dinoprost and Lyme-Disease

The objective of the study was measurement of serum arachidonic acid level as well as the product of its peroxidation - 8-isoPGF2α, and the activity of phospholipase A2 and PAF-acetylhydrolase that participate in releasing 8-isoPGF2α from glycerol skeleton, and the potential designation of their role in the pathomechanism of Lyme disease (LD).. Changes in the phospholipid arachidonic acid level and the level of 8-isoPGF2α were determined in the plasma and urine of patients with LA (n=57) and of healthy controls (n=41). The activity of phospholipase A2 and PAF acetylhydrolase were assayed. All examined parameters were also measured in the plasma of some LA patients (n=13) after antibiotics treatment.. An almost 3-fold higher level of the total plasma 8-isoPGF2α was observed in LA patients compared to the controls, while in the urine it increased over 5-fold. The plasma PLA2 activity was more than 3-fold higher in LA patients than in the healthy subjects, while PAF acetylhydrolase activity was observed to be modestly, but not significantly lower. The total 8-isoPGF2α level in the plasma and urine of LA patients was significantly lower after antibiotics treatment. The plasma activity of PAF-AH in the LA patients was increased, while the cPLA2 activity decreased after antibiotics treatment.. It may be suggested that in the course of LA, the level of binding 8-isoPGF2α is significantly enhanced, and it may also be suggested that uncontrolled changes in the lipid status of some patients may make their Lyme arthritis unresponsive to antibiotics.

Topics: 1-Alkyl-2-acetylglycerophosphocholine Esterase; Adult; Aged; Arachidonic Acid; Dinoprost; Female; Humans; Lyme Disease; Male; Middle Aged; Oxidation-Reduction; Phospholipases A2; Poland; Young Adult

Lipid peroxidation products, malondialdehyde (MDA), 4-hydroxynonenal (4-HNE) and [Formula: see text], were determined in the plasma and urine of patients with Lyme arthritis and healthy people. The group consisted of 19 patients with Lyme arthritis (mean age 47 years) and the control group consisted of 16 healthy individuals (mean age 38 years). Diagnosis of Lyme disease was confirmed by epidemiological anamnesis, clinical manifestation of arthritis and serological examinations. Lipid peroxidation was estimated by the measurement of aldehydes (MDA and 4-HNE, determined by high-performance liquid chromatography [HPLC]) and prostaglandin derivatives (8 - isoPGF(2a), determined by liquid chromatography/mass spectrometry [LC/MS]). MDA and 4-HNE levels were increased about 2-4-fold in the plasma, while in the urine, the increases were about 2-fold. More significant increases were noted for the 8 - isoPGF(2a) total plasma level, which was enhanced over 4-fold, and for the urine 8 - isoPGF(2a) level, which was increased over 8-fold. The 8 - isoPGF(2a) total plasma level consists of free and esterified form. During infection, the ratio of free to esterified form is significantly smaller compared to healthy people. The ratio of free to esterified form of 8 - isoPGF(2a) may be a useful indicator of Lyme arthritis. Moreover, the complementarities of three lipid peroxidation product levels may be helpful in the diagnosis of Lyme arthritis.

Topics: Adult; Aged; Aldehydes; Antibodies, Bacterial; Blotting, Western; Borrelia burgdorferi; Chromatography, High Pressure Liquid; Chromatography, Liquid; Dinoprost; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunoglobulin G; Immunoglobulin M; Lipid Peroxidation; Lyme Disease; Male; Malondialdehyde; Mass Spectrometry; Middle Aged