digoxigenin has been researched along with Sensitivity and Specificity in 209 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 1 (0.48) | 18.7374 |
| 1990's | 147 (70.33) | 18.2507 |
| 2000's | 49 (23.44) | 29.6817 |
| 2010's | 11 (5.26) | 24.3611 |
| 2020's | 1 (0.48) | 2.80 |
| Authors | Studies |
|---|---|
| Hwang, SH; Kim, SK; Ko, DH; Oh, HB; Oh, YH; Sung, H | 1 |
| Deng, Z; Han, Y; Meng, X; Su, S; Wu, Y; Yang, R | 1 |
| Gong, P; Li, H; Li, J; Wu, W; Yang, J; Yang, Z; Zhang, G; Zhang, X | 1 |
| Lai, TP; Shay, JW; Wright, WE | 1 |
| Christopoulos, TK; Ioannou, PC; Konstantou, JK | 1 |
| Girisha, SK; Karunasagar, I; Khushiramani, R | 1 |
| Basu, C; Hart, SM | 1 |
| Berensmeier, S; Gold, A; Schwartz, T; Stankiewicz, N; Yüksel, Y | 1 |
| Coleman, D; Fox, M; Good, J; Jiang, J; Lewin-Koh, SC; Lowe, J; Peng, K; Qiu, ZJ; Quarmby, V; Rahman, A; Song, A; Yang, J; Ying, Y | 1 |
| Alegret, S; Campoy, S; Fabiano, S; García, MI; Lermo, A; Liébana, S; Pividori, MI; Soutullo, A; Zumárraga, MJ | 1 |
| Fischer, SK; Peng, K; Quarmby, V; Siradze, K | 1 |
| Hengartner, CJ; Ortega, LM; Vega, LR | 1 |
| Roth, J | 1 |
| Colao, V; Di Pinto, A; Di Pinto, P; Tantillo, G; Terio, V | 1 |
| Ang, WH; Chan, Y; Ho, HK; Pastorin, G; Tian, Q; Wong, W; Xu, Y | 1 |
| Lu, C; Niu, C; Wang, S | 1 |
| Anderson, SA; Lee, M; Sissons, CH; Wall-Manning, GM | 1 |
| Guesdon, JL; Mouratou, B; Pauillac, S; Rouyre, S | 1 |
| Gutstein, HB | 1 |
| Bastert, G; Eichler, A; Kaul, S; Zhong, X | 1 |
| Bast, RC; Hoque, A; Lippman, SM; Liu, J; Lu, Z; Luo, RZ; Nishimoto, A; Sahin, AA; Wang, L; Yu, Y; Yuan, J | 1 |
| Kim, O | 1 |
| Bouwman, K; Costa, J; Dillon, D; Haab, BB; Lizardi, P; Marrero, JA; Schotanus, M; Verweij, C; Zhou, H | 1 |
| Blais, BW; Gauthier, M | 1 |
| Chen, YX; Guo, SH; Huang, AL; Qi, ZY | 1 |
| Barros-Núñez, P; Jaloma-Cruz, AR; Leal, E | 1 |
| Bischoff, S; Fromme, M; Gromnica-Ihle, E; Gross, J; Haselbeck, A; Hoesel, W; Kanne, B; Moller, R; Müller, A; Müller, G; Wessner, A | 1 |
| Bower, SM; Carnegie, RB; Meyer, GR | 1 |
| Fitzgibbon, JE; Sagripanti, JL | 1 |
| Kumarasinghe, G; Kurupati, P; Laa Poh, C | 1 |
| Eblen, BS; Ferreira, JL; Sharma, SK; Whiting, RC | 1 |
| Bitensky, MW; Gifford, SC; Shevkoplyas, SS; Yoshida, T | 1 |
| Chen, YF; Hsieh, JS; Huang, SY; Huang, TJ; Lin, SR; Wang, JY; Wu, CH; Yeh, CS | 1 |
| Bisucci, T; Darby, IA; Desmoulière, A; Hewitson, TD | 1 |
| Chotteau-Lelièvre, A; Dollé, P; Gofflot, F | 1 |
| Choi, C; Hudson, LG; Kusewitt, DF; Savagner, P | 1 |
| Amiri, MV; Eshraghi, N; Ghaemi, A; Ghalami, M; Gill, P; Hashempour, T; Ramezani, R; Tehrani, HA | 1 |
| Forouzandeh, M; Gill, P; Rahbarizadeh, F; Ramezani, R; Rasaee, MJ | 1 |
| Alain, S; Ducancelle, A; Mazeron, MC; Petit, F; Sanson Le Pors, MJ | 1 |
| Fragner, K; Ladinig, A; Mrakovcic, M; Weissenböck, H | 1 |
| Liu, Y; Sun, B; Wang, X; Zheng, C; Zhou, G | 1 |
| Liu, J; Shi, L; Wang, J; Wei, L; Zhou, J | 1 |
| Beutler, AM; Hudson, AP; Salameh, WA; Schumacher, HR; Whittum-Hudson, JA | 1 |
| Krueger, SK; Williams, DE | 1 |
| Hayashi, M; Kamiya, M; Kurosawa, T; Okamoto, M; Oku, Y; Ueda, H | 1 |
| Bottin, MC; Coulais, C; Martinet, N; Rihn, B | 2 |
| Craddock, BP; Kano, T; Okamoto, N; Steigbigel, RT; Suzuki, K | 1 |
| Más, P; Pallás, V; Sánchez-Navarro, JA; Sánchez-Pina, MA | 1 |
| Chang, GJ; Kinney, RM; Mitchell, CJ; Trent, DW; Vergne, E; Vorndam, AV | 1 |
| Byrn, RA; Yamaguchi, K; Zhang, D | 1 |
| Kessler, C; Reischl, U; Rüger, R | 1 |
| Allan, GM; McMahon, J; McQuaid, S | 1 |
| Chen, CH; Chou, YW; Lee, CY | 1 |
| Brown, T; Cubie, HA; da Silva, C; Duncan, L; Grzybowski, J; Smith, NM | 1 |
| Bloch, B; Le Moine, C | 1 |
| Bartsch, U; Dörries, U; Nolte, C; Roth, J; Schachner, M | 1 |
| Engler-Blum, G; Frank, J; Meier, M; Müller, GA | 1 |
| Craddock, BP; Kano, T; Steigbigel, RT; Suzuki, K | 1 |
| Colgan, DJ | 1 |
| Ferrero, EA; Fitch, MM; Moore, PH; Wu, KK | 1 |
| Bucy, RP; Panoskaltsis-Mortari, A | 1 |
| Singh, M; Singh, RP | 1 |
| Bucy, RP; Devore-Carter, D; Karr, LJ; Li, J; Panoskaltsis-Mortari, A; Weaver, CT | 1 |
| Fujita, S; Lasker, BA; Lott, TJ; Morrison, CJ; Reiss, E | 1 |
| Hayashi, M; Iyama, K; Kitaoka, M; Monda, M; Ninomiya, Y | 1 |
| Miller, JJ; Straub, RW; Valdes, R | 1 |
| Borghi, V; Gentilomi, G; La Placa, M; Musiani, M; Pecorari, M; Pietrosemoli, P; Venturoli, S; Zerbini, M | 1 |
| Gergely, E; Kessler, HH; Marth, E; Pierer, K; Sakrauski, A; Santner, B; Stuenzner, D; Weber, B | 1 |
| Atkins, RC; Endoh, M; Koji, T; Miyazaki, M; Nikolic-Paterson, DJ; Nomoto, Y; Sakai, H | 1 |
| Gas, N; Gleizes, PE; Noaillac-Depeyre, J | 1 |
| Arranz, JA; Buti, M; Esteban, R; Jardí, R; Rodríguez-Frías, F | 1 |
| Gallinella, G; Gentilomi, G; Gibellini, D; La Placa, M; Musiani, M; Venturoli, S; Zerbini, M | 1 |
| Añez, N; Da Silveira, JF; Galindo, I; González, N; Guevara, P; Novak, E; Ramírez, JL; Scorza, JV | 1 |
| Canepari, P; Di Stefano, CF; Lleò, MM | 1 |
| Gentilomi, G; Gibellini, D; La Placa, M; Musiani, M; Venturoli, S; Zerbini, M | 1 |
| Gressens, P; Martin, JR | 1 |
| Chen, DF; Endres, W; Meyer, SA; Stangel, W | 2 |
| Belisle, JT; Norgard, MV; Radolf, JD; Weigel, LM | 1 |
| Ball, JK; King, JA | 1 |
| Viscidi, R; Yang, B; Yolken, R | 1 |
| Müller, C; Vandevelde, M; Zurbriggen, A | 1 |
| Böni, J; Schüpbach, J | 1 |
| Aozasa, K; Hirota, S; Horiuchi, K; Kitamura, Y; Mishima, K; Nomura, S; Saeki, K | 1 |
| Beauvillain, JC; Gambiez, A; Mitchell, V | 1 |
| Agrawal, S; Kubert, M; Temsamani, J | 1 |
| Duggleby, CJ; Hutson, RA; Lowe, JR; Manchee, RJ; Turnbull, PC | 1 |
| Begum, D; Jackson, MP; Sowers, EG; Strockbine, NA | 1 |
| Azzi, A; Ciappi, S; Zakrzewska, K | 1 |
| Akin, A; Keirs, RW; Lin, TL; Wu, CC | 1 |
| Bombara, MP; Espevik, T; Laegreid, A; Liabakk, NB; Lien, E; Ranges, G; Sundan, A | 1 |
| Clarridge, JE; el-Zaatari, FA; Nataraj, A; Shawar, RM | 1 |
| Bobrow, LG; Happerfield, LC; Isaacson, PG; Pan, L | 1 |
| Brunings, HA; Goverde, RL; Huis in 't Veld, JH; Jansen, WH; Mooi, FR | 1 |
| Holmstrøm, K; Rasmussen, OF; Rossen, L | 1 |
| Kano, T; Okamoto, N; Suzuki, K | 1 |
| Karl, DM; Landry, MR; Selph, KE | 1 |
| DeFiglia, JA; Dubitsky, A | 1 |
| Gallinella, G; Gentilomi, G; Gibellini, D; Musiani, M; Venturoli, S; Zerbini, M | 1 |
| Daha, MR; Raap, AK; Tanke, HJ; van Gijlswijk, RP; van Gijlswijk-Janssen, DJ | 1 |
| Blouin, EF; Ge, NL; Kocan, KM; Murphy, GL | 1 |
| Dessaux, Y; Elasri, M; Glickmann, E; Oger, P; Petit, A; Vaudequin-Dransart, V | 1 |
| André, PM; Clossais Besnard, N; Colimon, R; Gicquel, J; Minjolle, S | 1 |
| Delattre, J; Hainault, I; Hainque, B; Hajduch, E; Lavau, M; Nivet, V | 1 |
| Lefrère, JJ; Mariotti, M | 1 |
| Blaschke, AJ; Chun, J; Staley, K | 1 |
| DiRienzo, JM; Lai, CH; Listgarten, MA; Wong, M | 1 |
| Purschke, WG; Schäfer, G | 1 |
| Hassoun, PM; Lanzillo, JJ; Stevens, J; Yu, FS | 1 |
| Dessi, JL; Self, CH; Winger, LA | 1 |
| Fonseca, ME; Gander, E; Marcellino, LH | 1 |
| Cho, SW; Han, DC; Hwang, SG; Jin, SY; Lee, DW; Lee, HB; Lee, MS; Shim, CS | 1 |
| Barisić, K; Flögel, M; Lauc, G; Zanić, T | 1 |
| Ciesielska, U; Wysocka, T; Zabel, M | 1 |
| Villarreal, LP; You, Z | 1 |
| Gorrell, RJ; Palombo, EA | 1 |
| Jin, L; Lloyd, RV; Sperry, A | 1 |
| Lahaye, T; Rueger, B; Schulze-Lefert, P; Thalhammer, J; Toepsch, S | 1 |
| Hakoda, E; Ikezoe, T; Kobayashi, M; Manabe, T; Miyagi, S; Miyoshi, I; Ohtsuki, Y; Sonobe, H; Uemura, Y; Urata, T | 1 |
| Fahimi, HD; Vöelkl, A; Zhan, J | 1 |
| Albert, ED; Nevinny-Stickel, C | 1 |
| Grünweller, A; Müller, PK | 1 |
| D'Antuono, A; Gallinella, G; Gentilomi, G; La Placa, M; Manaresi, E; Musiani, M; Pasini, P; Roda, A; Venturoli, S; Zerbini, M | 1 |
| Chu, CM; Liaw, YF; Sheen, IS; Shyu, WC; Yeh, CT | 1 |
| Linhardt, RJ; Nadkarni, VD | 1 |
| Fuentes, E; Rodríguez, JC; Royo, G | 1 |
| Dancil, KP; Ghadiri, MR; Lin, VS; Motesharei, K; Sailor, MJ | 1 |
| Cabrita, J; Mégraud, F; Monteiro, L | 1 |
| Conrads, G; Mutters, R; Pelz, K; Seyfarth, I | 1 |
| Dahlén, G; Madianos, PN; Papapanou, PN; Sandros, J | 1 |
| Bäckström, B; Edholm, LE; Johansson, G; Kronkvist, K; Lövgren, U | 1 |
| Hirata, K; Kameshima, H; Kikuchi, K; Matsuura, A; Sasaki, K; Sato, N; Uehara, N; Wada, Y; Watanabe, N; Yagihashi, A; Yajima, T | 1 |
| Escrich, E; Solanas, M | 1 |
| Cantoni, C; Cocolin, L; Comi, G; Manzano, M | 1 |
| Du, Y; Pong, X; Wang, Z; Wu, H; Wu, J; Xie, H; Xie, M; Zhang, X | 1 |
| Grino, M; Zamora, AJ | 1 |
| Hopman, AH; Ramaekers, FC; Speel, EJ | 1 |
| Arnal, C; Besse, B; Billaudel, S; Ferré-Aubineau, V | 1 |
| Das, KC; White, CW | 1 |
| Hataya, T; Hayashi, Y; Kimura, I; Nakahara, K; Shikata, E; Sugimoto, T | 1 |
| Durisin, MD; Griffiths, MW; Ibrahim, A | 1 |
| Darby, IA; Fuller, PJ; Wolvekamp, MC | 1 |
| Elie, CM; Lott, TJ; Morrison, CJ; Reiss, E | 1 |
| Broeren, CP; Damoiseaux, JG; Duijvestijn, AM; Theunissen, R; van Breda Vriesman, PJ | 1 |
| Nuovo, GJ | 1 |
| Liu, MT; Wang, SS; Zhu, TF | 1 |
| Krzeszowiak, A; Pituch-Noworolska, A; Ruggiero, I; Wieckiewicz, J; Zembala, M | 1 |
| Obermaier, I; Peterhaensel, C; Rueger, B | 1 |
| Laengin, T; Reske-Kunz, AB; Ross, R; Ross, XL; Rueger, B | 1 |
| Dimulescu, IM; Rajeevan, MS; Unger, ER; Vernon, SD | 1 |
| Callens, M; De Clercq, K | 1 |
| Cook, DW; DePaola, A; Hill, WE; Kaysner, CA; McCarthy, SA | 1 |
| Fernández, A; García, T; González, I; Hernández, PE; Martín, R; Sanz, B | 1 |
| Liu, ML; Thompson, AR | 1 |
| Lennon, A; Riggio, MP | 1 |
| Donzé, O; Maggiolini, M; Picard, D | 1 |
| Hadidi, A; Shamloul, AM | 1 |
| Gage, WR; Kadkol, SS; Pasternack, GR | 1 |
| Markham, RJ; Sanchez, JG; Speare, DJ | 1 |
| Mabuchi, M; MacDonald, CG; Mansfield, MA; Pluskal, MG | 1 |
| Bodepudi, V; Cain, CA; Collarini, EJ; Davidson, S; Davoudzadeh, D; Lee, YJ; Luehrsen, KR; Madlansacay, MR; Magee, K; Mahoney, W; Pearson, J; Raich, T; Rouhani, R; Schueler, PA; Soleimani, A; Yamanishi, DT | 1 |
| Bartram, CR; Janssen, JW; Nakao, M; Schäfer, M | 1 |
| Leise, R; Neumann, U; Ryll, M; Salisch, H | 1 |
| Arseniev, L; Ganser, A; Kadar, J; Lichtinghagen, R; Petersen, D; Trummer, A | 1 |
| Härkönen, P; Toppari, J; Yan, W; Zheng, H | 1 |
| Palmes-Saloma, C; Saloma, C | 1 |
| Amaral, DG; Jongen-Rêlo, AL | 1 |
| Chauhan, DS; Dinesh, DS; Gupta, AK; Kar, SK; Katoch, VM; Kishore, K; Palit, A; Sharma, VD; Singh, D; Verma, N | 1 |
| Gnarpe, J; Lindquist, L | 1 |
| Xian, CJ; Zhou, XF | 1 |
| Morozov, I; Paul, PS; Sirinarumitr, T; Sorden, SD | 1 |
| Bonvicini, F; Cricca, M; Gentilomi, G; Manaresi, E; Musiani, M; Zerbini, M | 1 |
| Calciano, M; Christian, MW; Hamann, S; Leamon, JH; Lizardi, PM; Strugnell, T; Zhou, Y | 1 |
| Bonvicini, F; Cricca, M; Gallinella, G; Gentilomi, G; Musiani, M; Venturoli, S; Zerbini, M | 1 |
| Broketa, M; Drazenović, V; Mlinarić-Galinović, G; Sim, R; Vince, A | 1 |
| Lipart, C; Renault, T | 1 |
| Chang, TT; Chen, SH; Cheng, PN; Hsiao, WC; Jen, CM; Young, KC | 1 |
| Long, S; Rebagliati, M | 1 |
| Branny, P; Hercík, K; Janecek, J; Melter, O | 1 |
| DiPaolo, B; Ji, X; Lee, K | 1 |
| Komminoth, P | 1 |
| Alvarez, JA; Buening, GM; Figueroa, JV; Hernandez, R; Ramos, JA; Rodriguez, RI; Solis, J; Vega, CA | 1 |
| Oskoui, KB; Roth, WK; Stürzl, M | 1 |
| Hartskeerl, RA; Hermans, PW; Kolk, AH; Kuijper, S; Schuitema, AR; van Embden, JD; van Leeuwen, J | 1 |
| Amouriaux, P; Baranton, G; Mérien, F; Perolat, P; Saint Girons, I | 1 |
| An, SF; Fleming, KA; Franklin, D | 1 |
| Jackson, MP | 1 |
| Raap, AK; Tanke, HJ; van Gijlswijk, RP | 1 |
| Hiruki, C; Welnicki, M | 1 |
| Bhan, MK; Bright, BD; Jailkhani, B; Kumar, R; Srivastava, R | 1 |
| Jackson, AC | 1 |
| Crabb, ID; Hicks, DG; Hughes, SS; Puzas, JE; Rosier, RN; Tsao, GJ | 1 |
| Ferreira, MO; Pereira, JM; Taveira, NC | 1 |
| Arnold, N; Kessler, C; Seibl, R; Wienberg, J | 1 |
| Devine, PL | 1 |
| Evans, M; Fleming, KA; Franklin, D; Lovell-Badge, RH; Morey, AL; Ryley, KC | 1 |
| Corbitt, G; Kimpton, CP; Morris, DJ | 2 |
| Morinet, F; Paper, T; Prato, C | 1 |
| Ferri, E; Gallinella, G; Gentilomi, G; Gibellini, D; Girotti, S; Musiani, M; Venturoli, S; Zerbini, M | 1 |
| Arranz, JA; Buti, M; Casacuberta, JM; Esteban, R; Guardia, J; Jardi, R; Puigdomenech, P; Rodriguez-Frias, F; Sansegundo, B | 1 |
| Capelli, A; Larocca, LM; Lauriola, L; Maggiano, N; Piantelli, M; Ranelletti, FO; Ricci, R | 1 |
| Crampton, JM; Hill, SM; Urwin, R | 1 |
| Cassaday, PK; Holloway, BP; Kim, C; Mayer, LW; Swaminathan, B | 1 |
| Alexander, GJ; Daniels, HM; Lau, JY; Naoumov, NV; Williams, R | 1 |
| Bowden, DS; Guo, KJ | 1 |
| Kessler, C; Schmitz, GG; Seibl, R; Walter, T | 1 |
| Archard, LC; Cox, N; Johnston, J; Szarka, Z; Wright, DJ | 1 |
6 review(s) available for digoxigenin and Sensitivity and Specificity
| Article | Year |
|---|---|
Lectins for histochemical demonstration of glycans.
Topics: Digoxigenin; Gold Colloid; Histocytochemistry; Lectins; Microscopy, Electron; Polysaccharides; Sensitivity and Specificity; Staining and Labeling; Tissue Embedding; Tissue Fixation | 2011 |
High sensitivity of chemiluminescent methodology for detection of clonal CDR3 sequences in patients with acute lymphoblastic leukemia.
Topics: Alleles; Antibodies, Monoclonal; Bone Marrow Cells; Burkitt Lymphoma; Chromosome Aberrations; Complement System Proteins; Complementarity Determining Regions; Consensus Sequence; Cytogenetics; Digoxigenin; DNA; Flow Cytometry; Gene Rearrangement; Humans; Immunoglobulin Heavy Chains; Luminescent Measurements; Prognosis; Sensitivity and Specificity | 2004 |
In situ hybridization using cRNA probes: isotopic and nonisotopic detection methods.
Topics: Animals; Autoradiography; Biotin; Digoxigenin; Humans; In Situ Hybridization; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; RNA, Complementary; RNA, Messenger; Sensitivity and Specificity; Tyramine | 2006 |
Expression analysis of murine genes using in situ hybridization with radioactive and nonradioactively labeled RNA probes.
Topics: Animals; Digoxigenin; Embryo, Mammalian; Female; Frozen Sections; Gene Expression; Histological Techniques; In Situ Hybridization; In Vitro Techniques; Mice; Paraffin Embedding; Pregnancy; RNA Probes; Sensitivity and Specificity | 2006 |
An in situ hybridization technique to detect low-abundance slug mRNA in adherent cultured cells.
Topics: Cell Adhesion; Cell Culture Techniques; Cell Line; Digoxigenin; Humans; Immunohistochemistry; In Situ Hybridization; Keratinocytes; RNA Probes; RNA, Messenger; Sensitivity and Specificity; Snail Family Transcription Factors; Transcription Factors | 2006 |
In situ hybridization-theory and practice.
Topics: Animals; Autoradiography; Biotinylation; Buffers; Digoxigenin; DNA; DNA Probes; Fixatives; Fluorescein-5-isothiocyanate; Formaldehyde; Horseradish Peroxidase; Humans; In Situ Hybridization; Isotope Labeling; Microtomy; Oligonucleotide Probes; RNA; RNA Probes; Sensitivity and Specificity; Specimen Handling; Staining and Labeling; Streptavidin; Tissue Fixation | 1999 |
203 other study(ies) available for digoxigenin and Sensitivity and Specificity
| Article | Year |
|---|---|
Nanoparticle-Based Visual Detection of Amplified DNA for Diagnosis of Hepatitis C Virus.
Topics: Biotin; Digoxigenin; DNA; Hepacivirus; Hepatitis C; Humans; Nanoparticles; Polystyrenes; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin | 2022 |
[Detection of Yersinia pestis sRNA by digoxigenin-labeled Northern blot].
Topics: Blotting, Northern; Digoxigenin; Genes, Bacterial; Nucleic Acid Hybridization; Oligonucleotide Probes; RNA Probes; RNA, Bacterial; RNA, Messenger; Sensitivity and Specificity; Temperature; Time Factors; Yersinia pestis | 2013 |
Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity.
Topics: Blotting, Northern; Digoxigenin; DNA Probes; Humans; Luminescent Measurements; MicroRNAs; Nucleic Acid Hybridization; Sensitivity and Specificity | 2014 |
Generation of digoxigenin-incorporated probes to enhance DNA detection sensitivity.
Topics: Blotting, Southern; Digoxigenin; DNA; Molecular Probe Techniques; Oligonucleotide Probes; Reproducibility of Results; Sensitivity and Specificity; Staining and Labeling; Telomere Homeostasis | 2016 |
Dual-allele dipstick assay for genotyping single nucleotide polymorphisms by primer extension reaction.
Topics: Antibodies, Immobilized; Aryl Hydrocarbon Hydroxylases; Biotin; Cytochrome P-450 CYP2C19; Digoxigenin; DNA Mutational Analysis; DNA Primers; Genotype; Humans; Oligonucleotides; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Reagent Strips; Sensitivity and Specificity; Toll-Like Receptor 4 | 2009 |
Evaluation of a digoxigenin-labelled probe for detection of Aeromonas spp.
Topics: Aeromonas; Animals; Bacterial Typing Techniques; Digoxigenin; DNA Probes; DNA, Bacterial; Humans; Sensitivity and Specificity; Vibrio Infections | 2009 |
Optimization of a digoxigenin-based immunoassay system for gene detection in Arabidopsis thaliana.
Topics: Arabidopsis; Digoxigenin; DNA Probes; DNA, Plant; Genes, Plant; Genome, Plant; Immunoassay; In Situ Hybridization; Peptide Elongation Factor 1; Plasmids; Sensitivity and Specificity | 2009 |
In vivo labeling and specific magnetic bead separation of RNA for biofilm characterization and stress-induced gene expression analysis in bacteria.
Topics: Bacteria; Biofilms; Deoxyuracil Nucleotides; Digoxigenin; Enterococcus faecium; Gene Expression Profiling; Heat-Shock Proteins; Magnetics; Microspheres; Pseudomonas aeruginosa; RNA, Bacterial; RNA, Messenger; Sensitivity and Specificity; Staining and Labeling | 2009 |
A novel homogeneous Biotin-digoxigenin based assay for the detection of human anti-therapeutic antibodies in autoimmune serum.
Topics: Anti-Inflammatory Agents, Non-Steroidal; Antibodies, Anti-Idiotypic; Antibodies, Monoclonal; Arthritis, Rheumatoid; Biotin; Clinical Trials as Topic; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Female; Humans; Luminescent Measurements; Male; Sensitivity and Specificity | 2010 |
A novel strategy for screening-out raw milk contaminated with Mycobacterium bovis on dairy farms by double-tagging PCR and electrochemical genosensing.
Topics: Animals; Bacteriological Techniques; Biotin; Digoxigenin; DNA Primers; DNA Transposable Elements; DNA, Bacterial; Electrochemical Techniques; Mass Screening; Milk; Mycobacterium bovis; Polymerase Chain Reaction; Sensitivity and Specificity; Staining and Labeling | 2010 |
Clinical immunogenicity specificity assessments: a platform evaluation.
Topics: Antibodies; Antibodies, Monoclonal; Antibody Formation; Biotin; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Humans; Luminescent Measurements; Ruthenium; Sensitivity and Specificity | 2011 |
Nonradioactive method to detect native single-stranded G-tails on yeast telomeres using a modified Southern blot protocol.
Topics: Base Sequence; Blotting, Southern; Digoxigenin; DNA, Fungal; DNA, Single-Stranded; Nucleic Acid Hybridization; Oligonucleotide Probes; Saccharomyces cerevisiae; Sensitivity and Specificity; Telomere | 2011 |
Detection of Vibrio parahaemolyticus in shellfish using polymerase chain reaction-enzyme-linked immunosorbent assay.
Topics: Animals; Digoxigenin; DNA, Bacterial; Enzyme-Linked Immunosorbent Assay; Food Contamination; Food Microbiology; Limit of Detection; Oligonucleotide Probes; Polymerase Chain Reaction; Seafood; Sensitivity and Specificity; Shellfish; Vibrio parahaemolyticus | 2012 |
Immobilisation of quantum dots by bio-orthogonal PCR amplification and labelling for direct gene detection and quantitation.
Topics: Antibodies; Biological Assay; Biotin; Digoxigenin; DNA; DNA Primers; Histidine Decarboxylase; Humans; Leukocytes; Plasmids; Point-of-Care Systems; Polymerase Chain Reaction; Quantum Dots; Sensitivity and Specificity; Spectrometry, Fluorescence; Staining and Labeling; Streptavidin | 2012 |
Development and evaluation of a dot blot assay for rapid determination of invasion-associated gene ibeA directly in fresh bacteria cultures of E. coli.
Topics: Animals; Bacteriological Techniques; Carrier State; Chickens; Digoxigenin; Escherichia coli; Escherichia coli Infections; Escherichia coli Proteins; Humans; Membrane Proteins; Nucleic Acid Hybridization; Oligonucleotide Probes; Sensitivity and Specificity; Staining and Labeling; Time Factors; Virulence Factors | 2012 |
Checkerboard DNA-DNA hybridisation technology focused on the analysis of Gram-positive cariogenic bacteria.
Topics: Buffers; Candida albicans; Child; Child, Preschool; Dental Caries; Dental Plaque; Digoxigenin; DNA Probes; DNA, Bacterial; DNA, Fungal; Gram-Positive Bacteria; Humans; Nucleic Acid Hybridization; Sensitivity and Specificity | 2002 |
Development of nonradioactive microtiter plate assays for nuclease activity.
Topics: Aurintricarboxylic Acid; Base Sequence; Biotin; Calibration; Deoxyribonuclease I; Digoxigenin; DNA; Edetic Acid; Enzyme-Linked Immunosorbent Assay; Microspheres; Oligonucleotides; Reference Standards; Reproducibility of Results; Sensitivity and Specificity; Single-Strand Specific DNA and RNA Endonucleases; Streptavidin; Time Factors | 2002 |
In situ hybridization in neural tissues.
Topics: Animals; Biotin; Central Nervous System; Digoxigenin; Fixatives; Humans; Image Processing, Computer-Assisted; In Situ Hybridization; Ribonuclease, Pancreatic; RNA Probes; RNA, Messenger; Sensitivity and Specificity; Staining and Labeling; Temperature; Transcription, Genetic | 2003 |
Non-radioactive hybridization in microwells using enzyme linked immune sorbent assay for detection of RT-PCR-amplified CK19- and CEA-mRNA.
Topics: Bone Marrow Cells; Breast Neoplasms; Carcinoembryonic Antigen; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Female; Humans; Keratins; Neoplastic Cells, Circulating; Nucleic Acid Hybridization; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sensitivity and Specificity | 1999 |
Loss of the expression of the tumor suppressor gene ARHI is associated with progression of breast cancer.
Topics: Adult; Aged; Antibodies, Monoclonal; Blotting, Western; Breast; Breast Neoplasms; Carcinoma, Intraductal, Noninfiltrating; Cyclin D1; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; Cytoplasm; Digoxigenin; Disease Progression; DNA, Complementary; Down-Regulation; Female; Genes, Tumor Suppressor; Humans; Immunohistochemistry; In Situ Hybridization; Middle Aged; Reverse Transcriptase Polymerase Chain Reaction; rho GTP-Binding Proteins; RNA; RNA, Messenger; Sensitivity and Specificity; Transfection | 2003 |
Optimization of in situ hybridization assay using non-radioactive DNA probes for the detection of canine herpesvirus (CHV) in paraffin-embedded sections.
Topics: Animals; Biotin; Cell Line; Digoxigenin; DNA Probes; DNA, Viral; Dog Diseases; Dogs; Herpesviridae Infections; Herpesvirus 1, Canid; In Situ Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity | 2004 |
Two-color, rolling-circle amplification on antibody microarrays for sensitive, multiplexed serum-protein measurements.
Topics: Antibodies; Biotin; Blood Proteins; Color; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Humans; Immunoassay; Liver Cirrhosis; Liver Neoplasms; Protein Array Analysis; Reproducibility of Results; Sensitivity and Specificity | 2004 |
Cloth-based hybridization array system for the detection of multiple antibiotic resistance genes in Salmonella enterica subsp. enterica serotype Typhimurium DT104.
Topics: Bacterial Proteins; beta-Lactam Resistance; Chloramphenicol Resistance; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Bacterial; Drug Resistance, Multiple, Bacterial; Food Microbiology; Genes, Bacterial; Immunoenzyme Techniques; Integrases; Nucleic Acid Hybridization; Polymerase Chain Reaction; Salmonella typhimurium; Sensitivity and Specificity; Tetracycline Resistance; Virulence Factors | 2004 |
Establishment and assessment of two methods for quantitative detection of serum duck hepatitis B virus DNA.
Topics: Alkaline Phosphatase; Animals; Digoxigenin; DNA Probes; DNA, Viral; Ducks; Hepadnaviridae Infections; Hepatitis B Virus, Duck; Hepatitis, Viral, Animal; Polymerase Chain Reaction; Sensitivity and Specificity | 2004 |
Development and evaluation of a new ELISA for the detection and quantification of antierythropoietin antibodies in human sera.
Topics: Animals; Antibodies; Biotin; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Erythropoietin; Humans; Lupus Erythematosus, Systemic; Peroxidase; Rabbits; Recombinant Proteins; Red-Cell Aplasia, Pure; Reference Standards; Reference Values; Renal Dialysis; Reproducibility of Results; Sensitivity and Specificity; Sjogren's Syndrome; Streptavidin | 2004 |
Sensitivity of a digoxigenin-labelled DNA probe in detecting Mikrocytos mackini, causative agent of Denman Island disease (mikrocytosis), in oysters.
Topics: Animals; Digoxigenin; DNA Probes; Eukaryota; In Situ Hybridization; Ostreidae; Protozoan Infections, Animal; Sensitivity and Specificity | 2005 |
Simultaneous identification of orthopoxviruses and alphaviruses by oligonucleotide macroarray with special emphasis on detection of variola and Venezuelan equine encephalitis viruses.
Topics: Alphavirus; Digoxigenin; DNA, Viral; Encephalitis Virus, Venezuelan Equine; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Orthopoxvirus; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Staining and Labeling; Variola virus | 2006 |
Direct identification of Pseudomonas aeruginosa from blood culture bottles by PCR-enzyme linked immunosorbent assay using oprI gene specific primers.
Topics: Bacterial Proteins; Digoxigenin; DNA, Bacterial; Enzyme-Linked Immunosorbent Assay; Humans; Lipoproteins; Oligonucleotide Probes; Polymerase Chain Reaction; Pseudomonas aeruginosa; Sensitivity and Specificity | 2005 |
Detection of type A, B, E, and F Clostridium botulinum neurotoxins in foods by using an amplified enzyme-linked immunosorbent assay with digoxigenin-labeled antibodies.
Topics: Antibodies, Bacterial; Botulinum Toxins; Clostridium botulinum; Cross Reactions; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Food Contamination; Food Microbiology; Neurotoxins; Reference Standards; Sensitivity and Specificity; Serotyping | 2006 |
A high-resolution, double-labeling method for the study of in vivo red blood cell aging.
Topics: Animals; Biotinylation; Digoxigenin; Erythrocyte Aging; Erythrocytes; Female; Flow Cytometry; Rats; Rats, Inbred F344; Sensitivity and Specificity | 2006 |
Development and evaluation of a colorimetric membrane-array method for the detection of circulating tumor cells in the peripheral blood of Taiwanese patients with colorectal cancer.
Topics: Adult; Aged; Aged, 80 and over; Colorectal Neoplasms; Digoxigenin; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Linear Models; Male; Middle Aged; Neoplastic Cells, Circulating; Oligonucleotide Array Sequence Analysis; Reproducibility of Results; Sensitivity and Specificity; Taiwan | 2006 |
Enzyme-linked immunosorbent assay of nucleic acid sequence-based amplification for molecular detection of M. tuberculosis.
Topics: Base Sequence; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Humans; Mycobacterium tuberculosis; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; RNA, Bacterial; RNA, Ribosomal, 16S; Sensitivity and Specificity; Tuberculosis, Pulmonary | 2006 |
Production of anti-digoxigenin antibody HRP conjugate for PCR-ELISA DIG detection system.
Topics: Animals; Antibodies; Antibodies, Monoclonal; Antibody Specificity; Antigen-Antibody Complex; Antigen-Antibody Reactions; Digoxigenin; DNA; Enzyme-Linked Immunosorbent Assay; Horseradish Peroxidase; Male; Polymerase Chain Reaction; Quality Control; Rabbits; Reagent Kits, Diagnostic; Sensitivity and Specificity; Serum Albumin, Bovine | 2006 |
Development and validation of a non-radioactive DNA polymerase assay for studying cytomegalovirus resistance to foscarnet.
Topics: Antiviral Agents; Catalytic Domain; Colorimetry; Cytomegalovirus; Digoxigenin; DNA-Directed DNA Polymerase; Drug Resistance, Viral; Foscarnet; Inhibitory Concentration 50; Mutation; Reproducibility of Results; Sensitivity and Specificity; Viral Proteins | 2007 |
In situ hybridization for Lawsonia intracellularis--specific 16s rRNA sequence in paraffin-embedded tissue using a digoxigenin-labeled oligonucleotide probe.
Topics: Animals; Desulfovibrionaceae Infections; Digoxigenin; DNA, Bacterial; In Situ Hybridization; Intestinal Diseases; Lawsonia Bacteria; Oligonucleotide Probes; Paraffin Embedding; RNA, Ribosomal, 16S; Sensitivity and Specificity; Swine; Swine Diseases | 2007 |
Three digoxigenin-labeled cDNA probes for specific detection of the natural population of Barley yellow dwarf viruses in China by dot-blot hybridization.
Topics: Blotting, Northern; China; Digoxigenin; DNA, Complementary; Luteovirus; Nucleic Acid Hybridization; RNA, Viral; Sensitivity and Specificity; Triticum | 2007 |
Development of a non-radioactive digoxigenin cDNA probe for the detection of avian encephalomyelitis virus.
Topics: Animals; Base Sequence; Chick Embryo; Chickens; Digoxigenin; DNA Probes; DNA, Complementary; Encephalomyelitis Virus, Avian; Picornaviridae Infections; Poultry Diseases; Sensitivity and Specificity | 2008 |
Case report: in situ hybridization for detection of inapparent infection with Chlamydia trachomatis in synovial tissue of a patient with Reiter's syndrome.
Topics: Adult; Aged; Antibodies, Monoclonal; Arthritis, Reactive; Chlamydia Infections; Chlamydia trachomatis; Digoxigenin; DNA Probes; Female; Fluorescent Antibody Technique, Direct; HeLa Cells; Humans; In Situ Hybridization; Male; Osteoarthritis; RNA, Bacterial; RNA, Ribosomal, 16S; Sensitivity and Specificity; Synovial Membrane | 1995 |
Quantitation of digoxigenin-labeled DNA hybridized to DNA and RNA slot blots.
Topics: Animals; Cloning, Molecular; Digoxigenin; DNA; DNA, Complementary; Immunoblotting; Indicators and Reagents; Liver; Lung; Multigene Family; Nucleic Acid Hybridization; Oligonucleotide Probes; Oxygenases; Rabbits; Restriction Mapping; RNA; Sensitivity and Specificity | 1995 |
Application of DNA fingerprinting with digoxigenated oligonucleotide probe (CAC)5 to analysis of the genetic variation within Taenia taeniaeformis.
Topics: Animals; Arvicolinae; Base Sequence; Digoxigenin; DNA Fingerprinting; DNA, Helminth; Electrophoresis, Agar Gel; Genetic Variation; Mice; Molecular Sequence Data; Oligonucleotide Probes; Rats; Restriction Mapping; Sensitivity and Specificity; Taenia | 1995 |
Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids. Part Two: Colorigenic methods and comparison with chemiluminescent methods.
Topics: Adenocarcinoma; Animals; Blotting, Southern; Collodion; Colorimetry; Digoxigenin; DNA; DNA, Neoplasm; Gene Amplification; Luminescent Measurements; Mammary Neoplasms, Experimental; Membranes, Artificial; Mice; Sensitivity and Specificity | 1995 |
Evaluation of non-radioactive labelling and detection of deoxyribonucleic acids. Part One: Chemiluminescent methods.
Topics: Adamantane; Base Sequence; Biotin; Blotting, Southern; Deoxyuracil Nucleotides; Digoxigenin; DNA; Humans; Indicators and Reagents; Luminescent Measurements; Molecular Sequence Data; Peroxidases; Sensitivity and Specificity | 1995 |
Poly A-linked colorimetric microtiter plate assay for HIV reverse transcriptase.
Topics: Biotin; Colorimetry; Deoxyuracil Nucleotides; Digoxigenin; Enzyme-Linked Immunosorbent Assay; HIV Reverse Transcriptase; HIV-1; Humans; Indicators and Reagents; Leukocytes, Mononuclear; Poly A; Retroviridae; RNA-Directed DNA Polymerase; Sensitivity and Specificity | 1993 |
Chemiluminescent and colorigenic detection of cherry leaf roll virus with digoxigenin-labeled RNA probes.
Topics: Blotting, Northern; Digoxigenin; Fruit; Immunoblotting; Luminescent Measurements; Nepovirus; RNA Probes; RNA, Viral; Sensitivity and Specificity; Staining and Labeling | 1993 |
An integrated target sequence and signal amplification assay, reverse transcriptase-PCR-enzyme-linked immunosorbent assay, to detect and characterize flaviviruses.
Topics: Aedes; Animals; Base Sequence; Biotin; Culex; Dengue; Dengue Virus; Digoxigenin; DNA Probes; DNA, Viral; Encephalitis Virus, St. Louis; Enzyme-Linked Immunosorbent Assay; Flavivirus; Gene Amplification; Humans; Molecular Sequence Data; Polymerase Chain Reaction; RNA-Directed DNA Polymerase; RNA, Viral; Sensitivity and Specificity; Viremia | 1994 |
A modified nonradioactive method for northern blot analysis.
Topics: Base Sequence; Blotting, Northern; Digoxigenin; Humans; Luminescent Measurements; Molecular Sequence Data; Polymerase Chain Reaction; RNA; RNA Probes; RNA, Messenger; Sensitivity and Specificity | 1994 |
Nonradioactive labeling and high-sensitive detection of PCR products.
Topics: Biotechnology; Biotin; Blotting, Southern; Digoxigenin; DNA; DNA-Directed DNA Polymerase; Electrophoresis, Agar Gel; Gene Amplification; Immunoassay; Molecular Probes; Nucleic Acid Hybridization; Polymerase Chain Reaction; RNA; Sensitivity and Specificity; Taq Polymerase | 1994 |
A comparison of digoxigenin and biotin labelled DNA and RNA probes for in situ hybridization.
Topics: Animals; Biotin; Brain; Chickens; Digoxigenin; DNA, Viral; Humans; In Situ Hybridization; Measles virus; Molecular Probe Techniques; RNA, Viral; Sensitivity and Specificity | 1995 |
Characterization of a cloned pR72H probe for Vibrio parahaemolyticus detection and development of a nonisotopic colony hybridization assay.
Topics: Chromosomes, Bacterial; Cloning, Molecular; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Bacterial; Food Microbiology; Foodborne Diseases; Indicators and Reagents; Nucleic Acid Hybridization; Plasmids; Sensitivity and Specificity; Vibrio Infections; Vibrio parahaemolyticus | 1995 |
Synthetic oligonucleotide cocktails as probes for detection of human parvovirus B19.
Topics: Base Sequence; Digoxigenin; Dinitrobenzenes; DNA, Viral; Humans; Hydrops Fetalis; In Situ Hybridization; Molecular Sequence Data; Oligonucleotide Probes; Parvovirus B19, Human; Sensitivity and Specificity | 1995 |
D1 and D2 dopamine receptor gene expression in the rat striatum: sensitive cRNA probes demonstrate prominent segregation of D1 and D2 mRNAs in distinct neuronal populations of the dorsal and ventral striatum.
Topics: Animals; Corpus Striatum; Digoxigenin; Efferent Pathways; Gene Expression Regulation; In Situ Hybridization; Neurons; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D1; Receptors, Dopamine D2; Reference Values; RNA Probes; RNA, Complementary; RNA, Messenger; Sensitivity and Specificity | 1995 |
Adaptation of a non-radioactive in situ hybridization method to electron microscopy: detection of tenascin mRNAs in mouse cerebellum with digoxigenin-labelled probes and gold-labelled antibodies.
Topics: Acrylic Resins; Animals; Astrocytes; Cell Adhesion Molecules, Neuronal; Cerebellum; Digoxigenin; Extracellular Matrix Proteins; Immunohistochemistry; In Situ Hybridization; Mice; Mice, Inbred ICR; Microscopy, Electron; Microtomy; Purkinje Cells; RNA Probes; RNA, Messenger; Sensitivity and Specificity; Sulfur Radioisotopes; Tenascin; Tissue Embedding; Transcription, Genetic | 1993 |
Reduction of background problems in nonradioactive northern and Southern blot analyses enables higher sensitivity than 32P-based hybridizations.
Topics: Alkaline Phosphatase; Animals; Blotting, Northern; Blotting, Southern; Digoxigenin; DNA; Isotope Labeling; Luminescent Measurements; Mice; Nucleic Acid Hybridization; Phosphorus Radioisotopes; RNA; RNA, Messenger; Sensitivity and Specificity | 1993 |
Chemiluminescent enzyme-linked immunoassay for reverse transcriptase, illustrated by detection of HIV reverse transcriptase.
Topics: Alkaline Phosphatase; Antibodies; Bacterial Proteins; Biotin; Deoxyuracil Nucleotides; Digoxigenin; Enzyme-Linked Immunosorbent Assay; HIV Infections; HIV Reverse Transcriptase; Humans; Luminescent Measurements; Lymphocytes; Oligodeoxyribonucleotides; Poly A; Reference Standards; RNA-Directed DNA Polymerase; Sensitivity and Specificity; Streptavidin; Time Factors | 1993 |
Spectrophotometric quantitation of DNA on blots after ethanol-solubilization of the MTT-formazan from anti-digoxigenin-based detection of nucleic acids.
Topics: Antibodies; Blotting, Southern; Digoxigenin; DNA; Ethanol; Formazans; Sensitivity and Specificity; Solubility; Spectrophotometry; Staining and Labeling; Tetrazolium Salts | 1993 |
Resin-purified digoxigenin-labeled DNA probes: an efficient protocol for mapping and fingerprinting sugarcane.
Topics: Anion Exchange Resins; Artifacts; Chromatography, Ion Exchange; Chromosome Mapping; Digoxigenin; DNA Fingerprinting; DNA Probes; DNA, Plant; Sensitivity and Specificity | 1995 |
In situ hybridization with digoxigenin-labeled RNA probes: facts and artifacts.
Topics: Alkaline Phosphatase; Animals; Artifacts; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Frozen Sections; In Situ Hybridization; Interferon-gamma; Interleukin-2; Male; Mice; Mice, Inbred C57BL; RNA Probes; Sensitivity and Specificity | 1995 |
Digoxigenin-labelled cDNA probes for the detection of potato virus Y in dormant potato tubers.
Topics: Digoxigenin; DNA Probes; DNA, Complementary; DNA, Viral; Indicators and Reagents; Potyvirus; Sensitivity and Specificity; Solanum tuberosum | 1995 |
In situ hybridization for cytokine mRNA with digoxigenin-labeled riboprobes. Sensitivity of detection and double label applications.
Topics: Animals; Cytokines; Digoxigenin; Female; In Situ Hybridization; Mice; Mice, Inbred BALB C; Mice, Transgenic; Receptors, Antigen, T-Cell, alpha-beta; RNA Probes; RNA, Messenger; Sensitivity and Specificity | 1995 |
Microtitration plate enzyme immunoassay to detect PCR-amplified DNA from Candida species in blood.
Topics: Animals; Base Sequence; Candida; Candidiasis; Digoxigenin; DNA Primers; DNA, Fungal; DNA, Ribosomal; Electrophoresis, Agar Gel; Ethidium; Evaluation Studies as Topic; Humans; Immunoenzyme Techniques; Molecular Probe Techniques; Molecular Sequence Data; Mycology; Oligonucleotide Probes; Polymerase Chain Reaction; Rabbits; Sensitivity and Specificity | 1995 |
Co-expression of collagen types II and X mRNAs in newly formed hypertrophic chondrocytes of the embryonic chick vertebral body demonstrated by double-fluorescence in situ hybridization.
Topics: Animals; Biotin; Cartilage; Chick Embryo; Collagen; Digoxigenin; DNA Probes; DNA, Complementary; Gene Expression Regulation, Developmental; In Situ Hybridization, Fluorescence; Osteogenesis; RNA, Messenger; Sensitivity and Specificity; Thoracic Vertebrae; Thymine Nucleotides; Tritium | 1994 |
Digoxin immunoassay with cross-reactivity of digoxin metabolites proportional to their biological activity.
Topics: Animals; Biological Assay; Cats; Digoxigenin; Digoxin; Guinea Pigs; Heart; Humans; Immunoassay; Mice; Ouabain; Sensitivity and Specificity; Sodium-Potassium-Exchanging ATPase | 1994 |
Rapid diagnosis of cytomegalovirus encephalitis in patients with AIDS using in situ hybridisation.
Topics: Adult; AIDS-Related Opportunistic Infections; Cytomegalovirus; Cytomegalovirus Infections; Digoxigenin; DNA Probes; DNA, Viral; Encephalitis, Viral; Female; Fluorescent Antibody Technique; Humans; In Situ Hybridization; Male; Middle Aged; Sensitivity and Specificity | 1994 |
Detection of herpes simplex virus DNA from cerebrospinal fluid by PCR and a rapid, nonradioactive hybridization technique.
Topics: Artifacts; Base Sequence; Blotting, Southern; Digoxigenin; DNA Primers; DNA-Directed DNA Polymerase; DNA, Viral; Herpes Simplex; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity | 1994 |
A sensitive method of non-radioactive in situ hybridization for mRNA localization within human renal biopsy specimens: use of digoxigenin labeled oligonucleotides.
Topics: Actins; Base Sequence; Biopsy; Digoxigenin; Glomerulonephritis, IGA; Humans; Immunoenzyme Techniques; In Situ Hybridization; Interleukin-6; Kidney; Molecular Sequence Data; Oligonucleotide Probes; RNA, Messenger; Sensitivity and Specificity | 1994 |
Labeling of basic fibroblast growth factor with digoxigenin: a nonradioactive probe for biochemical and cytological applications.
Topics: Animals; Aorta, Thoracic; Blotting, Western; Cattle; Cell Division; Cell Line; Cells, Cultured; Cricetinae; Digoxigenin; Endocytosis; Endothelium, Vascular; Enzyme-Linked Immunosorbent Assay; Fibroblast Growth Factor 2; Fluorescent Antibody Technique; Humans; Indicators and Reagents; Kinetics; Microscopy, Electron; Receptors, Fibroblast Growth Factor; Recombinant Proteins; Sensitivity and Specificity | 1994 |
Synthesis of a non-radioactive hepatitis B virus DNA probe from human serum by the polymerase chain reaction.
Topics: Base Sequence; Deoxyuracil Nucleotides; Digoxigenin; DNA Primers; DNA Probes; DNA, Viral; Electrophoresis, Agar Gel; Hepatitis B; Hepatitis B Antibodies; Hepatitis B e Antigens; Hepatitis B virus; Humans; Indicators and Reagents; Molecular Sequence Data; Polymerase Chain Reaction; Sensitivity and Specificity | 1994 |
Evaluation of strand-specific RNA probes visualized by colorimetric and chemiluminescent reactions for the detection of B19 parvovirus DNA.
Topics: Adult; Blotting, Southern; Child; Colorimetry; Digoxigenin; DNA, Viral; Evaluation Studies as Topic; Female; Humans; Luminescent Measurements; Male; Molecular Probe Techniques; Nucleic Acid Hybridization; Parvovirus B19, Human; Pregnancy; RNA Probes; Sensitivity and Specificity; Virology | 1993 |
Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer.
Topics: Animals; Base Sequence; Cloning, Molecular; Digoxigenin; DNA Fingerprinting; DNA Probes; DNA, Protozoan; DNA, Ribosomal; Feces; Insect Vectors; Luminescent Measurements; Molecular Sequence Data; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Rhodnius; Sensitivity and Specificity; Species Specificity; Trypanosoma cruzi | 1994 |
Rapid detection of PCR products of hepatitis C virus (HCV) with a non-radioactive oligoprobe.
Topics: Base Sequence; Blotting, Southern; Chronic Disease; Digoxigenin; Hepacivirus; Hepatitis C; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity | 1994 |
Microplate capture hybridization of amplified parvovirus B19 DNA fragment labelled with digoxigenin.
Topics: Base Sequence; Blotting, Southern; Digoxigenin; DNA Primers; DNA, Viral; Genes, Viral; Humans; Immunocompromised Host; Molecular Sequence Data; Nucleic Acid Hybridization; Parvovirus B19, Human; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Viral Structural Proteins | 1993 |
In situ polymerase chain reaction: localization of HSV-2 DNA sequences in infections of the nervous system.
Topics: Acute Disease; Animals; Base Sequence; Brain; Digoxigenin; DNA, Viral; Herpes Simplex; In Situ Hybridization; Mice; Molecular Sequence Data; Polymerase Chain Reaction; Sensitivity and Specificity; Simplexvirus; Trigeminal Ganglion; Virus Latency | 1994 |
A polymerase chain reaction-sequence-specific oligonucleotide procedure for HLA class II typing using biotin- and digoxigenin-labeled probes simultaneously in hybridization.
Topics: Base Sequence; Biotin; Cell Line; Digoxigenin; DNA; Genotype; HLA-DR Antigens; Humans; Immunoblotting; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1994 |
Digoxigenin-ampicillin conjugate for detection of penicillin-binding proteins by chemiluminescence.
Topics: Ampicillin; Bacterial Proteins; Carrier Proteins; Chromatography, High Pressure Liquid; Digoxigenin; Escherichia coli; Hexosyltransferases; Immunoblotting; Luminescent Measurements; Muramoylpentapeptide Carboxypeptidase; Penicillin-Binding Proteins; Peptidyl Transferases; Sensitivity and Specificity | 1994 |
Detection of HIV-1 by digoxigenin-labelled PCR and microtitre plate solution hybridisation assay and prevention of PCR carry-over by uracil-N-glycosylase.
Topics: Base Sequence; Deoxyuracil Nucleotides; Digoxigenin; DNA Glycosylases; DNA Primers; DNA Probes; DNA, Antisense; DNA, Viral; Genes, env; Genes, gag; HIV-1; Microchemistry; Molecular Sequence Data; N-Glycosyl Hydrolases; Nucleic Acid Hybridization; Polymerase Chain Reaction; Proviruses; Sensitivity and Specificity; Uracil-DNA Glycosidase | 1993 |
Quantitative measurement of nonisotopically labeled polymerase chain reaction product.
Topics: Base Sequence; Biotin; Digoxigenin; DNA; DNA Probes; Isotope Labeling; Molecular Sequence Data; Polymerase Chain Reaction; RNA Probes; Sensitivity and Specificity | 1993 |
In situ hybridization of virulent canine distemper virus in brain tissue, using digoxigenin-labeled probes.
Topics: Animals; Brain; Cells, Cultured; Digoxigenin; Distemper; Distemper Virus, Canine; DNA, Complementary; Dogs; In Situ Hybridization; RNA, Viral; Sensitivity and Specificity | 1993 |
Sensitive and quantitative detection of PCR-amplified HIV-1 DNA products by an enzyme linked immunoassay following solution hybridization with two differently labelled oligonucleotide probes.
Topics: Base Sequence; Biotin; Cell Line; Digoxigenin; DNA Primers; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Genes, gag; HIV-1; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1993 |
Detection of low copy numbers of Epstein-Barr virus by in situ hybridization using nonradioisotopic probes prepared by the polymerase chain reaction.
Topics: Base Sequence; Cell Line; Dactinomycin; Deoxyribonucleases; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; Herpesvirus 4, Human; Humans; In Situ Hybridization; Molecular Sequence Data; Polymerase Chain Reaction; Ribonucleases; Sensitivity and Specificity | 1993 |
Fine-structural localization of proenkephalin mRNAs in the hypothalamic magnocellular dorsal nucleus of the guinea pig: a comparison of radioisotopic and enzymatic in situ hybridization methods at the light- and electron-microscopic levels.
Topics: Alkaline Phosphatase; Animals; Biotin; Cytoplasm; Digoxigenin; Endoplasmic Reticulum; Enkephalins; Guinea Pigs; Hypothalamus; In Situ Hybridization; Male; Microscopy, Electron; Nuclear Envelope; Oligonucleotide Probes; Protein Precursors; RNA, Messenger; Sensitivity and Specificity; Sulfur Radioisotopes | 1993 |
A rapid method for quantitation of oligodeoxynucleotide phosphorothioates in biological fluids and tissues.
Topics: Animals; Base Sequence; Chemistry Techniques, Analytical; Deoxyuracil Nucleotides; Dideoxynucleotides; Digoxigenin; Indicators and Reagents; Kidney; Male; Mice; Mice, Inbred Strains; Molecular Sequence Data; Oligonucleotide Probes; Oligonucleotides; Sensitivity and Specificity; Thionucleotides | 1993 |
The development and assessment of DNA and oligonucleotide probes for the specific detection of Bacillus anthracis.
Topics: Bacillus anthracis; Base Sequence; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Bacterial; Environmental Microbiology; Indicators and Reagents; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Sensitivity and Specificity; Virulence | 1993 |
Evaluation of a technique for identification of Shiga-like toxin-producing Escherichia coli by using polymerase chain reaction and digoxigenin-labeled probes.
Topics: Antibodies, Monoclonal; Bacterial Toxins; Base Sequence; Digoxigenin; DNA Primers; DNA Probes; DNA, Bacterial; Escherichia coli; Escherichia coli Infections; Evaluation Studies as Topic; Genes, Bacterial; Humans; Molecular Sequence Data; Neutralization Tests; Polymerase Chain Reaction; Sensitivity and Specificity; Shiga Toxin 1; Shiga Toxin 2; Shigella dysenteriae | 1993 |
Polymerase chain reaction for synthesis of digoxigenin-labelled DNA probe: application to parvovirus B19 and to polyomavirus BK.
Topics: Base Sequence; BK Virus; Digoxigenin; DNA Probes; Humans; Molecular Sequence Data; Parvoviridae Infections; Parvovirus B19, Human; Polymerase Chain Reaction; Sensitivity and Specificity; Templates, Genetic; Time Factors; Tumor Virus Infections | 1993 |
Chemiluminescent detection of infectious bursal disease virus with a PCR-generated nonradiolabeled probe.
Topics: Animals; Base Sequence; Chickens; Digoxigenin; Infectious bursal disease virus; Luminescent Measurements; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1993 |
The release of soluble p55 TNF receptor from U937 cells studied by a new p55 immunoassay.
Topics: Animals; Antibodies, Monoclonal; Antibody Specificity; Antiviral Agents; Blotting, Northern; Brefeldin A; Cell Line; Cycloheximide; Cyclopentanes; Digoxigenin; Dose-Response Relationship, Immunologic; Flow Cytometry; Humans; Hybridomas; Immunoassay; Mice; Mice, Inbred BALB C; Monensin; Monocytes; Precipitin Tests; Receptors, Cell Surface; Receptors, Tumor Necrosis Factor; RNA, Messenger; Sensitivity and Specificity; Tetradecanoylphorbol Acetate; Tumor Necrosis Factor-alpha | 1993 |
Detection of Mycobacterium tuberculosis in clinical samples by two-step polymerase chain reaction and nonisotopic hybridization methods.
Topics: Alkaline Phosphatase; Base Sequence; Blotting, Southern; Colony Count, Microbial; Digoxigenin; DNA, Bacterial; Electrophoresis, Agar Gel; False Positive Reactions; Humans; Molecular Sequence Data; Mycobacterium tuberculosis; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity; Sputum | 1993 |
In situ detection of human Ig light-chain mRNA on formalin-fixed and paraffin-embedded tissue sections using digoxigenin-labelled RNA probes.
Topics: Antisense Elements (Genetics); Digoxigenin; Humans; Immunoglobulin Light Chains; In Situ Hybridization; Lymphoma, B-Cell; Palatine Tonsil; Plasma Cells; RNA Probes; RNA, Messenger; Sensitivity and Specificity; Temperature; Transcription, Genetic | 1993 |
Digoxigenin-labelled inv- and ail-probes for the detection and identification of pathogenic Yersinia enterocolitica in clinical specimens and naturally contaminated pig samples.
Topics: Abattoirs; Animals; Bacterial Outer Membrane Proteins; Digoxigenin; Feces; Genes, Bacterial; Humans; Immunoblotting; Nucleic Acid Hybridization; Plasmids; Sensitivity and Specificity; Serotyping; Swine; Swine Diseases; Yersinia enterocolitica; Yersinia Infections | 1993 |
A highly sensitive and fast nonradioactive method for detection of polymerase chain reaction products.
Topics: Adamantane; Bacterial Proteins; Biotin; Deoxyuracil Nucleotides; Digoxigenin; DNA, Bacterial; Enzymes, Immobilized; Hymecromone; Indicators and Reagents; Listeria monocytogenes; Nitrophenols; Organophosphorus Compounds; Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin; Substrate Specificity; Thymine Nucleotides | 1993 |
Colorimetric detection for PCR amplified HIV-1 DNA using magnetic beads.
Topics: Bacterial Proteins; Base Sequence; Biotin; Colorimetry; Digoxigenin; DNA Probes; DNA, Single-Stranded; DNA, Viral; HIV-1; Humans; Magnetics; Microspheres; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Streptavidin; Time Factors | 1993 |
Quantification of chemiluminescent DNA probes using liquid scintillation counting.
Topics: Adamantane; Alkaline Phosphatase; Bacteriophage lambda; Densitometry; Digoxigenin; DNA Probes; DNA, Viral; Fluorescent Dyes; Immunoblotting; Kinetics; Light; Luminescent Measurements; Membranes, Artificial; Nylons; Scintillation Counting; Sensitivity and Specificity | 1993 |
Stripping of digoxigenin-labeled probes from nylon membranes.
Topics: Digoxigenin; Electrophoresis, Agar Gel; Membranes, Artificial; Nucleic Acid Hybridization; Nucleic Acid Probes; Nylons; Sensitivity and Specificity | 1995 |
Automated detection of digoxigenin-labelled B19 parvovirus amplicons by a capture hybridization assay.
Topics: Bacterial Proteins; Base Sequence; Biotin; Digoxigenin; DNA Probes; DNA, Viral; Erythema Infectiosum; Female; Humans; Immunoenzyme Techniques; Immunoglobulin Fab Fragments; Male; Molecular Sequence Data; Nucleic Acid Hybridization; Parvovirus B19, Human; Reagent Kits, Diagnostic; Reproducibility of Results; Sensitivity and Specificity; Streptavidin | 1995 |
Enzyme-labelled antibody-avidin conjugates: new flexible and sensitive immunochemical reagents.
Topics: Alkaline Phosphatase; Animals; Antigen-Antibody Complex; Avidin; Digoxigenin; Enzyme-Linked Immunosorbent Assay; Goats; Haptens; Horseradish Peroxidase; Immunoblotting; Immunoglobulin G; Indicators and Reagents; Mice; Sensitivity and Specificity | 1996 |
Detection of Anaplasma marginale DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCR-mediated digoxigenin-labeled DNA probe.
Topics: Anaplasma; Anaplasmosis; Animals; Bacteremia; Cattle; Digoxigenin; DNA Probes; DNA, Bacterial; Erythrocytes; In Situ Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Splenectomy; Ticks; Time Factors | 1995 |
The use of digoxigenin-labelled probes to detect DNA sequences specific for plant pathogenic bacteria.
Topics: Bacteria; Blotting, Southern; Digoxigenin; DNA Probes; DNA, Bacterial; DNA, Plant; Evaluation Studies as Topic; Genome, Bacterial; Genome, Plant; Molecular Probe Techniques; Nucleic Acid Hybridization; Plants; Plants, Genetically Modified; Plasmids; Pseudomonas; Rhizobium; Sensitivity and Specificity; Transformation, Genetic | 1995 |
Automated determination of amplified PCR products: application to HCV viremia detection and quantification.
Topics: Base Sequence; Blotting, Southern; Digoxigenin; DNA Primers; DNA Probes; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Gene Amplification; Hepatitis C; Humans; Molecular Probe Techniques; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity; Viremia | 1995 |
Sensitive northern blot hybridization using digoxigenin RNA probes for the mRNA detection of two glucose transporter isoforms.
Topics: Animals; Blotting, Northern; Digoxigenin; Evaluation Studies as Topic; Gene Expression; Glucose Transporter Type 1; Glucose Transporter Type 4; Humans; Molecular Probe Techniques; Monosaccharide Transport Proteins; Muscle Proteins; Phosphorus Radioisotopes; Rats; RNA Probes; RNA, Messenger; Sensitivity and Specificity | 1995 |
Use of digoxigenin-labelled probes for the detection of B19 parvovirus DNA in batches of blood products.
Topics: Blood; Blood Coagulation Factors; Digoxigenin; DNA, Viral; Erythema Infectiosum; Evaluation Studies as Topic; Humans; Molecular Probe Techniques; Molecular Probes; Parvovirus B19, Human; Polymerase Chain Reaction; Sensitivity and Specificity; Serum Albumin; Viremia | 1995 |
Widespread programmed cell death in proliferative and postmitotic regions of the fetal cerebral cortex.
Topics: Animals; Apoptosis; Base Sequence; Cell Division; Cerebral Cortex; Deoxycytosine Nucleotides; Deoxyuracil Nucleotides; Dexamethasone; Digoxigenin; DNA; Embryonic and Fetal Development; Indicators and Reagents; Mice; Mice, Inbred BALB C; Mitosis; Molecular Sequence Data; Neurons; Nucleosomes; Polymerase Chain Reaction; Sensitivity and Specificity; Thymus Gland; Ultraviolet Rays | 1996 |
Comparison of randomly cloned and whole genomic DNA probes for the detection of Porphyromonas gingivalis and Bacteroides forsythus.
Topics: Bacterial Typing Techniques; Bacteroides; Campylobacter; Cloning, Molecular; Colorimetry; Dental Plaque; Digoxigenin; DNA Probes; DNA, Bacterial; Genome, Bacterial; Nucleic Acid Hybridization; Porphyromonas gingivalis; Prevotella; Reproducibility of Results; Sensitivity and Specificity; Species Specificity | 1996 |
An alternative to digoxigenin-labeled primers for manual nonradioactive sequencing allows reading of more than 700 bases.
Topics: Digoxigenin; DNA Primers; Nucleic Acid Hybridization; Sensitivity and Specificity; Sequence Analysis, DNA | 1996 |
Quantification of polymerase chain reaction products: enzyme immunoassay based systems for digoxigenin- and biotin-labelled products that quantify either total or specific amplicons.
Topics: Animals; Base Sequence; Biotin; Cattle; Deoxyuracil Nucleotides; Digoxigenin; DNA; Endothelium; Glyceraldehyde-3-Phosphate Dehydrogenases; Hydrogen-Ion Concentration; Immunoenzyme Techniques; Luminescent Measurements; Molecular Sequence Data; Peptidyl-Dipeptidase A; Polymerase Chain Reaction; Rats; Sensitivity and Specificity; Xanthine Oxidase | 1996 |
Ultra-specific immunoassays for small molecules: roles of wash steps and multiple binding formats.
Topics: Antibodies, Monoclonal; Digitoxigenin; Digitoxin; Digoxigenin; Digoxin; Immunoassay; Sensitivity and Specificity; Strophanthidin | 1996 |
A rapid and sensitive dot-blot hybridization assay for the detection of citrus exocortis viroid in Citrus medica with digoxigenin-labelled RNA probes.
Topics: Digoxigenin; Evaluation Studies as Topic; Formaldehyde; Fruit; Nucleic Acid Hybridization; Plant Viruses; Reproducibility of Results; RNA Probes; RNA, Viral; Sensitivity and Specificity; Viroids | 1996 |
In situ detection of hepatitis C virus RNA in liver tissue using a digoxigenin-labeled probe created during a polymerase chain reaction.
Topics: Adult; Aged; Base Sequence; Coloring Agents; Digoxigenin; DNA Primers; DNA Probes; DNA, Viral; Female; Hepacivirus; Hepatitis C; Humans; In Situ Hybridization; Liver; Male; Middle Aged; Molecular Sequence Data; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity | 1996 |
The photoreactive carbohydrate probe, a novel method for detection of lectins.
Topics: Carbohydrates; Cross-Linking Reagents; Digoxigenin; Glucose; Lectins; Lysine; Photochemistry; Sensitivity and Specificity | 1995 |
Localization of calcitonin mRNA using in situ labeling with reverse transcriptase and PCR.
Topics: Animals; Calcitonin; Digoxigenin; Exons; In Situ Hybridization; Polymerase Chain Reaction; Rats; RNA-Directed DNA Polymerase; RNA, Messenger; Sensitivity and Specificity; Thyroid Gland | 1996 |
Mapping of polyomavirus DNA replicative intermediates by two-dimensional gel analysis using chemiluminescent detection.
Topics: Animals; Cell Line; Digoxigenin; DNA, Viral; Electrophoresis, Gel, Two-Dimensional; Luminescent Measurements; Mice; Polyomavirus; Sensitivity and Specificity | 1996 |
Use of non-radioactive probes for VP4 typing of human rotaviruses.
Topics: Blotting, Northern; Capsid; Capsid Proteins; Cost-Benefit Analysis; Digoxigenin; DNA Probes; Gastroenteritis; Humans; RNA, Viral; Rotavirus; Sensitivity and Specificity | 1996 |
Microwave treatment enhances detection of RNA and DNA by in situ hybridization.
Topics: Digoxigenin; DNA; Humans; In Situ Hybridization; Microwaves; Oligonucleotide Probes; Paraffin Embedding; RNA; RNA, Messenger; Sensitivity and Specificity; Tissue Fixation | 1996 |
Detection of single-copy sequences with digoxigenin-labeled probes in a complex plant genome after separation on pulsed-field gels.
Topics: Blotting, Southern; Digoxigenin; DNA, Plant; Electrophoresis, Gel, Pulsed-Field; Gene Dosage; Genome; Hordeum; Membranes, Artificial; Nucleic Acid Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1996 |
Simple detection of the 5S ribosomal RNA of Pneumocystis carinii using in situ hybridisation.
Topics: Animals; Case-Control Studies; Digoxigenin; DNA Probes; Humans; Immunohistochemistry; In Situ Hybridization; Pneumocystis; Pneumonia, Pneumocystis; Polymerase Chain Reaction; Rats; RNA, Ribosomal, 5S; Sensitivity and Specificity | 1996 |
Sensitive nonradioactive dot blot/ribonuclease protection assay for quantitative determination of mRNA.
Topics: Animals; Blotting, Northern; Densitometry; Digoxigenin; Electrophoresis, Agar Gel; Liver; Nucleic Acid Hybridization; Rats; Ribonuclease, Pancreatic; RNA, Complementary; RNA, Messenger; Sensitivity and Specificity; Temperature; Time Factors | 1997 |
HLA class II typing in a microtitre plate format using digoxigenin-labelled amplified DNA and biotin-labelled oligonucleotide probes.
Topics: Base Sequence; Biotin; Digoxigenin; DNA; DNA Primers; Evaluation Studies as Topic; Histocompatibility Testing; HLA-D Antigens; Humans; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1993 |
Low background and short exposure times in mapping of DNaseI-hypersensitive sites using digoxigenin-labeled probes.
Topics: Animals; Chickens; Chromatin; Chromosome Mapping; Deoxyribonuclease I; Digoxigenin; In Vitro Techniques; Molecular Probe Techniques; RNA Probes; Sensitivity and Specificity | 1997 |
Sensitive chemiluminescence in situ hybridization for the detection of human papillomavirus genomes in biopsy specimens.
Topics: Biotin; Digoxigenin; DNA, Viral; Fluorescein; Fluoresceins; Genome, Viral; HeLa Cells; Humans; In Situ Hybridization; Luminescent Measurements; Papillomaviridae; Papillomavirus Infections; Reproducibility of Results; Sensitivity and Specificity; Tumor Cells, Cultured; Tumor Virus Infections | 1997 |
Quantitative assessment of hepatitis C virus RNA by polymerase chain reaction and a digoxigenin detection system: comparison with branched DNA assay.
Topics: Acute Disease; Adult; Aged; Digoxigenin; DNA; Female; Hepacivirus; Hepatitis C; Humans; Interferons; Male; Middle Aged; Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity | 1997 |
Directional immobilization of heparin onto the nonporous surface of polystyrene microplates.
Topics: Alkaline Phosphatase; Aniline Compounds; Biotin; Chromatography, Affinity; Digoxigenin; Heparin; Heparin Lyase; Indicators and Reagents; Microchemistry; Organophosphorus Compounds; Polystyrenes; Sensitivity and Specificity; Sodium Bicarbonate | 1997 |
[Detection of Mycobacterium tuberculosis in sputum using PCR and chemoluminescent display].
Topics: Bacteriological Techniques; Digoxigenin; DNA, Bacterial; Humans; Luminescent Measurements; Mycobacterium tuberculosis; Polymerase Chain Reaction; Sensitivity and Specificity; Sputum; Tuberculosis, Pulmonary | 1997 |
A porous silicon-based optical interferometric biosensor.
Topics: Animals; Biosensing Techniques; Biotinylation; Digoxigenin; DNA, Complementary; Interferometry; Mice; Sensitivity and Specificity; Silicon; Streptavidin | 1997 |
Evaluation of performances of three DNA enzyme immunoassays for detection of Helicobacter pylori PCR products from biopsy specimens.
Topics: Biopsy; Colorimetry; Digoxigenin; DNA, Bacterial; Enzyme-Linked Immunosorbent Assay; Gastric Mucosa; Genes, Bacterial; Helicobacter Infections; Helicobacter pylori; Humans; Immunoenzyme Techniques; Indicators and Reagents; Nucleic Acid Hybridization; Polymerase Chain Reaction; Reproducibility of Results; Sensitivity and Specificity; Stomach | 1997 |
DNA-probes for the differentiation of Capnocytophaga species.
Topics: Bacterial Typing Techniques; Capnocytophaga; Digoxigenin; DNA Probes; DNA, Bacterial; DNA, Ribosomal; Nucleic Acid Hybridization; Oligodeoxyribonucleotides; RNA, Ribosomal, 16S; Sensitivity and Specificity; Species Specificity | 1997 |
"Checkerboard" versus culture: a comparison between two methods for identification of subgingival microbiota.
Topics: Adult; Aged; Aggregatibacter actinomycetemcomitans; Bacteria; Bacteriological Techniques; Bacteroides; Campylobacter; Colony Count, Microbial; Culture Media; Dental Plaque; Digoxigenin; DNA Probes; DNA, Bacterial; Eikenella corrodens; Fusobacterium nucleatum; Genome, Bacterial; Gingiva; Humans; Middle Aged; Nucleic Acid Hybridization; Periodontal Diseases; Porphyromonas gingivalis; Prevotella; Prevotella intermedia; Sensitivity and Specificity; Streptococcus mutans; Streptococcus sanguis | 1997 |
Design of non-competitive flow injection enzyme immunoassays for determination of haptens: application to digoxigenin.
Topics: Chromatography, Affinity; Digoxigenin; Flow Injection Analysis; Haptens; Immunoenzyme Techniques; Kinetics; Sensitivity and Specificity | 1997 |
Nonradioisotopic telomeric repeat amplification protocol (TRAP) using digoxigenin labeled probe.
Topics: Blotting, Southern; Digoxigenin; Female; Gene Amplification; Humans; Polymerase Chain Reaction; Repetitive Sequences, Nucleic Acid; Sensitivity and Specificity; Telomerase; Tumor Cells, Cultured | 1997 |
An improved protocol to increase sensitivity of Southern blot using dig-labelled DNA probes.
Topics: Animals; Blotting, Southern; Digoxigenin; DNA Probes; Electrophoresis; Luminescent Measurements; Nucleic Acid Hybridization; Rats; Rats, Sprague-Dawley; Sensitivity and Specificity | 1997 |
[HLA-DR typing using PCR-SSO with simultaneous hybridization of each dot blot membrane with a digoxigenin- and a biotin-marked probe].
Topics: Biotin; Digoxigenin; Histocompatibility Testing; HLA-DR Antigens; Humans; In Situ Hybridization; Oligonucleotide Probes; Polymerase Chain Reaction; Sensitivity and Specificity | 1994 |
A PCR-microplate capture hybridization method to detect Listeria monocytogenes in blood.
Topics: Animals; Bacteremia; Blood; Digoxigenin; DNA Probes; Humans; In Situ Hybridization; Listeria monocytogenes; Polymerase Chain Reaction; Rats; Sensitivity and Specificity | 1997 |
Detection of Babesia bovis using a DIG-labeled DNA probe.
Topics: Affinity Labels; Animals; Azides; Babesia bovis; Babesiosis; Biotin; Cattle; Cattle Diseases; Digoxigenin; DNA Probes; DNA, Protozoan; Electrophoresis, Agar Gel; Genetic Techniques; Nucleic Acid Hybridization; Sensitivity and Specificity | 1997 |
An in situ hybridization histochemistry technique allowing simultaneous visualization by the use of confocal microscopy of three cellular mRNA species in individual neurons.
Topics: Adrenal Glands; Animals; Arginine Vasopressin; Biotin; Corticotropin-Releasing Hormone; Digoxigenin; Histological Techniques; In Situ Hybridization; Male; Microscopy, Confocal; Mixed Function Oxygenases; Multienzyme Complexes; Neurons; Paraventricular Hypothalamic Nucleus; Rats; Rats, Sprague-Dawley; RNA, Messenger; Sensitivity and Specificity; Sulfur Radioisotopes | 1998 |
Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification.
Topics: Biotin; Carcinoma, Transitional Cell; Chromosomes, Human, Pair 1; Chromosomes, Human, Pair 12; Digoxigenin; Fluorescent Dyes; Humans; In Situ Hybridization; Sensitivity and Specificity; Trinitrobenzenes; Tumor Cells, Cultured; Tyramine | 1998 |
Simplified reverse transcription polymerase chain reaction procedure with detection by microplate hybridization for routine screening of hepatitis A virus.
Topics: Animals; Bivalvia; Digoxigenin; DNA Primers; DNA Probes; DNA, Viral; Feces; Hepatitis A; Hepatovirus; Humans; Polymerase Chain Reaction; Sensitivity and Specificity; Shellfish | 1998 |
Detection of thioredoxin in human serum and biological samples using a sensitive sandwich ELISA with digoxigenin-labeled antibody.
Topics: Animals; Antibodies; Asthma; Buffers; Calibration; Digoxigenin; Dose-Response Relationship, Drug; Enzyme-Linked Immunosorbent Assay; Goats; Horseradish Peroxidase; Humans; Hydrogen-Ion Concentration; Indicators and Reagents; Papio; Sensitivity and Specificity; Thioredoxins; Time Factors | 1998 |
A mixture of synthetic oligonucleotide probes labeled with biotin for the sensitive detection of potato spindle tuber viroid.
Topics: Base Sequence; Biotin; Digoxigenin; DNA Probes; DNA, Complementary; Molecular Sequence Data; Nucleic Acid Conformation; Nucleic Acid Hybridization; Oligonucleotide Probes; Plant Viruses; Sensitivity and Specificity; Solanum tuberosum; Viroids | 1998 |
Detection of pathogenic Yersinia enterocolitica using a digoxigenin labelled probe targeting the yst gene.
Topics: Bacterial Toxins; Digoxigenin; DNA Probes; DNA, Ribosomal; Enterotoxins; Genes, Bacterial; RNA, Ribosomal, 16S; Sensitivity and Specificity; Yersinia enterocolitica | 1998 |
Cautionary note on the use of end-labelling DNA fragments for detection of apoptosis.
Topics: Animals; Apoptosis; Deoxyuracil Nucleotides; Dideoxynucleotides; Digoxigenin; Disease Models, Animal; DNA; Female; Ileum; Immunoenzyme Techniques; In Situ Nick-End Labeling; Indicators and Reagents; Rats; Rats, Sprague-Dawley; Sensitivity and Specificity; Skin; Wound Healing | 1998 |
Rapid identification of Candida species with species-specific DNA probes.
Topics: Base Sequence; Candida; Candida albicans; Candidiasis; Digoxigenin; DNA Probes; Evaluation Studies as Topic; Genotype; Humans; Immunoenzyme Techniques; Mycology; Phenotype; Polymerase Chain Reaction; Sensitivity and Specificity; Species Specificity | 1998 |
Comparison of detection techniques for cytokine reverse transcriptase polymerase chain reaction; digoxigenin-labeled polymerase chain reaction permits sensitive detection of cytokine mRNA in rat heart allografts.
Topics: Animals; Blotting, Southern; Digoxigenin; Graft vs Host Disease; Heart Transplantation; Interferon-gamma; Interleukin-2; Lymph Nodes; Myocardium; Rats; Rats, Inbred BN; Rats, Inbred Lew; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sensitivity and Specificity; Specific Pathogen-Free Organisms; Transplantation, Homologous | 1998 |
In situ localization of PCR-amplified DNA and cDNA.
Topics: Biotin; Digoxigenin; DNA, Complementary; DNA, Viral; Endopeptidase K; Female; Gene Dosage; HeLa Cells; Hepatitis C; Humans; In Situ Hybridization; Liver; Measles; Papillomaviridae; Polymerase Chain Reaction; Sensitivity and Specificity; Tumor Cells, Cultured; Uterine Cervical Neoplasms | 1998 |
[Detection of poliovirus by digoxigenin-labeled cDNA probe prepared by PCR technique].
Topics: Digoxigenin; DNA, Complementary; DNA, Viral; Humans; Poliovirus; Polymerase Chain Reaction; Sensitivity and Specificity | 1997 |
Detection of cytokine gene expression in human monocytes and lymphocytes by fluorescent in situ hybridization in cell suspension and flow cytometry.
Topics: Biotin; Digoxigenin; DNA Probes; Flow Cytometry; Gene Expression; Humans; In Situ Hybridization, Fluorescence; Kinetics; Leukocytes, Mononuclear; Lipopolysaccharides; Lymphocytes; Monocytes; Ribonucleases; RNA, Messenger; Sensitivity and Specificity; Sepsis; Stomach Neoplasms; Tumor Necrosis Factor-alpha | 1998 |
Nonradioactive Northern blot analysis of plant RNA and the application of different haptens for reprobing.
Topics: Biotin; Blotting, Northern; Digoxigenin; Fluorescein; Genes, Plant; Haptens; Hordeum; Nucleic Acid Hybridization; Plant Leaves; Polymerase Chain Reaction; Radioactive Tracers; RNA Probes; RNA, Antisense; RNA, Messenger; RNA, Plant; Sensitivity and Specificity; Templates, Genetic; Transcription, Genetic | 1998 |
Nonradioactive detection of differentially expressed genes using complex RNA or DNA hybridization probes.
Topics: Animals; Digoxigenin; DNA, Complementary; Female; Gene Expression Regulation; Genes; Genomic Library; Humans; Langerhans Cells; Luminescent Measurements; Male; Mice; Mice, Inbred BALB C; Molecular Probe Techniques; Nucleic Acid Hybridization; RNA Probes; Sensitivity and Specificity | 1999 |
Chemiluminescent analysis of gene expression on high-density filter arrays.
Topics: Digoxigenin; DNA, Complementary; Gene Expression; Humans; Lymphocytes; RNA-Directed DNA Polymerase; RNA, Messenger; Sensitivity and Specificity | 1999 |
Highly sensitive detection of swine vesicular disease virus based on a single tube RT-PCR system and DIG-ELISA detection.
Topics: Animals; Cells, Cultured; Digoxigenin; DNA Probes; Enterovirus; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Feces; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; Swine; Swine Vesicular Disease; Virus Cultivation | 1999 |
Evaluation of alkaline phosphatase- and digoxigenin-labelled probes for detection of the thermolabile hemolysin (tlh) gene of Vibrio parahaemolyticus.
Topics: Alkaline Phosphatase; Animals; Bacterial Typing Techniques; Digoxigenin; Disease Outbreaks; Environmental Microbiology; Evaluation Studies as Topic; Foodborne Diseases; Genes, Bacterial; Hemolysin Proteins; Humans; Oligonucleotide Probes; Ostreidae; Sensitivity and Specificity; Species Specificity; Temperature; Vibrio Infections; Vibrio parahaemolyticus | 1999 |
Rapid enumeration of Escherichia coli in oysters by a quantitative PCR-ELISA.
Topics: Animals; Bacterial Outer Membrane Proteins; Colony Count, Microbial; Digoxigenin; DNA Primers; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Ostreidae; Polymerase Chain Reaction; Porins; Receptors, Virus; Sensitivity and Specificity | 1999 |
Factor VIII gene inversions and an XbaI polymorphism: nonradioactive detection and clinical usage.
Topics: Adolescent; Adult; Child; Child, Preschool; Chromosome Inversion; Digoxigenin; Factor VIII; Factor VIIIa; Female; Genetic Linkage; Hemophilia A; Humans; Infant; Infant, Newborn; Luminescent Measurements; Male; Middle Aged; Molecular Probes; Polymorphism, Genetic; Sensitivity and Specificity | 1999 |
Identification by PCR of Helicobacter pylori in subgingival plaque of adult periodontitis patients.
Topics: Adult; Blotting, Southern; Dental Plaque; Digoxigenin; DNA, Bacterial; Female; Helicobacter Infections; Helicobacter pylori; Humans; Male; Middle Aged; Periodontitis; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Sensitivity and Specificity; Sequence Analysis, DNA | 1999 |
A non-radioactive method for inexpensive quantitative RT-PCR.
Topics: Base Sequence; Blotting, Southern; Deoxyuracil Nucleotides; Digoxigenin; DNA Primers; DNA, Complementary; Electrophoresis, Agar Gel; Humans; Luminescent Measurements; Receptors, Estrogen; Reference Standards; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sensitivity and Specificity; Tumor Cells, Cultured | 1999 |
Sensitive detection of potato spindle tuber and temperate fruit tree viroids by reverse transcription-polymerase chain reaction-probe capture hybridization.
Topics: Digoxigenin; DNA Probes; DNA, Complementary; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Fruit; Gene Amplification; Genes, Viral; Mosaic Viruses; Nucleic Acid Hybridization; Reverse Transcriptase Polymerase Chain Reaction; RNA Probes; RNA, Viral; Sensitivity and Specificity; Solanum tuberosum; Viroids | 1999 |
Nonisotopic detection of Loma salmonae (microspora) in rainbow trout (Oncorhynchus mykiss) gills by in situ hybridization.
Topics: Animals; Digoxigenin; DNA Probes; DNA, Protozoan; DNA, Single-Stranded; Fish Diseases; Gills; In Situ Hybridization; Microsporida; Microsporidiosis; Oncorhynchus mykiss; Polymerase Chain Reaction; Sensitivity and Specificity | 1999 |
Immobilon-Ny+ nucleic acid blotting membrane: an advanced nylon membrane optimized for superior fixation and reprobing.
Topics: Blotting, Northern; Blotting, Southern; Digoxigenin; DNA Probes; Luminescent Measurements; Nucleic Acids; Phosphorus Radioisotopes; RNA Probes; Sensitivity and Specificity | 1999 |
High-density hapten labeling and HRP conjugation of oligonucleotides for use as in situ hybridization probes to detect mRNA targets in cells and tissues.
Topics: Biotin; Chorionic Villi; Digoxigenin; Fetus; Fluorescein; Haptens; Hemoglobins; Horseradish Peroxidase; Humans; In Situ Hybridization; Liver; Oligonucleotide Probes; Sensitivity and Specificity | 2000 |
Digoxigenin-end labeled oligonucleotides facilitate the detection of minimal residual disease in acute lymphoblastic leukemia.
Topics: Adult; Autoradiography; Digoxigenin; DNA, Neoplasm; Enzyme-Linked Immunosorbent Assay; Gene Rearrangement, B-Lymphocyte, Light Chain; Gene Rearrangement, delta-Chain T-Cell Antigen Receptor; Genes, Immunoglobulin; Humans; Luminescent Measurements; Neoplasm Proteins; Neoplasm, Residual; Nucleic Acid Hybridization; Polymerase Chain Reaction; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Reagent Kits, Diagnostic; Sensitivity and Specificity | 2000 |
Use of an alkaline phosphatase-labelled probe for the detection of Mycoplasma synoviae in chickens.
Topics: Alkaline Phosphatase; Animals; Chickens; Colorimetry; Digoxigenin; DNA Probes; DNA, Bacterial; Female; Mycoplasma; Mycoplasma Infections; Nucleic Acid Hybridization; Polymerase Chain Reaction; Poultry Diseases; Sensitivity and Specificity | 2000 |
Competitive cytokeratin 19 RT-PCR for quantification of breast cancer cells in blood cell suspensions.
Topics: Antibodies; Binding, Competitive; Blood Specimen Collection; Breast Neoplasms; Digoxigenin; DNA Primers; DNA Probes; Enzyme-Linked Immunosorbent Assay; Female; Humans; Keratins; Leukocytes, Mononuclear; Pseudogenes; Reference Standards; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; RNA, Neoplasm; Sensitivity and Specificity | 2000 |
Improved nonradioactive RT-PCR method for relative quantification of mRNA.
Topics: Antisense Elements (Genetics); Deoxyuracil Nucleotides; Digoxigenin; Receptors, Progesterone; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Sensitivity and Specificity | 2000 |
Long-depth imaging of specific gene expressions in whole-mount mouse embryos with single-photon excitation confocal fluorescence microscopy and FISH.
Topics: Animals; Antisense Elements (Genetics); Central Nervous System; Digoxigenin; DNA-Binding Proteins; Embryo, Mammalian; Eye Proteins; Fluorescein-5-isothiocyanate; Fluorescent Antibody Technique; Gene Expression; Gene Expression Profiling; Genes, myc; Homeodomain Proteins; Image Enhancement; In Situ Hybridization, Fluorescence; Mice; Microscopy, Confocal; Microscopy, Fluorescence; Organ Specificity; Paired Box Transcription Factors; PAX6 Transcription Factor; Repressor Proteins; RNA Probes; RNA, Messenger; Scattering, Radiation; Sensitivity and Specificity; Time Factors | 2000 |
A double labeling technique using WGA-apoHRP-gold as a retrograde tracer and non-isotopic in situ hybridization histochemistry for the detection of mRNA.
Topics: Afferent Pathways; Amygdala; Animals; Axonal Transport; Brain Stem; Digoxigenin; Glutamate Decarboxylase; Immunoenzyme Techniques; In Situ Hybridization; Injections; Macaca fascicularis; Nerve Tissue Proteins; RNA, Messenger; Sensitivity and Specificity; Silver Staining; Specimen Handling; Stereotaxic Techniques; Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate | 2000 |
Screening sandflies for natural infection with Leishmania donovani, using a non-radioactive probe based on the total DNA of the parasite.
Topics: Animals; Digoxigenin; DNA Probes; Female; Leishmania donovani; Psychodidae; Sensitivity and Specificity | 2000 |
A new DIG-PCR-EIA method for the detection of Chlamydia pneumoniae DNA in clinical samples.
Topics: Adenoids; Adult; Biotinylation; Blotting, Southern; Carrier State; Child; Chlamydia Infections; Chlamydophila pneumoniae; Digoxigenin; DNA Probes; DNA, Bacterial; Ear, Middle; Humans; Immunoenzyme Techniques; Otitis Media; Pharynx; Polymerase Chain Reaction; Respiratory Tract Infections; Sensitivity and Specificity; Streptavidin | 2000 |
Sensitive and nonradioactive in situ detection of neurotrophin mRNAs in the nervous system.
Topics: Animals; Digoxigenin; Ganglia; Immunoenzyme Techniques; In Situ Hybridization; Nerve Growth Factors; Nerve Tissue; Nucleic Acid Hybridization; Rats; RNA Probes; RNA, Messenger; Sensitivity and Specificity; Transcription, Genetic | 2001 |
Double in situ hybridization for simultaneous detection of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus (PCV).
Topics: Animals; Circoviridae Infections; Circovirus; Digoxigenin; DNA, Viral; In Situ Hybridization; Lung; Macrophages, Alveolar; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Sensitivity and Specificity; Swine | 2001 |
A system to enhance the sensitivity of digoxigenin-labelled probe: detection of B19 DNA in serum samples.
Topics: Digoxigenin; DNA Probes; DNA, Viral; Humans; Nucleic Acid Amplification Techniques; Parvoviridae Infections; Parvovirus; Sensitivity and Specificity | 2001 |
In situ detection of messenger RNA using digoxigenin-labeled oligonucleotides and rolling circle amplification.
Topics: 5' Untranslated Regions; Actins; Animals; Base Sequence; Cell Line; Digoxigenin; DNA Probes; Image Processing, Computer-Assisted; Immunoglobulin G; In Situ Hybridization; In Situ Hybridization, Fluorescence; Liver; Microscopy; Molecular Sequence Data; Oligodeoxyribonucleotides, Antisense; Oligonucleotide Probes; Rats; RNA, Messenger; Sensitivity and Specificity | 2001 |
Detection of adeno-associated virus DNA in female genital samples by PCR-ELISA.
Topics: Cervix Uteri; Dependovirus; Digoxigenin; DNA, Viral; Enzyme-Linked Immunosorbent Assay; Female; Genitalia, Female; Humans; Italy; Papillomaviridae; Papillomavirus Infections; Parvoviridae Infections; Polymerase Chain Reaction; Sensitivity and Specificity; Streptavidin; Tumor Virus Infections | 2001 |
Non-radioactive digoxigenin DNA labeling and immunologic detection of HSV PCR products.
Topics: Culture Media; Digoxigenin; DNA, Viral; Herpes Simplex; Humans; Immunoassay; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Simplexvirus; Staining and Labeling | 2001 |
Herpes-like virus detection in infected Crassostrea gigas spat using DIG-labelled probes.
Topics: Animals; Blotting, Southern; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Viral; Herpesviridae; In Situ Hybridization; Ostreidae; Pacific Ocean; Polymerase Chain Reaction; Sensitivity and Specificity | 2002 |
A reverse-transcription competitive PCR assay based on chemiluminescence hybridization for detection and quantification of hepatitis C virus RNA.
Topics: Branched DNA Signal Amplification Assay; Calibration; Digoxigenin; Hepacivirus; Hepatitis C; Hepatitis C Antibodies; Humans; Luminescent Measurements; Reference Standards; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Sensitivity and Specificity; Viremia | 2002 |
Sensitive two-color whole-mount in situ hybridizations using digoxygenin- and dinitrophenol-labeled RNA probes.
Topics: Animals; Antisense Elements (Genetics); Color; Digoxigenin; Dinitrophenols; Fetal Proteins; Gene Expression Regulation, Developmental; In Situ Hybridization; Intracellular Signaling Peptides and Proteins; Left-Right Determination Factors; RNA Probes; Sensitivity and Specificity; T-Box Domain Proteins; Transforming Growth Factor beta; Zebrafish; Zebrafish Proteins | 2002 |
In situ detection of Bartonella henselae cells.
Topics: Animals; Bacterial Typing Techniques; Bartonella henselae; Bartonella Infections; Digoxigenin; DNA Probes; Fluorescein; Fluorescent Dyes; In Situ Hybridization; Liver; Male; Mice; Mice, Inbred BALB C; Microscopy, Fluorescence; Sensitivity and Specificity; Spleen | 2002 |
High-sensitivity hybridization assay for quantitation of residual E. coli DNA.
Topics: Antibodies; Buffers; Digoxigenin; DNA Probes; DNA, Bacterial; Escherichia coli; Luminescent Measurements; Nucleic Acid Hybridization; Recombinant Proteins; Sensitivity and Specificity; Serum Albumin, Bovine | 2002 |
Digoxigenin as an alternative probe labeling for in situ hybridization.
Topics: Digoxigenin; Drug Stability; Evaluation Studies as Topic; Humans; In Situ Hybridization; Molecular Probe Techniques; Parathyroid Glands; Parathyroid Hormone; RNA, Messenger; Sensitivity and Specificity | 1992 |
Evaluation of a colorimetric Babesia bigemina-DNA probe within an epidemiological survey.
Topics: Age Factors; Animals; Antibodies, Protozoan; Babesia; Babesiosis; Carrier State; Cattle; Cattle Diseases; Colorimetry; Digoxigenin; DNA Probes; DNA, Protozoan; Fluorescent Antibody Technique; Mexico; Nucleic Acid Hybridization; Prevalence; Sensitivity and Specificity | 1992 |
'Run-off' polymerization with digoxigenin labelled nucleotides creates highly sensitive and strand specific DNA hybridization probes: synthesis and application.
Topics: Actins; Base Sequence; Blotting, Northern; Cells, Cultured; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Single-Stranded; Electrophoresis, Agar Gel; Enterovirus B, Human; Genetic Vectors; Humans; Immunoblotting; Indicators and Reagents; Molecular Sequence Data; Platelet-Derived Growth Factor; Polymers; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-sis; RNA; Sensitivity and Specificity | 1992 |
Detection of Mycobacterium tuberculosis in clinical samples by using polymerase chain reaction and a nonradioactive detection system.
Topics: Base Sequence; Digoxigenin; DNA, Bacterial; Humans; Molecular Sequence Data; Mycobacterium tuberculosis; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Sputum; Tuberculosis | 1992 |
Polymerase chain reaction for detection of Leptospira spp. in clinical samples.
Topics: Adolescent; Adult; Aged; Animals; Base Sequence; Blood; Blotting, Southern; Digoxigenin; DNA, Ribosomal; Female; Humans; Leptospira interrogans; Male; Mice; Middle Aged; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; RNA, Ribosomal, 16S; Sensitivity and Specificity; Species Specificity; Spirochaetales; Urine; Weil Disease | 1992 |
Generation of digoxigenin-labelled double-stranded and single-stranded probes using the polymerase chain reaction.
Topics: Base Sequence; Blotting, Southern; Cells, Cultured; Digoxigenin; DNA; DNA Probes; DNA, Single-Stranded; DNA, Viral; Hepatitis B; Hepatitis B virus; Humans; Liver; Molecular Sequence Data; Polymerase Chain Reaction; Sensitivity and Specificity | 1992 |
Identification of Shiga-like toxin type II producing Escherichia coli using the polymerase chain reaction and a digoxigenin labelled DNA probe.
Topics: Bacterial Toxins; Blotting, Southern; Digoxigenin; DNA Probes; Enterotoxins; Escherichia coli; Escherichia coli Infections; Genes, Bacterial; Humans; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Shiga Toxin 2 | 1992 |
Quantification of sensitive non-isotopic filter hybridizations using the peroxidase catalyzed luminol reaction.
Topics: Biotin; Biphenyl Compounds; Blotting, Southern; Dextran Sulfate; Digoxigenin; DNA; Firefly Luciferin; Horseradish Peroxidase; Iodobenzenes; Luminescent Measurements; Luminol; Nucleic Acid Hybridization; Photography; Polyethylene Glycols; Reproducibility of Results; Sensitivity and Specificity; Thiazoles; Ultrafiltration | 1992 |
Highly sensitive digoxigenin-labelled DNA probe for the detection of potato spindle tuber viroid.
Topics: Digoxigenin; DNA Probes; Evaluation Studies as Topic; Fruit; Molecular Probe Techniques; Nucleic Acid Hybridization; Plant Viruses; RNA, Viral; Sensitivity and Specificity; Solanum tuberosum; Virology | 1992 |
Nonradioactive polynucleotide gene probe assay for the detection of three virulence toxin genes in diarrhoeal stools.
Topics: Child; Diarrhea; Digoxigenin; DNA Probes; Enterotoxins; Escherichia coli; Escherichia coli Infections; Feces; Genes, Bacterial; Humans; Nucleic Acid Hybridization; Sensitivity and Specificity; Virulence | 1992 |
Detection of rabies virus mRNA in mouse brain by using in situ hybridization with digoxigenin-labelled RNA probes.
Topics: Animals; Brain; Deoxyuracil Nucleotides; Digoxigenin; Female; Glycoproteins; Mice; Mice, Inbred ICR; Rabies; Rabies virus; RNA Probes; RNA, Messenger; RNA, Viral; Sensitivity and Specificity; Tritium | 1992 |
Nonradioactive in situ hybridization using digoxigenin-labeled oligonucleotides. Applications to musculoskeletal tissues.
Topics: Animals; Animals, Newborn; Base Sequence; Cartilage, Articular; Chickens; Digoxigenin; Growth Plate; Molecular Sequence Data; Musculoskeletal System; Nucleic Acid Hybridization; Oligonucleotide Probes; RNA, Messenger; Sensitivity and Specificity | 1992 |
Detection of HIV1 proviral DNA by PCR and hybridization with digoxigenin labelled probes.
Topics: Base Sequence; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Viral; HIV-1; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Proviruses; Sensitivity and Specificity | 1992 |
Nonradioactive in situ hybridization with digoxigenin labeled DNA probes.
Topics: Animals; Biotin; Cell Nucleus; Chromosomes; Digoxigenin; DNA Probes; Humans; Liver Neoplasms, Experimental; Nucleic Acid Hybridization; Sensitivity and Specificity; Tumor Cells, Cultured; Y Chromosome | 1992 |
A sensitive microplate assay for glycoproteins that utilizes an immunological digoxigenin-based detection system.
Topics: Animals; Cattle; Digoxigenin; Glycoproteins; Humans; Microchemistry; Polysaccharides; Reagent Kits, Diagnostic; Reproducibility of Results; Sensitivity and Specificity; Swine | 1992 |
Optimization of non-isotopic in situ hybridization on formalin-fixed, paraffin-embedded material using digoxigenin-labelled probes and transgenic tissues.
Topics: alpha 1-Antitrypsin; Animals; Digoxigenin; Formaldehyde; Liver; Mice; Mice, Transgenic; Molecular Probe Techniques; Nucleic Acid Hybridization; Paraffin Embedding; Sensitivity and Specificity; Tissue Fixation | 1992 |
Sensitive non-isotopic DNA hybridisation assay or immediate-early antigen detection for rapid identification of human cytomegalovirus in urine.
Topics: Antigens, Viral; Base Sequence; Cytomegalovirus; Cytomegalovirus Infections; Digoxigenin; DNA Probes; DNA, Viral; Endopeptidase K; Humans; Immediate-Early Proteins; Molecular Sequence Data; Nucleic Acid Hybridization; Polymerase Chain Reaction; Sensitivity and Specificity; Serine Endopeptidases | 1991 |
Use of M13 single-stranded DNA digoxigenin labelled probe for detection of human parvovirus B19 viraemia.
Topics: Base Sequence; Digoxigenin; DNA Probes; Humans; Molecular Sequence Data; Parvovirus B19, Human; Sensitivity and Specificity | 1991 |
Chemiluminescence dot blot hybridization assay for detection of B19 parvovirus DNA in human sera.
Topics: Digoxigenin; DNA Probes; DNA, Viral; Erythema Infectiosum; Humans; Immunoblotting; Luminescent Measurements; Nucleic Acid Hybridization; Parvovirus B19, Human; Sensitivity and Specificity | 1991 |
Digoxygenin-labelled DNA-probe: a rapid non-radioactive method for hepatitis B virus DNA detection in serum.
Topics: Alanine Transaminase; Chronic Disease; Digoxigenin; DNA Probes; DNA, Viral; False Positive Reactions; Hepatitis B; Hepatitis B e Antigens; Hepatitis B Surface Antigens; Hepatitis B virus; Hepatitis C; Humans; Sensitivity and Specificity; Virus Replication | 1991 |
Detection of mRNA and hnRNA using a digoxigenin labelled cDNA probe by in situ hybridization on frozen tissue sections.
Topics: Child, Preschool; Digoxigenin; DNA Probes; Frozen Sections; Humans; Infant; Lymph Nodes; Nucleic Acid Hybridization; Receptors, Antigen, T-Cell, alpha-beta; RNA, Heterogeneous Nuclear; RNA, Messenger; Sensitivity and Specificity; T-Lymphocytes; Thymus Gland | 1991 |
A comparison of non-radioactive labeling and detection systems with synthetic oligonucleotide probes for the species identification of mosquitoes in the Anopheles gambiae complex.
Topics: Animals; Anopheles; Base Sequence; Biotin; Costs and Cost Analysis; Digoxigenin; Horseradish Peroxidase; Insect Vectors; Luminescent Measurements; Male; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Sensitivity and Specificity | 1991 |
Rapid confirmation of Listeria monocytogenes isolated from foods by a colony blot assay using a digoxigenin-labeled synthetic oligonucleotide probe.
Topics: Bacterial Proteins; Base Sequence; Cloning, Molecular; Digoxigenin; DNA, Bacterial; Food Microbiology; Listeria monocytogenes; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Plasmids; Sensitivity and Specificity | 1991 |
Detection of HBV-DNA using a digoxigenin-labelled probe. A rapid technique without loss of sensitivity.
Topics: Digoxigenin; DNA Probes; DNA, Viral; Hepatitis B virus; Humans; Nucleic Acid Hybridization; Phosphorus Radioisotopes; Sensitivity and Specificity; Time Factors | 1991 |
Digoxigenin-labeled probes for the detection of hepatitis B virus DNA in serum.
Topics: Digoxigenin; DNA Probes; DNA, Viral; Evaluation Studies as Topic; Hepatitis B; Hepatitis B virus; Humans; Nucleic Acid Hybridization; Phosphorus Radioisotopes; Sensitivity and Specificity; Virus Replication | 1991 |
Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase.
Topics: Biotin; Blotting, Southern; Digoxigenin; DNA; Haptens; Oligonucleotides; Sensitivity and Specificity; Transferases | 1991 |
Characterization of an rRNA gene-specific cDNA probe: applications in bacterial identification.
Topics: Alkaline Phosphatase; Campylobacter jejuni; Deoxyuracil Nucleotides; Digoxigenin; DNA Probes; DNA, Bacterial; Oligonucleotides; Pasteurella; RNA, Ribosomal; RNA, Ribosomal, 16S; RNA, Ribosomal, 23S; Sensitivity and Specificity | 1990 |
Detection of cytomegalovirus DNA using probes labelled with digoxigenin.
Topics: Biotin; Complement Fixation Tests; Cytomegalovirus; Cytomegalovirus Infections; Digoxigenin; Digoxin; DNA Probes; DNA, Viral; Evaluation Studies as Topic; Humans; Sensitivity and Specificity | 1989 |