difluprednate and Foot-and-Mouth-Disease

An efficient method for detection of foot and mouth disease (FMD) and, particularly, differentiation of vaccinated from infected animals is the use of nonstructural (NS) proteins as antigens in Enzyme-Linked Immunosorbent Assay (ELISA) Kits. In this study, only epitopic regions of 3AB and 3D NS proteins were used for recombinant protein production, as a cost-effective method instead of peptide synthesis, for application in in-house ELISA diagnostic kits. Specific primers were designed according to the antigenic regions of 3AB (C-terminus of 3A and the whole 3B) and 3D (N-terminus) proteins, and the polymerase chain reaction (PCR) amplification was performed. Purified amplicons were cloned into pET21a (+) vectors and then transformed into

Topics: Animals; Antibodies, Viral; Enzyme-Linked Immunosorbent Assay; Fluprednisolone; Foot-and-Mouth Disease; Foot-and-Mouth Disease Virus; Recombinant Proteins; Sensitivity and Specificity; Viral Nonstructural Proteins