dinoprost and Infertility

Previous studies have indicated that OxS (oxidative stress) may appear as a possible reason for poor ART outcome. Our aim was to study OxS levels in both partners of couples seeking Assisted reproduction Technology (ART).. Altogether 79 couples were recruited. Oxidative DNA damage (8-OHdG) and lipid peroxidation (8-EPI) were measured, and clinical background and ART outcomes were recorded.. Both OxS markers accurately reflected clinical conditions with prominent negative effects attributable to genital tract infections, endometriosis, uterine myoma and smoking. Furthermore, the level of OxS was also affected by partner's state of health. The highest 8-EPI levels were detected in both partners when biochemically detectable pregnancies did not develop into clinically detectable pregnancies (in women, 97,8 ± 16,7 vs 72.9 ± 22,9, p = 0.007; in men, 89.6 ± 20,4 vs 72,1 ± 22,6, p = 0.049).. To conclude, high grade systemix OxS in both partners may negatively affect the maintenance and outcome of pregnancy. Applying the detection of OxS in ART patients may select patients with higher success rate and/or those who require antioxidant therapy. This would lead to improvement of ART outcome as well as natural fertility.

Topics: Adult; Biomarkers; Dinoprost; DNA Damage; Female; Fertilization in Vitro; Humans; Infertility; Lipid Peroxidation; Male; Oxidative Stress; Pregnancy; Pregnancy Outcome; Reproductive Techniques, Assisted; Semen Analysis; Urinalysis

To test for differences in the amount and activity of peritoneal macrophages present in the peritoneal fluid of women with, and without endometriosis using prostaglandin release by macrophages in culture as a marker.. Women of reproductive age undergoing laparoscopy for infertility or chronic pelvic pain with postoperative diagnosis of endometriosis and women undergoing laparoscopy for sterilization.. Peritoneal fluid was aspirated during laparascopy, volume was recorded, macrophages were isolated via a Ficoll Paque gradient and kept in primary culture. PGE2 and PGF2 alpha release of the cells were measured before and after stimulation with zymosan.. Women with endometriosis had significantly more peritoneal macrophages than controls. Peritoneal macrophages of women with endometriosis released significantly more PGE2 than those of the control group: 8.4 +/- 2.0 versus 1.4 +/- 0.4 ng/ml/10(6) cells (mean +/- SEM, p = 0.0005) and PGF2 alpha: 10 +/- 4.3 (endometriosis) versus 1.8 +/- 0.4 (control) ng/ml/10(6) cells (mean +/- SEM, p = 0.045).. There is a significant increase in the amount of prostaglandins released by peritoneal macrophages from women with endometriosis. These prostaglandins might alter uterine and tubal contractility, thereby affecting fertility.

Topics: 6-Ketoprostaglandin F1 alpha; Adult; Ascitic Fluid; Dinoprost; Dinoprostone; Endometriosis; Extracellular Space; Female; Humans; Infertility; Macrophages, Peritoneal; Thromboxane B2

Peritoneal fluid from sterile women where the state of the pelvis is changed by endometriosis, inflammation, infection, and in certain cases where it appears normal laparoscopically, can immobilise spermatozoa in minutes or hours. This toxicity against male gametes could be an explanation of the mechanism for such sterility. The tubes and the ovaries are bathed in this liquid in the periovulatory phase when they are largest in size. If these first observations are confirmed fully many theoretical, practical and therapeutic implications must follow.

Topics: Amenorrhea; Body Fluids; Dinoprost; Endometriosis; Female; Humans; Immobilization; Infertility; Male; Pelvic Inflammatory Disease; Peritoneal Cavity; Prostaglandins E; Prostaglandins F; Sperm Motility; Sterilization, Reproductive