dinoprost and Cholecystitis

This study examines the hypothesis that PAF stimulates release of PGI2 from inflamed rabbit gallbladder explant cell cultures. New Zealand white rabbits underwent bile duct ligation for 72 h (72 h BDL), or sham operation, Sham and 72 h BDL gallbladder explants were placed in culture, and the cells grown to 75% confluence. The cells were exposed to increasing concentrations of PAF for 60 min. The media analyzed for eicosanoid release by EIA and the cells analyzed for cyclooxygenase and prostacyclin synthase content by immunoblot analysis. PAF increased release of 6-keto-PGF1 alpha from the 72 h BDL gallbladder cell cultures in a dose-related manner which was inhibited by indomethacin preincubation by 90%. The increased 72 h BDL cell release of 6-keto-PGF1 alpha was not associated with changes in the content of cyclooxygenase or prostacyclin synthase. PAF did not alter eicosanoid release from sham control cell cultures. These data suggest that PAF can only up-regulate endogenous 6-keto-PGF1 alpha release from the 72 h BDL cells that had been previously stimulated by inflammation. PAF may thus contribute to gallbladder distention and injury by chronic stimulation of inflamed gallbladder PGI2 release.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Antibodies; Cells, Cultured; Cholecystitis; Cytochrome P-450 Enzyme System; Dinoprost; Dose-Response Relationship, Drug; Epoprostenol; Gallbladder; Immunoblotting; Indomethacin; Intramolecular Oxidoreductases; Isomerases; Platelet Activating Factor; Prostaglandin-Endoperoxide Synthases; Proteins; Rabbits; Thromboxane B2

The profiling of eicosanoids, including prostaglandin E2 (PGE2), prostaglandin F2 alpha (PGF2 alpha), 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha), thromboxane B2 (TXB2) and leukotriene B4 (LTB4), in dog and human gall bladders was carried out by a combination of an effective and convenient clean-up procedure and gas chromatography with selected-ion monitoring. The clean-up procedure was based on the stepwise elution of their methyl ester derivatives from a silica gel column with n-hexane-ethyl acetate and ethyl acetate-methanol in various ratios. The LTB4 methyl ester was eluted with an n-hexane-ethyl acetate (2:1, v/v) fraction because LTB4 is more lipophilic than the other eicosanoids. The present method permitted the quantitation of trace amounts of eicosanoids, including LTB4, present in tissues in the order of pg/mg of protein, without interference from other endogenous substances. In experimental acalculous cholecystitis produced in dog, the levels of eicosanoids (except LTB4) were significantly changed. Of these eicosanoids, the level of 6-keto-PGF1 alpha was significantly higher in the seromuscular layer and correlated with the observed severe morphological changes. In human chronic cholecystitis with gallstones, the level of 6-keto-PGF1 alpha in the mucosal layer was significantly higher than that in the seromuscular layer. These data suggest that prostaglandin I2 may play an important pathophysiological role in the course of cholecystitis.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Cholecystitis; Dinoprost; Dinoprostone; Dogs; Eicosanoids; Female; Gallbladder; Gas Chromatography-Mass Spectrometry; Humans; Leukotriene B4; Male

The bile content of trace elements, prostaglandins and cyclic nucleotides was measured in 66 patients in the early times (from 2 to 3 weeks) after cholecystectomy. In 60 patients, the content of trace elements was measured in blood serum. The above characteristics were found to correlate with the clinical features of the patients. The relationship was also established between blood serum and bile trace elements, between prostaglandins and cyclic nucleotides.

Topics: Adolescent; Adult; Age Factors; Bile; Cholecystectomy; Cholecystitis; Cholelithiasis; Dinoprost; Female; Humans; Male; Middle Aged; Nucleotides, Cyclic; Prostaglandins; Prostaglandins E; Sex Factors; Time Factors; Trace Elements

Topics: Acute Disease; Adult; Cells, Cultured; Cholecystitis; Dinoprost; Escherichia coli; Humans; Interleukin-1; Lipopolysaccharides; Middle Aged; Monocytes; Prostaglandins E; Prostaglandins F