dinoprost and Ovarian-Cysts

Resumption of ovulatory activity and the timely lysis of the first CL postpartum (pp) are important determinants for the reproductive performance of dairy cows. Cystic ovarian follicles (COFs) and persistent CLs preclude normal ovarian cyclicity and increase the calving interval. The objective of this study was to investigate the effect of GnRH on the incidence of COFs and the effect of PGF2α on the incidence of a prolonged luteal phase (PLP) and on fertility parameters in dairy cows. A total of 476 cows were examined ultrasonographically for the presence of a dominant follicle (12-25 mm, without CL >10 mm; n = 237) or a functional CL (≥20 mm; n = 239) between 28 and 35 days pp and were allocated to one of four groups. Cows with a dominant follicle received 10-μg GnRH (buserelin; group F-T; n = 118) or saline (group F-C; n = 119), and cows with a functional CL received 0.5 mg of a PGF2α analogue (cloprostenol; group CL-T; n = 119) or saline (group CL-C; n = 120) on the day of initial examination, defined as Day 0. Cows were reexamined 7 and 21 days (F-T and F-C) and 3 and 24 days (CL-T and CL-C) later, and COFs were treated immediately after diagnosis in all cows. On the basis of the ovarian findings on Days 21 and 24, cows were treated according to a protocol aimed at timely breeding. The incidence of COFs by Days 7 (F-T vs. F-C; 7.6% vs. 16.8%) and 21 (11.0% vs. 21.8%) decreased (P ≤ 0.03) with GnRH; however, this did not lead to a substantial improvement of calving-to-conception interval (means ± standard error of the mean; 107.91 ± 5.70 vs. 117.94 ± 6.63 days), first-service conception rate (42.3% vs. 41.3%), and number of services per conception (2.06 ± 0.12 vs. 2.31 ± 0.15). Treatment with PGF2α decreased (P < 0.0001) the incidence of PLP by Day 24 (CL-T vs. CL-C; 1.7% vs. 17.5%), decreased calving-to-conception interval(91.28 ± 4.77 vs. 101.75 ± 5.03 days), increased first-service conception rate (63.3% vs. 38.7%), and reduced the number of services per conception (1.65 ± 0.10 vs. 2.08 ± 0.12; each P ≤ 0.01). The results indicate that strategic treatment with GnRH or PGF2α in week 5 pp to induce early ovulation and luteolysis reduces the incidence of COFs and PLP, respectively. Initial treatment with PGF2α also enhanced reproductive performance when used in conjunction with a standardized treatment protocol for all cows in week 8 pp (aimed at timely breeding). In contrast, GnRH did not improve fertility parameters of cystic cows in herds where all

Topics: Animals; Cattle; Cloprostenol; Corpus Luteum; Dairying; Dinoprost; Female; Fertility; Gonadotropin-Releasing Hormone; Incidence; Luteolysis; Ovarian Cysts; Ovarian Follicle; Postpartum Period; Pregnancy; Reproduction

To study the ovarian response to the long-term effect of PGF2α, 16 cows were treated with 25 mg tromethamine dinoprost (Pronalgon F; Pfizer, Tokyo, Japan) for 21 days after natural ovulation. Five control cows were treated with sterile physiological saline. The follicle and corpus luteum (CL) development were monitored using a real-time ultrasound instrument. In addition, the plasma concentration of progesterone (P4) was determined. In nine of the 16 Pronalgon-treated cows, the first dominant follicle (1st DF), second dominant follicle (2nd DF), and third dominant follicle ovulated consecutively (group A). In five cows, the 1st and 2nd DFs ovulated consecutively (group B). The developing CL started to regress approximately 5 days after each ovulation without maturation in groups A and B. In the two remaining Pronalgon-treated cows, there was no further ovulation after natural ovulation (group C). In one cow in group C, the 1st DF became atretic and the 2nd DF became cystic with the diameter of the cystic follicle reaching 31.2 mm on Day 30. In another cow, the 1st DF became cystic with a diameter of 30.9 mm on Day 18. Although P4 began to increase after each ovulation in all of the Pronalgon-treated cows, it decreased immediately after each ovulation without a large increase, peaking at approximately 1 ng/mL. Furthermore, the number of days when P4 was >1 ng/mL from natural ovulation to Day 21 was 2.6 ± 0.7 days, which was significantly less than that in the control cows (16.0 ± 0.6 days). These results indicate that the long-term effect of PGF2α has an important role in ovulation of all dominant follicles and might induce cystic ovaries in cows.

Topics: Animals; Cattle; Cattle Diseases; Corpus Luteum; Dairying; Dinoprost; Female; Ovarian Cysts; Ovarian Follicle; Oxytocics; Progesterone; Ultrasonography

Despite the variety of hormonal treating regimes used, a proportion of cows with cystic ovarian disease (COD) fail to be cured. The hypothesis of this study was that cyst aspiration would improve the curing rate and/or accelerate the resumption of ovarian activity in affected cows. In four groups of cows the following treatments were administered: Group A (n = 18) only cyst aspiration, Group AGP (n = 19) cyst aspiration and a combination of GnRH and PGF 2alpha , Group GP (n = 25) only GnRH and PGF 2alpha , and Group C (n = 15) untreated control. Cysts were aspirated without ultrasonographic guidance, using a new device. All cows from Group AGP responded to treatment, while 5.5% from Group A and 16% from Group GP remained anoestrous (P < 0.05). These refractory cases were re-treated with the AGP protocol and exhibited oestrus within 12.4 +/- 1.1 days. The interval from PGF 2alpha injection to oestrus was significantly reduced in cows treated with cyst aspiration. Until day 80 post partum (pp), 11 of the 15 untreated cows (73.3%) retained the initial cyst and remained anoestrous. It is concluded that persisting cases of COD can be treated by combining aspiration with a hormonal regime. The technique presented here has no complication for the cow, is efficient, easy, safe for the operator, and requires low-cost equipment.

Topics: Animals; Buserelin; Cattle; Cattle Diseases; Dinoprost; Drainage; Female; Fertility Agents, Female; Gonadotropin-Releasing Hormone; Ovarian Cysts; Oxytocics

The benefit of using timed-insemination in lactating dairy cows for the treatment of ovarian cysts lies in the fact that cows do not have to be detected in estrus for insemination and achieving pregnancy. We compared the effectiveness of synchronization of ovulation with timed-insemination and induction of estrus with insemination at estrus in the treatment of bovine ovarian cysts in lactating dairy cows. After Day 65 post partum, a total of 368 lactating dairy cows was divided into 3 groups. Cows in Group 1 (n = 209, normal, noncystic) were treated with 100 ug, i.m. GnRH on Day 0; 25 mg, i.m. PGF2 alpha on Day 7; and 100 ug, i.m. GnRH on Day 9 and then were time-inseminated 16 h later. Cows in Group 2 (n = 76, abnormal, cystic) were treated with 100 ug, i.m. GnRH on Day 0; 25 mg, i.m. PGF2 alpha on Day 7; and 100 ug, i.m. GnRH on Day 9 and time-inseminated 16 h later. Cows in Group 3 (n = 83, abnormal, cystic) were treated with 100 ug, i.m. GnRH on Day 0; 25 mg, i.m. PGF2 alpha on Day 7; and inseminated at induced estrus within 7 d after treatment with PGF2 alpha. Day 0 was the day of initiation of the study. Conception and pregnancy rates among groups were compared using logistic regression and adjusted for parity, time of year and days in milk. Conception and pregnancy rates of Group 1 cows (31.5%) were not significantly different from those of Group 2 cows (23.6%). However, the pregnancy rate in normal cows (Group 1) was higher (P < 0.01) than in cystic cows (Groups 2 and 3). Cows in Group 3 had a higher conception rate than cows in Group 2 (51.7% > 23.6%; P < 0.01). However, pregnancy rates for cows in Groups 2 (23.6%) and 3 (18%) were not significantly different. The finding indicated that synchronization of ovulation and timed-insemination resulted in pregnancy rates similar to those of synchronization of estrus and insemination at an induced estrus within 7 d for the treatment of ovarian cysts in lactating dairy cows.

Topics: Animals; Cattle; Cattle Diseases; Dinoprost; Estrus; Estrus Detection; Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Lactation; Ovarian Cysts; Ovulation Induction; Pregnancy; Time Factors

The aim of this study was to establish a model to induce cystic ovarian follicles (COFs) in cattle using the cyclooxygenase inhibitor, indomethacin. Eighteen Holstein-Frisian cattle were synchronized with prostaglandin F2alpha (PGF2α) and gonadotropin-releasing hormone (GnRH). Ultrasound-guided transvaginal intrafollicular injections were performed in 23 preovulatory follicles with different concentrations of indomethacin 16 h after GnRH administration. An injection of 0.2 ml 35 µM indomethacin solution (resulting in a final concentration of 8 µg/ml in the follicular fluid) was the minimal dosage leading to COF formation. The induced COFs reached a maximum mean diameter of 36.9 ± 4.5 mm eleven days after injection. The estrous cycle was extended to 25-39 days. Luteinization was first observed 4 days after injection, accompanied by a slight increase in plasma progesterone concentration. The bioactivity of indomethacin was demonstrated by the decrease of prostaglandin E

Topics: Animals; Cattle; Cyclooxygenase Inhibitors; Dinoprost; Disease Models, Animal; Estrus Synchronization; Female; Gonadotropin-Releasing Hormone; Indomethacin; Ovarian Cysts; Ovarian Follicle; Ovulation

The treatment of cystic ovarian disease (COD) in dairy cows is still controversial, and some researchers recommend using gonadotropin-releasing hormone (GnRH) regardless of the type of cysts. The aim of this study was to comparatively evaluate the reproductive performance of cows diagnosed with follicular or luteal cystic structures, after treatment with either buserelin acetate (GnRH agonist) or dinoprost (prostaglandin F2-alpha or PGF

Topics: Animals; Buserelin; Cattle; Dinoprost; Estrus Synchronization; Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Ovarian Cysts; Pregnancy; Progesterone; Reproduction

To evaluate 8-hydroxy-2-deoxyguanosine (8-OHdG) and 8-isoprostane levels in the peritoneal fluid (PF) of women with endometriosis.. One hundred and ten women with laparoscopically and histopathologically confirmed endometriosis and, as reference groups, 119 patients with simple serous (n=78) and dermoid (n=41) ovarian cysts were studied. Peritoneal fluid 8-OHdG and 8-isoprostane concentrations were evaluated by enzyme-linked immunosorbent assays.. 8-OHdG and 8-isoprostane levels in peritoneal fluid were significantly higher in patients with endometriosis compared with the reference groups. Higher PF 8-OHdG and 8-isoprostane concentrations were observed in patients with advanced stages of endometriosis. A statistically significant positive correlation was found between 8-OHdG and 8-isoprostane levels in peritoneal fluid.. Endometriosis induces greater oxidative stress and frequent DNA mutations in peritoneal fluid than nonendometriotic ovarian cysts. The most severe oxidative stress occurs in the peritoneal cavity of women with more advanced stages of the disease.

Topics: 8-Hydroxy-2'-Deoxyguanosine; Adolescent; Adult; Ascitic Fluid; Biomarkers; Cysts; Deoxyguanosine; Dermoid Cyst; Dinoprost; Endometriosis; Female; Humans; Middle Aged; Mutation; Ovarian Cysts; Ovarian Neoplasms; Oxidative Stress; Peritoneum; Severity of Illness Index; Up-Regulation; Young Adult

Topics: Animals; Cattle; Cattle Diseases; Dinoprost; Fatty Acids, Nonesterified; Female; Hydrocortisone; Insulin-Like Growth Factor Binding Proteins; Insulin-Like Growth Factor I; Ovarian Cysts; Parity; Pregnancy; Puerperal Disorders

The objective of this study was to investigate cystic ovarian disease (COD) in commercial Japanese Black cows and to evaluate the efficacy of 7-day insertion of an intravaginal progesterone insert (CIDR) combined with prostaglandin F(2alpha) (PGF(2alpha)) at CIDR removal. Experiment 1 was conducted to group cysts into 4 patterns based on alteration of plasma progesterone (P(4)) concentrations on d -7 and d 0 (=CIDR insertion) with 1.0 ng/ml as the cut-off level by ultrasonographic examination of 28 cows with COD that were >or=40 days postpartum and anoestrous after calving. In Experiment 2, a total of 55 cows under the same conditions as in Experiment 1 were utilized, and the same regimen as in Experiment 1 was performed without 7 days of pre-observation before treatment. As a result, 92.9% of CLs on d 21 were highly formed in Experiment 1 and 83.6% were highly formed in Experiment 2. The conception rates within 60 days after CIDR removal were also satisfactory high and were 71.4% and 54.5%, respectively. There were no differences in any overall reproductive parameters between Experiments 1 and 2 (P>0.05). The average days between CIDR removal and conception were 24.4 +/- 5.3 and 24.0 +/- 6.5 days, respectively (P>0.05); therefore, the conception dates of the cows in Experiment 2 were at least 7 days earlier compared with Experiment 1. In conclusion, treatment with a CIDR and PGF(2alpha) against COD could minimize the risk of incorrect treatment and provide sufficient reproductive performance in Japanese Black cows.

Topics: Administration, Intravaginal; Animals; Cattle; Cattle Diseases; Dinoprost; Female; Male; Ovarian Cysts; Postpartum Period; Pregnancy; Progesterone; Reproduction; Ultrasonography

The objective of this study was to compare the effectiveness of the Ovsynch and controlled internal drug releasing (CIDR) protocols under commercial conditions for the treatment of cystic ovarian disease in dairy cattle. A total of 401 lactating dairy cows with ovarian cysts were alternatively allocated to two treatment groups on the day of diagnosis. Cows in the Ovsynch group were treated with GnRH on Day 0, PGF2alpha on Day 7, GnRH on Day 9, with timed insemination 16-20 h later. Cows in the CIDR group were treated with a CIDR insert on Day 0 for 7 days; on Day 7, the CIDR was removed, and cows were treated with PGF2alpha. All cows in the CIDR group were observed for estrus and cows exhibiting estrus within 7 days following removal of the CIDR and PGF2alpha administration were inseminated. The outcomes of interest for this experiment were the likelihood to be inseminated, return to cyclicity (determined by a CL on Day 21), conception and pregnancy rates. Data for these variables were analyzed using logistic regression. The percentage of cows inseminated in the Ovsynch and CIDR groups were 82 and 44%, respectively. Cows in the Ovsynch group were 5.8 times more likely to be inseminated than cows in the CIDR group. Cows with a low BCS were 0.48 times less likely to be inseminated than cows with a high BCS. The percentage of cows with a CL on Day 21 for the Ovsynch and CIDR groups was 83 and 79%, respectively (P > 0.05). Cows with a low BCS were 0.49 times less likely to have CL on Day 21 than cows with a high BCS. Conception and pregnancy rates for cows in the Ovsynch group were 18.3 and 14.4%, respectively. Conception and pregnancy rates for cows in the CIDR group were 23.1 and 9.5%, respectively. There was no significant differences between conception or pregnancy rates in cows in both groups. Primiparous cows were 2.6 times more likely to conceive than multiparous cows. In conclusion, the results of this study suggested that fertility was not different between cows with ovarian cysts treated with either the Ovsynch or the CIDR protocols in this dairy herd. In addition, primiparous cows had an increased likelihood for conception compared to multiparous cows, and cows with a low BCS were less likely to be inseminated or have a CL on Day 21, regardless of treatment.

Topics: Animals; Body Constitution; Cattle; Cattle Diseases; Dinoprost; Drug Implants; Estrus; Estrus Detection; Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Lactation; Logistic Models; Ovarian Cysts; Ovulation Induction; Parity; Pregnancy; Pregnancy Rate; Progesterone; Random Allocation; Time Factors

The objective of this study was to evaluate the association between the level of milk production on the day of diagnosis of ovarian cysts and treatment response using the Ovsynch protocol. On the day of cyst diagnosis (Day 0), 260 lactating dairy cows with ovarian cysts were treated with gonadotropin-releasing hormone (GnRH), PGF2alpha on Day 7, GnRH on Day 9, and timed inseminated 16-20 h later (Ovsynch protocol). Pregnancy was determined (by transrectal palpation) between 42 and 49 days after insemination. On Day 0, data for milk production (kg/day), parity, days in milk (DIM), and body condition score (BCS) were recorded. Using the median value for milk production on the day of diagnosis, cows were classified as high producers (>28.5 kg) and low producers (or=0.05). Primiparous cows were more likely (adjusted odds ratio: AOR=3.63; 95% CI: 95% confidence intervals=1.28-10.30; P

Topics: Animals; Body Constitution; Cattle; Cattle Diseases; Dinoprost; Drug Implants; Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Lactation; Logistic Models; Milk; Ovarian Cysts; Ovulation Induction; Parity; Pregnancy; Pregnancy Rate; Time Factors; Treatment Outcome

The objective of this study was to compare the economic benefits of timed artificial insemination (AI) and a progesterone insert as therapeutic treatments for cows diagnosed with cystic ovarian disease (COD). A secondary objective was to illustrate the use of a stochastic dynamic simulation model to fully account for all changes in revenues and costs affected by differences in treatments. First, 4 herds of 1,000 cows each were simulated until steady state. These cows were free from COD and inseminated based on estrus only. Herds differed by probability of estrus detection (46 or 70%) and days in milk (DIM) when nonpregnant cows were culled (330 or 400 d). Second, 3 herds were created with 1,000 nonpregnant cows at 90, 170, or 250 DIM. These cows were considered diagnosed with COD at the start of the simulation (d 0); no new cases of COD developed after d 0. Cows spontaneously recovered or were treated. Treatments were either timed AI or intravaginal device containing progesterone followed by PGF(2alpha) and then AI if estrus was detected. Effects of treatments were evaluated in 48 scenarios based on compliance of timed AI (82 or 100%), probability of estrus detection (46 or 70%), and DIM when nonpregnant cows were culled (330 or 400 d). As cows became pregnant or were replaced, the herd evolved into the associated steady-state herd. Seven scenarios resulted in less than 50% of cows conceiving before they were culled. The percentage of cows diagnosed with COD that calved again ranged from 14.0 to 74.4% and was significantly reduced when COD was diagnosed later in lactation. Treatments in all cases were more valuable than waiting for spontaneous recovery. The average values of timed AI (82 or 100% compliance) and the progesterone insert were 83.29 dollars, 86.83 dollars, and 71.89 dollars, respectively, compared with waiting for spontaneous recovery. Treatments were least beneficial at 90 DIM. The benefits of timed AI (82 or 100% compliance) compared with the progesterone insert, adjusted for DIM and days to culling, were 14.98 dollars and 21.53 dollars when the probability of estrus detection was 46%. At 70% probability of estrus detection, the benefits were 7.81 dollars and 8.34 dollars, respectively. Overall benefit of treatment by timed AI was 11.39 dollars greater than by progesterone insert.

Topics: Administration, Intravaginal; Animals; Cattle; Cattle Diseases; Cost-Benefit Analysis; Dairying; Dinoprost; Eating; Estrus Detection; Fats; Female; Insemination, Artificial; Lactation; Milk; Ovarian Cysts; Pregnancy; Progesterone; Time Factors

Pregnancy rates were compared in lactating dairy cows (n = 1083) assigned to protocols for resynchronization of ovulation based on stages of the estrous cycle, or presence of ovarian cysts or anestrus. Cows were detected not pregnant by ultrasonography 30 d after a previous AI (study day 0) and classified as diestrus, metestrus, proestrus, with ovarian cysts or anestrus. Cows in diestrus (January-May) were assigned to either Ovsynch (GnRH day 0, PGF2alpha day 7, GnRH day 9, and timed-AI [TAI] 16 h later; n = 96), or Quicksynch (PGF2alpha day 0, estradiol cypionate [ECP] day 1, AI at detected estrus [AIDE] on day 2, or TAI on day 3; n = 96). Cows in diestrus (June-December) were assigned to either Ovsynch (n = 156) or Modified Quicksynch (PGF2alpha day 0, ECP day 1, AIDE days 2 and 3, and to Ovsynch on day 4 if not detected in estrus; n = 142). Cows in metestrus were assigned either to Ovsynch (n = 68), Heatsynch (GnRH day 0, PGF2alpha day 7, ECP day 8, AIDE day 9, or TAI day 10; n = 62), or GnRH + Ovsynch (GnRH on day 0, followed by Ovsynch on day 8; n = 64). Cows in proestrus, with ovarian cysts, or anestrus were assigned to either Ovsynch (proestrus n = 89, ovarian cysts n = 97, anestrus n = 8) or GnRH + Ovsynch (proestrus n = 87, ovarian cysts n = 109, anestrus n = 9). Pregnancy rate was evaluated 30, 55 and 90 d after resynchronized AI. For cows in diestrus (January-May), pregnancy rates were higher for Ovsynch (35.9, 29.2 and 26.0%) than for Quicksynch (21.7, 16.7 and 15.6%). For cows in diestrus (June-December), pregnancy rates were similar for Ovsynch (34.4, 24.0 and 23.6%) and Modified Quicksynch (27.1, 26.2 and 21.6%). For cows in metestrus, pregnancy rates were higher for GnRH + Ovsynch (33.3, 24.5 and 20.3%) than for Heatsynch (20.3, 12.9 and 9.8%). For cows with ovarian cysts, pregnancy rates were higher for GnRH + Ovsynch (30.3, 26.6 and 22.9%) than for Ovsynch (20.2, 18.5 and 14.7%). Assignment to resynchronization protocols based on the stages of the estrous cycle, or presence of ovarian cysts improved pregnancy rates.

Topics: Anestrus; Animals; Cattle; Cattle Diseases; Diestrus; Dinoprost; Estradiol; Estrous Cycle; Estrus Synchronization; Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Lactation; Metestrus; Ovarian Cysts; Ovulation Induction; Pregnancy; Proestrus; Seasons; Time Factors; Ultrasonography

The objective was to compare pregnancy rates to resynchronization and timed AI (TAI) protocols in lactating dairy cows that received GnRH at 23 d and were diagnosed not pregnant at 30 d after the pre-enrollment AI. Nonpregnant cows (624) at ultrasonography on day 30 (study day 0) were classified as diestrus (74.8%), metestrus (5.6%) and without a CL (19.5%). Cows in diestrus were assigned either to the GnRH group (PGF2alpha on day 0, GnRH on day 2 and TAI 16 h later, n = 238) or the estradiol cypionate (ECP) group (PGF2alpha on day 0, ECP on day 1, and TAI 36 h later, n = 229). Cows in metestrus were assigned to the Modified Heatsynch Group (GnRH on day 0, PGF(2alpha) on day 7, ECP on day 8 and TAI on day 9, n = 35). Cows without a CL (n = 122) were classified either as proestrus (10.6%), ovarian cysts (7.5%) or anestrus (1.4%), and assigned to factorial treatments (i.e., use of GnRH versus CIDR) to either the GnRH group (GnRH on day 0, PGF2alpha on day 7, GnRH on day 9 and TAI 16 h later, n = 28), the CIDR group (CIDR insert from days 0 to 7, PGF2alpha on day 7, GnRH on day 9 and TAI 16 h later, n = 34), the GnRH + CIDR group (GnRH on day 0, CIDR insert from days 0 to 7, PGF2alpha on day 7, GnRH on day 9 and TAI 16h later, n = 32), and the control group (PGF2alpha on day 7, GnRH on day 9 and TAI 16 h later, n = 28). For cows without a CL, plasma P4 concentrations were determined on days 0, 7, 10 and 17 and ovarian structures determined on days 0, 7 and 17. Pregnancy rates were evaluated at 30, 55 and 90 d after the resynchronized AI. For cows in diestrus, there were no differences in pregnancy rates on days 30, 55 and 90 for cows in the GnRH (27.5, 26.5 and 24.2%) or ECP (29.1, 25.5 and 24.1%) groups. In addition, there were no differences in pregnancy losses between days 30 and 55 and 55 and 90 between the GnRH (7.0 and 8.6%) and ECP (9.8 and 5.4%) groups. For cows without a CL, GnRH on day 0 increased the proportion of cows with a CL on days 7 and 17 and plasma P4 concentration on day 17 in cows with ovarian cysts but not for cows in proestrus. The CIDR insert increased pregnancy rate in cows with ovarian cysts but reduced pregnancy rate for cows in proestrus.

Topics: Animals; Cattle; Cattle Diseases; Diestrus; Dinoprost; Estradiol; Estrus Synchronization; Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Lactation; Metestrus; Ovarian Cysts; Ovulation Induction; Pregnancy; Proestrus; Progesterone; Time Factors; Ultrasonography

Cystic ovarian disease is an important cause of reproductive failure. The objective of this study was to evaluate transrectal ultrasonography as a diagnostic tool and gonadotropin-releasing hormone (GnRH) as a therapeutic approach for ovarian follicular cysts in goats. Goats were considered to have a follicular cyst(s) if a non-echoic structure >10 mm in diameter was detected in the absence of corpora lutea (CL) in three ultrasonic examinations performed at 5-day intervals. After diagnosis (Day 0), goats with ovarian follicular cysts (n = 5) were treated with a single bolus injection of 10.5 microg synthetic GnRH followed by administration of 125 microg prostaglandin F2alpha (PGF2alpha) 10 days later. Five blood samples were collected at 5-day intervals for determination of progesterone and estradiol-17beta. For detection of LH surge, blood samples were collected every 2 h. Ovulation rate was determined and pregnancy was confirmed by transrectal ultrasonography. The results showed that transrectal ultrasonography is reliable for diagnosis of ovarian follicular cysts and the mean diameter of the follicular cysts was 12.6 +/- 0.4 mm. Plasma concentrations of progesterone and estradiol-17beta at the time of diagnosis of follicular cysts (Day 0) were 0.7 +/- 0.2 ng/ml and 12.7 +/- 0.9 pg/ml, respectively. The concentration of progesterone increased to 4.0 +/- 0.5 ng/ml 10 days after administration of GnRH indicating luteinization of the ovarian follicular cysts concomitant with a decrease in the concentration of estradiol-17beta (3.5 +/- 0.4 pg/ml). Administration of GnRH to cystic goats resulted in a surge of LH within 2 h of treatment. The interval from PGF2alpha injection to the preovulatory LH surge was 62.8 +/- 1.4 h. All goats exhibited estrus 55.2 +/- 2.3 h after PGF2alpha injection and four goats out of the five ovulated. The ovulation rate was 1.5 +/- 0.3. In conclusion, results of this study suggest that transrectal ultrasonography is a reliable tool for diagnosis of ovarian follicular cysts. In addition, GnRH can be used to effectively treat ovarian follicular cysts in goats with 80% success rate.

Topics: Animals; Dinoprost; Estradiol; Estrus; Female; Goat Diseases; Goats; Gonadotropin-Releasing Hormone; Infertility, Female; Luteinizing Hormone; Ovarian Cysts; Ovarian Follicle; Ovulation; Pregnancy; Progesterone; Rectum; Time Factors; Ultrasonography

We compared outcomes of 2 protocols used to resynchronize estrus and ovulation in dairy females after a not-pregnant diagnosis. Nulliparous heifers and lactating cows in which artificial insemination (AI) occurred 41 +/- 1 d earlier were presented every 2 to 3 wk for pregnancy diagnosis by using ultrasonography. Ovaries were scanned, follicles were mapped and sized, presence of corpus luteum was noted, and GnRH was injected (d 0). Females were assigned randomly to receive PGF(2alpha) 7 d later (d 7) and then either received estradiol cypionate (ECP) 24 h after PGF(2alpha) (d 8; Heatsynch; n = 230) or a second GnRH injection 48 h after PGF(2alpha) (d 9; Ovsynch; n = 224). Those detected in estrus since their not-pregnant diagnosis were inseminated, whereas the remainder received a timed AI (TAI) between 65 and 74 h after PGF(2alpha). Ovarian scans and blood collected before injections for progesterone analysis were used to classify 4 ovarian status groups: anestrus, follicular cysts, luteal cysts, and cycling, plus an unknown group of females in which no blood sampling or ovarian scans were made. Few females (5.1%) were inseminated between not-pregnant diagnosis and d 8. On d 10, more ECP- than GnRH-treated females were inseminated after detected estrus (24 vs. 6%). Overall, more Ovsynch than Heatsynch females received a TAI (82 vs. 62%). Conception rates tended to be greater for females inseminated after estrus (37%) than after TAI (29%), particularly for those treated with Heatsynch (41 vs. 27%) than with Ovsynch (33 vs. 31%). Those inseminated after estrus conceived 31 +/- 8 d sooner than those receiving the TAI. Conception rates for females having elevated progesterone 7 d after the not-pregnant diagnosis were greater than those having low progesterone in Heatsynch (42%; n = 133 vs. 25%; n = 55) and Ovsynch protocols (33%; n = 142 vs. 15%; n = 45), respectively. Conception rates were greater in nulliparous heifers than in lactating cows (43 vs. 28%) regardless of protocol used. Although overall pregnancy outcomes after a not-pregnant diagnosis were similar in response to either the Ovsynch and Heatsynch protocols, inseminations performed after detected estrus before the scheduled TAI reduced days to eventual conception and tended to increase conception rates, particularly after Heatsynch.

Topics: Animals; Cattle; Cattle Diseases; Dinoprost; Estradiol; Estrus; Estrus Detection; Estrus Synchronization; Female; Fertility Agents, Female; Gonadotropin-Releasing Hormone; Insemination, Artificial; Ovarian Cysts; Parity; Pregnancy; Pregnancy Rate; Pregnancy, Animal; Progesterone; Random Allocation; Treatment Outcome; Ultrasonography

To identify oxidative stress in peritoneum during laparoscopic and open surgery by measuring products of lipid peroxidation, and to determine whether surgical approach influences the type of oxidative metabolite synthesized.. Retrospective analysis (Canadian Task Force classification II-2).. University-affiliated hospital.. Twenty-eight consecutive women with uterine myomas or ovarian cysts.. Laparoscopic or open surgery (14 patients each).. We obtained 1 x 1-cm squares of peritoneum at the beginning and end of surgical procedures away from sites of surgery. 8-Isoprostaglandin F(2alpha), hydroxyeicosatetranoic acids (HETEs), and malondyaldehyde (MDA) were measured by enzyme-immunoassay, high-performance liquid chromatography, and thiobarbituric acid adduction method, respectively. Comparisons showed significant increases in 5-HETE and 8-prostane in the laparoscopy group, which were correlated with duration of pneumoperitoneum and volume of carbon dioxide (CO(2)) insufflated, respectively. In the laparotomy group only MDA rose significantly related to duration of surgery.. Lipid peroxidation was observed in peripheral peritoneum during laparoscopic surgery, mediated through noncyclooxygenase and lipoxygenase pathways, and appears to be due to effects of CO(2) pneumoperitoneum. Biochemical reactions were also observed in the laparotomy group, but are thought to be related to mechanisms other than lipid peroxidation.

Topics: Adult; Chromatography, High Pressure Liquid; Cohort Studies; Dinoprost; Enzyme-Linked Immunosorbent Assay; Female; Humans; Hydroxyeicosatetraenoic Acids; Laparoscopy; Laparotomy; Leiomyoma; Lipid Peroxidation; Malondialdehyde; Middle Aged; Ovarian Cysts; Oxidative Stress; Peritoneum; Pneumoperitoneum, Artificial; Probability; Retrospective Studies; Sensitivity and Specificity; Statistics, Nonparametric; Uterine Neoplasms

Two experiments in lactating dairy cows examined ovarian follicular responses to high, frequent doses of exogenous LH pulses at levels associated with follicular cysts. In Experiment 1, estrus was synchronized in 12 cyclic lactating cows >40 d postpartum. Emergence of the second follicular wave (d 0) was determined by ultrasonography. Starting on d 1, cows received LH (40 microg/h; n = 7) or saline (2 mL/h; n = 5) in hourly pulses for up to 5 (n = 5) or 7 (n = 7) d. On d 2, all cows received two injections of PGF2alpha, 12 h apart. In experiment 2, 14 lactating cows (7 to 12 d postpartum) received LH (40 microg/h; n = 7) or saline (1 mL/h; n = 7) in hourly pulses for 7 d, beginning 24 h after start of the first follicular wave. Daily samples were used to determine serum concentrations of progesterone (P4), estradiol-17beta (E2), LH, and FSH. Profiles of LH were determined from blood samples collected at 12-min intervals for 8 h on d 3. During infusion of LH, serum P4 and FSH were similar across treatments in both experiments. Serum E2 concentrations were similar in experiment 1, but serum E2 was greater on d 2, 3, and 5 in LH-treated cows in experiment 2. Infusion increased LH pulse frequency and amplitude in both experiments. Formation of cysts did not differ between LH- and saline-treated cows in either experiment (1 of 7 vs. 0 of 5 and 1 of 6 vs. 0 of 7, respectively). Cows that ovulated had similar intervals to ovulation in experiment 1 [6.0 +/- 0.1 d (LH) vs. 6.4 +/- 0.2 d (saline)], but in experiment 2, ovulation was 14 d earlier in LH-treated cows (5.6 +/- 1.8 d vs 19.9 +/- 1.5 d). In conclusion, high concentrations of LH are not solely responsible for formation of cysts in lactating dairy cows. Pulsatile infusion of LH stimulated follicular growth and steroidogenesis and decreased time to first ovulation in anestrous postpartum cows.

Topics: Animals; Cattle; Cattle Diseases; Dinoprost; Estradiol; Estrous Cycle; Estrus Synchronization; Female; Follicle Stimulating Hormone; Lactation; Luteinizing Hormone; Ovarian Cysts; Ovarian Follicle; Ovulation; Periodicity; Postpartum Period; Progesterone

We used immunoneutralization of endogenous estradiol to investigate deficiencies in the estradiol-feedback regulation of LH secretion as a primary cause of follicular cysts in cattle. Twenty-one cows in the prostaglandin (PG) F(2alpha)-induced follicular phase were assigned to receive either 100 ml of estradiol antiserum produced in a castrated male goat (n = 11, immunized group) or the same amount of castrated male goat serum (n = 10, control group). The time of injection of the sera was designated as 0 h and Day 0. Five cows in each group were assigned to subgroups in which we determined the effects of estradiol immunization on LH secretion and follicular growth during the periovulatory period. The remaining six estradiol-immunized cows were subjected to long-term analyses of follicular growth and hormonal profiles, including evaluation of pulsatile secretion of LH. The remaining five control cows were used to determine pulsatile secretion of LH on Day 0 (follicular phase) and Day 14 (midluteal phase). The control cows exhibited a preovulatory LH surge within 48 h after injection of the control serum, followed by ovulation of the dominant follicle that had developed during the PGF(2alpha)-induced follicular phase. In contrast, the LH surge was not detected after treatment with estradiol antiserum. None of the 11 estradiol-immunized cows had ovulation of the dominant follicle, which had emerged before estradiol immunization and enlarged to more than 20 mm in diameter by Day 10. Long-term observation of the six immunized cows revealed that five had multiple follicular waves, with maximum follicular sizes of 20-45 mm at 10- to 30-day intervals for more than 50 days. The sixth cow experienced twin ovulations of the initial persistent follicles on Day 18. The LH pulse frequency in the five immunized cows that showed the long-term turnover of cystic follicles ranged from 0.81 +/- 0.13 to 0.97 +/- 0.09 pulses/h during the experiment, significantly (P < 0.05) higher than that in the midluteal phase of the control cows (0.23 +/- 0.07). The mean LH concentration in the immunized cows was also generally higher than that in the luteal phase of the control cows. However, the LH pulse and mean concentration of LH after immunization were similar to those in the follicular phase of the control cows. Plasma concentrations of total inhibin increased (P < 0.01) concomitant with the emergence of cystic follicles and remained high during the growth of cystic follicles, wher

Topics: Animals; Cattle; Cattle Diseases; Dinoprost; Estradiol; Feedback, Physiological; Female; Follicle Stimulating Hormone; Follicular Phase; Goats; Immune Sera; Immunization; Kinetics; Luteal Phase; Luteinizing Hormone; Male; Orchiectomy; Ovarian Cysts; Ovarian Follicle; Ovulation; Periodicity

To determine the effect of parity and milk production on the incidence of double ovulation, the synchronization of ovulation, using GnRH and prostaglandin F2 alpha followed by timed AI (Ovsynch), was initiated at a random stage of the estrous cycle in lactating Holstein cows (n = 237). Ovulatory response at 48 h after the second GnRH injection and conception rate at 28 d post AI were determined by transrectal ultrasonography. Ovulation was synchronized in 84% of cows receiving the Ovsynch protocol. Of the synchronized cows, 14.1% exhibited a double ovulation and 47.6% conceived. Conception rate tended to be greater (P = 0.08) for cows exhibiting double (64.0%) rather than single ovulation (45.2%). To determine the effect of milk production on the incidence of double ovulation, cows were classified into low (< or = 40 kg/d) or high (> 40 kg/d) milk production groups based on the average milk production of 40.5 +/- 0.8 kg/d collected 2 d before AI. Although the incidence of double ovulation tended to increase linearly (P = 0.09) with increasing parity, the incidence of double ovulation was nearly 3-fold greater (P < 0.05) for cows in the high (20.2%) than the low (6.9%) milk production group. Furthermore, the increase in the incidence of double ovulation with parity apparently occurred because, within a parity group, the proportion of cows with high milk production was greater for the older cows. Twinning rate of cows that calved (n = 58) was 5.2%. In a secondary objective, cows were retrospectively classified as cystic or normal based on ultrasonographic ovarian morphology at the time of the second GnRH injection. Incidence of ovarian cysts was 11%, and the synchronization and conception rate of cows classified as cystic was 73.1 and 36.8%, respectively, which did not differ from that of normal cows. We conclude that milk production is the primary factor affecting the incidence of double ovulation in lactating dairy cows and may explain the effect of parity on twinning rate. In addition, Ovsynch appears to be an effective method for establishing pregnancy in lactating dairy cows with ovarian cysts.

Topics: Animals; Cattle; Cattle Diseases; Dinoprost; Drug Administration Schedule; Female; Gonadotropin-Releasing Hormone; Incidence; Lactation; Milk; Ovarian Cysts; Ovulation; Ovulation Induction; Parity; Pregnancy; Twins

Data were collected on reproductive, health and production events in 45 dairy herds in southwestern and eastern Ontario, Canada, from July 1990 to July 1993. Mean and median days in milk at first estrus, at first service, and at conception were 85.1 and 83.9, 94.0 and 88.9, and 126.3 and 115.5 d, respectively. The mean estrus detection rate was 48.3%, with a minimum of 28.4% and a maximum of 64.2%. The mean overall conception rate was 46.7%, with a minimum of 29.8% and a maximum of 70.7%. The most commonly used used therapeutic agents were GnRH (11.5% lactational incidence risk) and PG (13.1% lactational incidence risk). Estrus management accounted for the largest portion of PG use in the herds studied (69.4%). Results of a two sample t-test indicated no significant difference in reproductive performance among herds housed in free-stall compared with those housed in tie-stall barns. Multivariate linear regression models of 3 reproductive performance measures indicated that cows with ovarian cysts and/or increased peak milk production had significantly longer calving-to-first estrus and calving-to-conception intervals as well as lower conception rates than average producing cows without cysts. Cows with metritis were found to have significantly shorter calving-to-conception intervals but no detectable difference in conception rate or calving-to-first estrus interval than their herdmates. As lactation number increased reproductive performance improved. Cows that were observed in estrus earlier conceived earlier. Animals receiving uterine infusions had significantly longer calving-to-conception intervals and lower conception rates than their herdmates. There was no detectable difference in reproductive performance for cows treated with GnRH or PG compared with the nontreated cows. An increased estrus detection rate was associated with shorter calving-to-conception intervals and lower conception rates. Based on the range of performance in the study herds, the factors with the greatest potential influence on calving-to-conception interval in the average herd were the estrus detection rate and conception rate.

Topics: Animals; Cattle; Cattle Diseases; Dairying; Dinoprost; Endometritis; Estrus; Female; Gonadotropin-Releasing Hormone; Lactation; Linear Models; Ontario; Ovarian Cysts; Pregnancy; Reproduction; Time Factors

This study assessed the value of ultrasonography in characterising bovine cystic ovaries and monitoring their responses to different treatments. Thirteen cows were diagnosed by ultrasonography as having luteinised ovarian cysts and seven were diagnosed as having follicular ovarian cysts. Six of the former were treated with prostaglandin, four with a progesterone intravaginal device (PRID) and three with gonadotrophin-releasing hormone (Gn-RH); five of the latter were treated with Gn-RH and two with a PRID. All the animals were re-examined by ultrasound and blood was collected for the measurement of plasma progesterone concentration at intervals until oestrus. The treatment of the luteinised cysts with prostaglandin caused marked decreases in size and plasma progesterone concentration and altered their echotexture within two to four days; oestrus occurred within three to four days. In two of the cows treated with a PRID the cysts regressed within one to two weeks but the other two cows required supplementary treatment with prostaglandin; oestrus and ovulation were observed only after the cysts collapsed. Gn-RH stimulated oestrus and ovulation within three to four days but the cysts did not collapse until much later. The treatment of the follicular cysts with Gn-RH or a PRID caused fresh ovulation and the formation of a corpus luteum but had little immediate effect upon the cyst. The plasma progesterone concentrations in some of the cows with either follicular or luteal cysts were similar on the day of treatment and were therefore of little value in differentiating the types of cyst.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Administration, Intravaginal; Animals; Cattle; Cattle Diseases; Cloprostenol; Corpus Luteum; Dinoprost; Estrus; Female; Gonadotropin-Releasing Hormone; Injections, Intramuscular; Ovarian Cysts; Ovulation; Pregnancy; Progesterone; Prostaglandins F; Radioimmunoassay; Ultrasonography

Laparoscopic injection has become an important tool in tubal pregnancy. Intratubal injection of 5 mg of prostaglandin F2 alpha (PG F2 alpha) was successful at a serum human chorionic gonadotropin (hCG) level of more than 2500 mlU/ml. Intrauterine pregnancy occurred 112 days after laparoscopic treatment, during earlier removal of the contralateral tube.

Topics: Adult; Chorionic Gonadotropin; Combined Modality Therapy; Dilatation and Curettage; Dinoprost; Fallopian Tubes; Female; Humans; Infant, Newborn; Injections, Intralesional; Laparoscopy; Male; Ovarian Cysts; Ovariectomy; Postoperative Complications; Pregnancy; Pregnancy, Tubal

Questionnaires regarding the use of prostaglandin F2 alpha and its analogues (hereafter referred to as PG) were sent to 332 Alabama beef cattle owners and to 279 Alabama dairy cattle owners after attempting to contact them by telephone to request their participation in the survey. Questionnaires concerning the use of PG in their clients' herds were likewise sent to 147 food animal and mixed animal practitioners in Alabama after attempting telephone contact. Response among beef cattle owners, dairy cattle owners, and veterinarians to whom questionnaires were mailed was 64.5, 61.6, and 75.5%, respectively. Only 7.4% (13 of 175) of respondent beef cattle owners reported use of PG in their herds, and this use was predominantly for artificial insemination and embryo transfer. In contrast, 66.5% (109 of 164) of respondent dairy cattle owners reported use of PG, generally with satisfactory results, for some of the following conditions: unobserved estrus (n = 77), uterine infections (n = 74), retained placenta (n = 65), cystic ovaries (n = 56), estrus synchronization (n = 45), and induction of parturition (n = 13). Although 94.9% of respondent veterinarians treated cattle with PG, those attending beef herds thought that more important strategies were available for improvement of beef cattle productivity than increased use of PG. Among these strategies were shorter calving seasons, improved nutrition, better record keeping, more frequent herd health visits, improved animal identification, and increased use of bull breeding soundness examinations.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Agriculture; Alabama; Animals; Breeding; Cattle; Dairying; Dinoprost; Drug Utilization; Embryo Transfer; Estrus Synchronization; Female; Insemination, Artificial; Labor, Induced; Male; Ovarian Cysts; Placenta Diseases; Pregnancy; Surveys and Questionnaires; Uterine Diseases; Veterinary Medicine

Manipulation of an ovary during the follicular phase in cycling gilts or prepubertal gilts treated with PMSG and hCG results in formation of cysts on manipulated ovaries and corpora lutea (CL) of normal appearance on nonmanipulated ovaries. In contrast, cysts did not form after manipulation in luteal phase gilts. In the present experiment, daily administration of 50 mg progesterone to prepubertal gilts treated with PMSG and hCG established luteal phase concentrations of progesterone but did not lessen the incidence of manipulated-induced cysts. Number of cysts formed was associated with the number of follicles > or = 5 mm at manipulation, which was inversely related to serum concentrations of progesterone. Number of receptors for LH/hCG in follicular tissues did not differ between manipulated and nonmanipulated ovaries but was greater in granulosa (P < .05) and theca (P < .08) from follicles with diameters > or = 7 mm compared to 5 and 6 mm. Contents of estradiol, androstenedione, testosterone, progesterone and prostaglandins E2 and F2 alpha in follicular fluid, granulosa and theca were not different between follicles > or = 5 mm destined to form cysts. Profiles of progesterone and estradiol in peripheral serum and duration of luteal phase concentrations of progesterone were not different for gilts with induced cysts and gilts with CL. In conclusion, manipulation of follicles resulted in a failure to ovulate. Subsequent formation of cysts did not result from or result in a loss of steroidogenic function or the ability to bind LH to follicular receptors. These results demonstrate that the mechanism for ovulation is independent of other follicular processes, since ovulation can be disrupted without altering follicular steriodogenesis or subsequent luteinization.

Topics: Animals; Chorionic Gonadotropin; Dinoprost; Dinoprostone; Disease Models, Animal; Female; Follicular Fluid; Gonadal Steroid Hormones; Gonadotropins, Equine; Granulosa Cells; Luteal Phase; Ovarian Cysts; Ovarian Follicle; Progesterone; Random Allocation; Receptors, LH; Swine; Swine Diseases; Theca Cells

Authors removed dextro-ovary of a patient having 7-9th week of gestation for ovarian cyst during abortion. Preoperative cervical dilation was performed by Enzaprost-F cervical tablet containing 20 mg PGF2 agent. Before and 2, 4 and 6 hours after treatment, serum-progesterone and 17-beta-estradiol level was determined. Drug cervical dilation enabled instrumental termination of pregnancy within 4 hours. Histological finding of yellow body showed initial signs of colloid-cystic degeneration, which was followed by minimal decrease of values of serum-steroid concentrations. Authors presume that change in histological picture of yellow body was caused by effect of cervical tablet containing PGF2 agent.. The objective was to ascertain whether a pill containing PGF2alpha was of endocrinological consequence in humans, or possible its luteolytic effect had an anatomical substrate. The progesterone production of corpus luteum was significantly reduced by 20 mg of PGF2alpha placed in the cervix of primigravidas in 7-8 weeks of pregnancy possible indicative of the site of the anatomical substrate of PGF2alpha action. Case 1: Adjacent to the uterus of a tertigravida aged 25 at 7-8 weeks of gestation was a mobile, flexible, smooth, and not sensitive formation the size of a goose egg. Case 2: a secundigravida of aged 29 at 8-9 weeks of pregnancy. In front of the uterus of was a smooth-surfaced, flexible, mobile, not sensitive formation. Following evacuation of the uterus, laparotomy was performed in both cases after insertion of an Enzaprost-F pill (20 mg of PGF2alpha) into the cervix. Blood tests were taken prior to and 2, 4, and 6 hours after treatment for the determination of progesterone and 17-beta-estradiol levels by radioimmunoassay. Results of Case 1: an ovarial sac with corpus luteum was found, and the right adnexa was removed. Results of Case 2: also during laparotomy a cyst was detected on the right side with corpus luteum in it. Right adnexectomy was performed. Dilatation of the cervix with 20 mg of PGF2alpha: 8 mm 2 hours after and 13 mm (Hegar 13) 4 1/2 hours after insertion. Serum progesterone level was 34.7 nmol/1 prior to treatment and at 2, 4, and 6 hours afterwards it was 31.9 and 28.9 nmol/1. The respective serum 17-beta-estradiol levels increased from 7.656 nmol/1 to 7.630, 8.074, and 7.896 nmol/1. Histological examination indicated initial signs of a hypertrophic colloid-cystic aberration of the corpus luteum presumably through the influence of PGF2alpha. A lack of significant decrease of serum steroid concentration values also substantiate this supposition.

Topics: Abortion, Therapeutic; Administration, Topical; Adult; Corpus Luteum; Dilatation and Curettage; Dinoprost; Female; Humans; Ovarian Cysts; Pregnancy; Pregnancy Complications; Preoperative Care; Tablets

In 160 cows with ovarian cysts as determined by rectal palpation, differentiation was made of follicular cyst, luteal cyst, and cystic corpus luteum on the basis of milk progesterone concentrations estimated by an enzyme immunoassay before and at 10 days after cows were treated with gonadotropin-releasing hormone. Cows having a progesterone concentration in skim milk less than 1.0 ng/ml were considered to have follicular cysts and those with concentrations of 1.0 ng/ml or higher were regarded as the cases of luteal cyst or cystic corpus luteum. Luteal cyst was characterized by progesterone values remaining high in the cows for 10 days after treatment, and cystic corpus luteum was characterized by a decrease in progesterone concentration after cows were treated. By the rectal palpation procedure it was impossible to differentiate luteal cyst and cystic corpus luteum from follicular cyst. The frequencies of follicular cyst, luteal cyst, and cystic corpus luteum were 65%, 19%, and 16%, respectively. Of 104 cows with follicular cysts as defined by milk progesterone assay result, 73 (70%) responded to the treatment with gonadotropin-releasing hormone, the milk progesterone concentration increasing from 0.7 +/- 0.2 ng/ml (mean +/- SD) to 1.8 +/- 1.1 ng/ml. The accuracy of rectal palpation 10 days after treatment for judgment of luteinization of follicular cyst confirmed by milk progesterone analysis was only 30% (48 cows of 160).

Topics: Animals; Cattle; Cattle Diseases; Chorionic Gonadotropin; Corpus Luteum Maintenance; Diagnosis, Differential; Dinoprost; Female; Immunoenzyme Techniques; Milk; Ovarian Cysts; Palpation; Pituitary Hormone-Releasing Hormones; Pregnancy; Progesterone; Prostaglandins F

Ten veterinarians diagnosed and treated 296 cows for reproductive irregularities on 28 dairy farms. Average interval from treatment with 25 mg of prostaglandin F2 alpha (137 cows) to estrus for cows with a corpus luteum (14.6 days) was reduced compared to saline (52 cows) (21.8 days), and a greater percentage (41.6 versus 17.3%) showed estrus within 6 days of treatment. Cows with a cystic corpus luteum (28) were detected in estrus at comparable intervals after prostaglandin F2 alpha or saline (21.8 versus 21.2 days), and similar proportions of cows were detected in estrus within 6 days of treatment (16.7 versus 10.0%). Intervals to estrus among cows with cystic follicles (40 cows) or anestrous cows with follicular ovaries (39 cows) did not differ after treatment with saline or 100 or 200 micrograms of gonadotropin releasing hormone. Within 25 days after treatment, 47.5% of cows with cystic ovaries and 64.1% of anestrous cows showed estrus. Number of cows pregnant after as many as three artificial inseminations of the number examined was 46%, whereas average number of conceptions per service was .32. The long interval from parturition to examination (112 days), the low rate of estrous response after treatment, and the long interval (1.6 estrous cycles) between estruses are consonant with inadequate estrous detection.

Topics: Anestrus; Animals; Cattle; Corpus Luteum; Dinoprost; Estrus; Female; Fertility; Gonadotropin-Releasing Hormone; Ovarian Cysts; Pregnancy; Prostaglandins F

Topics: Animals; Dinoprost; Estradiol; Female; Hormones; Lactation; Luteinizing Hormone; Ovarian Cysts; Pregnancy; Progesterone; Prostaglandins F; Puerperal Disorders; Swine; Swine Diseases