dieckol and Body-Weight

In the present study, the attenuation of type ІІ diabetes by dieckol, a phlorotannin derivative isolated from brown seaweed, Ecklonia cava was investigated in C57BL/KsJ-db/db, a type ІІ diabetes mouse model. Dieckol was administered intraperitoneal injection at doses of 10 and 20 mg/kg body weight diabetes mice for 14 days. The blood glucose level, serum insulin level and body weight were significantly reduced in the dieckol administered group, compared to that of the saline administered group. Furthermore, reduced thiobarbituric acid reactive substraces (TBARS), as well as increased activities of antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-px) in liver tissues were observed in the dieckol administered group. In addition, increased levels of the phosphorylation of AMPK and Akt were observed in the muscle tissues of the dieckol administered group in a Western blotting analysis. According to the findings of this study, it could be suggested that, dieckol can be developed as a therapeutic agent for type ІІ diabetes.

Topics: AMP-Activated Protein Kinases; Animals; Benzofurans; Blood Glucose; Body Weight; Catalase; Diabetes Mellitus, Experimental; Disease Models, Animal; Glutathione Peroxidase; Hypoglycemic Agents; Insulin; Lipid Peroxidation; Mice; Mice, Inbred C57BL; Phaeophyceae; Phosphorylation; Proto-Oncogene Proteins c-akt; Seaweed; Signal Transduction; Superoxide Dismutase

Alcoholic liver disease, which is one of the most serious liver disorders, has been known to cause by ethanol intake. In the present study, in vivo hepatoprotective effects of dieckol-rich phlorotannins (DRP) from Ecklonia cava, a brown seaweed, on ethanol induced hepatic damage in BALB/c mice liver were investigated. After administration of 5 and 25mg/kg mouse of DRP and 4 g/kg mice ethanol, the body weights and survival rates were increased as compared to the control, which is ethanol-treated group without DRP. The glutamic oxaloacetic transaminase and glutamic pyruvic transaminase levels in the serum were lower than those of the control. DRP exhibited a reduction of the total cholesterol. The lower levels of SOD enzyme and a reduction of the formation of malondialdehyde were occurred in mice fed with 5 and 25mg/kg mouse of DRP. Finally the effect on improvement of fatty liver induced by ethanol was observed by taking out the liver immediately after dissecting the mouse. However, no significant difference was observed on hepatic histopathological changes. In conclusion, this study indicated that DRP could protect liver injury induced by ethanol in vivo. It suggested that DRP possesses the beneficial effect to human against ethanol-induced liver injury.

Topics: Alanine Transaminase; Animals; Aspartate Aminotransferases; Benzofurans; Body Weight; Cholesterol; Glutathione Peroxidase; Hepatitis, Alcoholic; Lipid Peroxidation; Liver; Male; Malondialdehyde; Mice; Mice, Inbred BALB C; Republic of Korea; Seaweed; Survival; Tannins; Thiobarbituric Acid Reactive Substances