digitonin and Astrocytoma

digitonin has been researched along with Astrocytoma* in 2 studies

Other Studies

2 other study(ies) available for digitonin and Astrocytoma

Article	Year
Maintenance of whole cell isoproterenol and forskolin responsiveness in adenylate cyclase of permeabilized cells. Journal of cyclic nucleotide and protein phosphorylation research, 1986, Volume: 11, Issue:2 Digitonin-permeabilized monolayers of C6-2B rat astrocytoma cells exhibit adenylate cyclase activity in the presence of exogenously added ATP. The adenylate cyclase retains the qualitative and quantitative characteristics of hormone stimulated cyclic AMP accumulation in whole cells including GTP dependency and 100 fold stimulation by isoproterenol. Forskolin increased enzymatic activity in the absence of added GTP, however forskolin efficacy and potency was enhanced by GTP. Low non-efficacious concentrations of forskolin, without added GTP, supported isoproterenol-stimulated cyclase activity. The GTP-stimulated isoproterenol response was potentiated by forskolin. Forskolin support of isoproterenol stimulated cyclase in the absence of GTP raises the possibility that forskolin can act independently of GTP in coupling receptors to cyclase catalytic units and/or that forskolin could increase the efficacy and potency of GTP in the coupling reaction. Permeabilization of C6-2B and other cultured cells yields a preparation of adenylate cyclase which retains the enzyme in a state which closely approximates its activity in the native membrane--a system which could prove useful in studies of the regulation of adenylate cyclase in vivo. Topics: Adenosine Triphosphate; Adenylyl Cyclases; Animals; Astrocytoma; Cell Line; Cell Membrane Permeability; Colforsin; Digitonin; Guanosine Triphosphate; Isoproterenol; Kinetics; Rats	1986
[The ultrastructural localization and distribution of free cholesterol (3-beta-hydroxisterol) in human cerebral tumours (author's transl)]. Acta histochemica, 1976, Volume: 55, Issue:1 According to the examination of the ultrastructure of the human gliomas and the ultrastructural localization of their free cholesterol, it can be established that the main part of the free cholesterol is present in the cells in a structural fixed form and that multiplication of the free cholesterol, occurring due to cell degeneration in the vacuoles and cysts, can be brought in connection with the disintegration of these structures. Topics: Astrocytoma; Brain Neoplasms; Cholesterol; Digitonin; Glioblastoma; Histocytochemistry; Humans; Microscopy, Electron; Oligodendroglioma; Staining and Labeling	1976

Article

Year

Maintenance of whole cell isoproterenol and forskolin responsiveness in adenylate cyclase of permeabilized cells.

Journal of cyclic nucleotide and protein phosphorylation research, 1986, Volume: 11, Issue:2

Digitonin-permeabilized monolayers of C6-2B rat astrocytoma cells exhibit adenylate cyclase activity in the presence of exogenously added ATP. The adenylate cyclase retains the qualitative and quantitative characteristics of hormone stimulated cyclic AMP accumulation in whole cells including GTP dependency and 100 fold stimulation by isoproterenol. Forskolin increased enzymatic activity in the absence of added GTP, however forskolin efficacy and potency was enhanced by GTP. Low non-efficacious concentrations of forskolin, without added GTP, supported isoproterenol-stimulated cyclase activity. The GTP-stimulated isoproterenol response was potentiated by forskolin. Forskolin support of isoproterenol stimulated cyclase in the absence of GTP raises the possibility that forskolin can act independently of GTP in coupling receptors to cyclase catalytic units and/or that forskolin could increase the efficacy and potency of GTP in the coupling reaction. Permeabilization of C6-2B and other cultured cells yields a preparation of adenylate cyclase which retains the enzyme in a state which closely approximates its activity in the native membrane--a system which could prove useful in studies of the regulation of adenylate cyclase in vivo.

Topics: Adenosine Triphosphate; Adenylyl Cyclases; Animals; Astrocytoma; Cell Line; Cell Membrane Permeability; Colforsin; Digitonin; Guanosine Triphosphate; Isoproterenol; Kinetics; Rats

1986

[The ultrastructural localization and distribution of free cholesterol (3-beta-hydroxisterol) in human cerebral tumours (author's transl)].

Acta histochemica, 1976, Volume: 55, Issue:1

According to the examination of the ultrastructure of the human gliomas and the ultrastructural localization of their free cholesterol, it can be established that the main part of the free cholesterol is present in the cells in a structural fixed form and that multiplication of the free cholesterol, occurring due to cell degeneration in the vacuoles and cysts, can be brought in connection with the disintegration of these structures.

Topics: Astrocytoma; Brain Neoplasms; Cholesterol; Digitonin; Glioblastoma; Histocytochemistry; Humans; Microscopy, Electron; Oligodendroglioma; Staining and Labeling

1976