dinoprost and Infertility--Male

The effects of 24 weeks of high-intensity interval training (HIIT) on markers of male reproductive function in infertile patients were studied. Infertile men (n = 441) were randomized to exercise (EX, n = 221) or non-exercise (NON-EX, n = 220) group. Patients in the EX group performed an interval training (1:1 work:rest ratio) 3 times per week at 75-95% of maximal oxygen consumption, for 24 weeks (VO

Topics: Adult; Antioxidants; Correlation of Data; Cytokines; Dinoprost; Exercise Therapy; Female; High-Intensity Interval Training; Humans; Infertility, Male; Inflammation; Interleukin-1beta; Interleukin-6; Interleukin-8; Male; Oxidative Stress; Oxygen Consumption; Pregnancy; Reactive Oxygen Species; Semen; Spermatozoa; Tumor Necrosis Factor-alpha

This study investigated for the first time the changes in seminal markers of inflammation, oxidative stress status, semen parameters, sperm DNA integrity as well as pregnancy rate following 24weeks of moderate aerobic exercise in infertile patients. A total of 1026 sedentary men (aged 25-40years) attending the infertility clinic with history of more than one year of infertility, were screened and 419 were randomized to either exercise (EX, n=210) or non-exercise (NON-EX, n=209) groups. Exercise training favorably attenuated seminal markers of both inflammation (IL-1β, IL-6, IL-8, and TNF-α) and oxidative stress (ROS, MDA, 8-Isoprostane) as well as enhanced antioxidant defense system (SOD, catalase and TAC) (P<0.05). These changes correlate with favorable improvements in semen parameters, sperm DNA integrity and pregnancy rate (P<0.05). The results provide information about the effectiveness of moderate aerobic exercise training as a treatment option for male factor infertility. The 4-week detraining period was not enough to reverse all benefits promoted by exercise intervention.

Topics: Adult; Catalase; Cytokines; Dinoprost; Exercise; Exercise Therapy; Humans; Infertility, Male; Male; Malondialdehyde; Reactive Oxygen Species; Superoxide Dismutase

Low seminal plasma concentrations of coenzyme Q10 (CoQ10) have been correlated with impaired sperm parameters, but the exact mechanism remains of dominating interest. This randomised, placebo-controlled study examined the effect of CoQ10 on catalase, superoxide dismutase (SOD) and F2 -isoprostanes in seminal plasma in infertile men and their relation with CoQ10 concentration. Sixty infertile men with idiopathic oligoasthenoteratozoospermia (OAT) were randomised to receive 200 mg d(-1) of CoQ10 or placebo for 3 months. 47 persons of them completed the study. Semen analysis, anthropometric measurements, diet and physical activity assessment were performed for subjects before and after treatment. Independent and paired t-test, chi-square test and ancova were compared outcomes of supplementation between two groups. CoQ10 levels increased from 44.74 ± 36.47 to 68.17 ± 42.41 ng ml(-1) following supplementation in CoQ10 (P < 0.001). CoQ10 group had higher catalase and SOD activity than the placebo group. There was a significant positive correlation between CoQ10 concentration and normal sperm morphology (P = 0.037), catalase (P = 0.041) and SOD (P < 0.001). Significant difference was shown between the mean of changes in seminal plasma 8-isoprostane in two groups (P = 0.003) after supplementation. Three-month supplementation with CoQ10 in OAT infertile men can attenuate oxidative stress in seminal plasma and improve semen parameters and antioxidant enzymes activity.

Topics: Adult; Antioxidants; Catalase; Dinoprost; Double-Blind Method; Humans; Infertility, Male; Male; Oxidative Stress; Semen; Sperm Motility; Superoxide Dismutase; Ubiquinone

Polyunsaturated fatty acid damages lead to alterations in sperm function. This study aimed to investigate the involvement of F

Topics: Adult; Dinoprost; F2-Isoprostanes; Humans; Immunohistochemistry; Infertility, Male; Male; Semen; Spermatozoa; Varicocele

Progesterone, prostaglandin and follicular fluid are reported to enhance the acrosome reaction through the influx of extracellular calcium into the cytoplasm of human spermatozoa. Prostaglandins are present within the male reproductive tract, and high concentrations of prostaglandins exist in seminal fluid. In order to investigate the mechanisms by which prostaglandins enhance the acrosome reaction through calcium influx, the intracellular calcium response induced by progesterone, prostaglandin E1 (PGE1), prostaglandin E2 (PGE2) and follicular fluid was measured using fura-2. PGE1 and PGE2 promoted calcium influx dose dependently through dihydropyridine insensitive calcium channels. Refractoriness of the elevation of intracellular Ca2+ concentration ([Ca2+]i) to a second stimulus occurred when 60 microg/ml PGE1 was administered 100 s after the prior administration of 60 microg/ml of PGE1, and similarly when 1 microg/ml of progesterone was administered 100 s after the prior administration of 1 microg/ml of progesterone. Refractoriness also occurred when 60 microg/ml PGE1 was administered after the prior addition of 60 microg/ml PGE2, but did not occur between PGE1 and progesterone. Pertussis toxin (PTX) did not modify the changes in [Ca2+]i after the addition of PGE1 or PGE2. In conclusion, PGE1 and PGE2 promoted calcium influx through PTX-insensitive calcium channels which appeared to be recognized by a common receptor different from that of progesterone.

Topics: Acrosome; Adult; Alprostadil; Angiotensin II; Atrial Natriuretic Factor; Body Fluids; Calcium; Calcium Channel Blockers; Calcium Channels; Culture Media; Cyclic AMP; Dihydropyridines; Dinoprost; Dinoprostone; Drug Interactions; Endothelin-1; Female; Humans; Infertility, Male; Ion Transport; Male; Ovarian Follicle; Pentoxifylline; Pertussis Toxin; Progesterone; Sperm Capacitation; Sperm-Ovum Interactions; Spermatozoa; Virulence Factors, Bordetella

The levels of 19-hydroxy-prostaglandins F1 alpha/F2 alpha (PG F) in the semen of 19 vasectomized, 44 infertile and 8 fertile men were determined using a simple RIA technique. The mean concentrations observed in this survey were 45 micrograms/ml, 49.5 micrograms/ml and 59 micrograms/ml, respectively. No significant difference was recorded between the vasectomized and infertile groups; there were too few fertile samples available to undertake a meaningful statistical comparison. No reduction was observed in the levels of this PG in a liquid semen sample retained at room temperature over a 4 week period in the presence of a bacteriostat (sodium azide). However, a 30% reduction in the levels of 19-OH PG F occurred over the same time period when aliquots of the same semen sample were retained at either room temperature or at 4 degrees C without azide. Finally, no reduction was observed in the concentration of 19-OH PG F in a series of 10-microliters semen stains stored over a period of 6 weeks at room temperature.

Topics: Dinoprost; Humans; Infertility, Male; Male; Radioimmunoassay; Reference Values; Semen; Staining and Labeling; Vasectomy

The sensitivity of a simple radioimmunoassay (RIA) for the detection of 19-OH prostaglandin F1 alpha/F2 alpha (PG F) has been evaluated on a number of semen samples from vasectomized, infertile and fertile donors. The specificity of the test has been examined by testing saliva, sweat and urine from a number of male and female donors. The assay technique could readily detect the PG in semen stains prepared from 0.2 microliter of semen from normal, infertile and vasectomized donors. The detection limit of the assay system, based on the observed displacement, was calculated to be approximately 0.05 microliter semen. The assay could be conducted over a pH range of 7.5-10.5 even after the PG has been heated to 100 degrees C. The 19-OH series of PG were absent from sweat, saliva and female urine using the normal assay protocol; volumes in excess of 100 microliters of some urines particularly from women in labour and those with acute urinary tract infection showed some displacement. Low levels of PG were detected in 50% of the male urine analysed. However, urine samples from men who had engaged in recent sexual activity contained relatively high concentrations of PG which could be readily detected in 10 microliters of urine. These results emphasise the potential of these compounds as specific and sensitive markers for the presence of human semen.

Topics: Dinoprost; Female; Humans; Infertility, Male; Male; Radioimmunoassay; Reference Values; Saliva; Semen; Staining and Labeling; Sweat; Vaginal Smears; Vasectomy

The contents of prostaglandins in seminal plasma from a total of 73 men were evaluated. The subjects were grouped as follows: normospermic men, patients with impaired motility, patients with small untreated varicocele and patients with impaired motility and Kallikrein therapy. Sperm density, morphology and motility were examined. High performance reversed phase liquid chromatography (HPLC) in combination with specific radioimmunoassays were used for the determination of PGE2, PGI2 and PGF2 alpha. There was a significant difference (p less than 0.025; F-test) between the PGI2 concentrations in patients with impaired motility (5.6 +/- 1.4 pg/mg protein) and normal men (8.8 +/- 3.7 pg/mg protein). PGE2 and PGF2 alpha were significantly different in patients with varicocele (p less than 0.025, F-test). Wide ranges of prostaglandins occurred in the Kallikrein-group with no significant differences. We conclude that: a) PGI2 is an additional prostaglandin compound in seminal plasma, b) its measurement may not be useful as diagnostic parameter in subfertile men and c) Kallikrein has no influence on the prostaglandin content in seminal plasma and other seminal parameters as motility, motility index and sperm counts.

Topics: Dinoprost; Dinoprostone; Epoprostenol; Fructose; Humans; Infertility, Male; Kallikreins; Male; Prolactin; Prostaglandins; Prostaglandins E; Prostaglandins F; Semen; Sperm Count; Sperm Motility; Testosterone

Prostaglandin (PG) F2 alpha and PGE were measured in 163 semen samples from 145 men attending our male infertility clinic. In addition, each semen sample was analyzed for 13 different fertility parameters. Blood plasma levels of testosterone, follicle-stimulating hormone, and luteinizing hormone were also determined in many of the patients. The data obtained were then analyzed using multiple regression analyses on each PG for all of the parameters studied. The results indicate seminal PGF2 alpha and PGE concentrations were 2.78 +/- 0.24 micrograms/ml and 46.0 +/- 4.5 micrograms/ml, respectively. The seminal parameters that were significant predictors of seminal PGF2 alpha included: Ca++ concentration (P less than 0.001), Zn++ concentration (P less than 0.01), and percentage of tapered sperm (P less than 0.05). The seminal parameters that were significant predictors of seminal PGE included: Ca++ concentration (P less than 0.01) and sperm motility (P less than 0.05). Plasma testosterone was also a significant predictor of seminal PGE (P less than 0.05). These results suggest that seminal PGs are important to the human male fertility potential in that their levels are significantly interdependent with specific parameters of male fertility.

Topics: Adult; Dinoprost; Follicle Stimulating Hormone; Humans; Infertility, Male; Luteinizing Hormone; Male; Middle Aged; Prostaglandins; Prostaglandins E; Prostaglandins F; Semen; Testosterone