eta = 3.11 +/- 0.95 poise chi +/- s)(P less than 0.05). Increase in degree of membrane microviscosity was closely correlated with the rise in cells number. Topics: Cell Membrane; Diphenylhexatriene; Female; Fluorescence Polarization; Humans; Lymphocytes; Male; Membrane Fluidity; Membrane Lipids; Tuberculosis, Pulmonary	1991

Article

Year

Natural and probe fluorescence of lymphocytes of patients with pulmonary tuberculosis exposed to radiation as a result of the Chernobyl nuclear accident.

Archivum immunologiae et therapiae experimentalis, 1997, Volume: 45, Issue:5-6

Parameters of natural and probe fluorescence of peripheral blood lymphocytes have been studied in 25 normal persons and 72 patients suffering from extensive forms of pulmonary tuberculosis who live in various radioecological conditions. It was found that in cell suspension the intensity of fluorescence of protein and the membrane-bound merocyanine 540 dye was 2.0-3.2 and 3.9-7.1 times higher, respectively, for the lymphocytes of the examined patients. For lymphocytes of the patients who live in radionuclide-contaminated areas fluorescence of reduced pyridine nucleotides and some fluorescent probes (ANS, DPGT, the ratio of eximer to monomer fluorescence of pyrene) and the merocyanine-photosensitized cell death were also found to increase. These can indicate some changes of cell membranes and reduction of the oxidation resistance of lymphocytes. There was not any substantial dependence on the changes in the parameters determined in the patients calculated radiation doses was revealed. It was found that biophysical tests, in particular, photosensitized cell death, are informative for estimation of severity of the tuberculous process and prognosis of its outcome in patients who live in adverse radioecological conditions.

Topics: Adult; Anilino Naphthalenesulfonates; Diphenylhexatriene; Fluorescence; Fluorescent Dyes; Humans; Lymphocytes; Male; Middle Aged; Photosensitizing Agents; Power Plants; Pyrimidinones; Radioactive Hazard Release; Republic of Belarus; Tuberculosis, Pulmonary; Ukraine

1997

[Measurement of fluidity of membrane lipids in lymphocytes from patients with pulmonary tuberculosis].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases, 1991, Volume: 14, Issue:2

The degree of fluidity of cellular membranes was determined by fluorescence polarization analysis using fluorescent probe 1,6-Dipheny-1,3,5-hexatriene (DPH), when embedded in membrane lipid region of intact cells. Results have shown that lymphocytes isolated from patients have a more fluid lipid layer in their cellular membrane (eta = 2.69 +/- 0.77 poise chi +/- s) than lymphocytes isolated from normal health (eta = 3.11 +/- 0.95 poise chi +/- s)(P less than 0.05). Increase in degree of membrane microviscosity was closely correlated with the rise in cells number.

Topics: Cell Membrane; Diphenylhexatriene; Female; Fluorescence Polarization; Humans; Lymphocytes; Male; Membrane Fluidity; Membrane Lipids; Tuberculosis, Pulmonary

1991