dicumarol and Inflammation

Four functional assays for protein S were evaluated by 4 different laboratories, each center using its own method. The aim of this study was to compare these different assays and to establish a relationship with results of immunological assays of total and free protein S antigen and C4bBP. The same plasma samples were distributed to each center and tested in blind. In 47 normal subjects, there was no significant difference between the 4 functional assays, with mean values ranging from 93 to 100%. These values were in good agreement with those of free and total protein S antigen. In 34 patients with a quantitative congenital deficiency of protein S the mean values of protein S activity were decreased with the 4 assays, ranging from 25 to 40%. Free protein S antigen was reduced to a similar extent, whereas total antigen was either normal or decreased. The correlation of protein S activity with free protein S antigen was satisfactory for 3 methods, with coefficients of correlation varying from 0.84 to 0.92 whereas it was only 0.70 in one lab. When total protein S antigen was reduced, protein S activity was decreased in all the patients with the 4 assays. In contrast when total protein S antigen was normal an important overlap of protein S activity between normals and patients was observed in one lab with 12 patients misclassified. In 8 patients with a functional defect, results of protein S activity differed substantially according to the assay used and about half of these patients were misclassified.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Administration, Oral; Biological Assay; Dicumarol; Europe; Female; Humans; Infant, Newborn; Inflammation; Male; Protein S; Protein S Deficiency; Reproducibility of Results

A new one-step ELISA using two monoclonal antibodies specific for distinct epitopes of the free form of protein S (ELISA-m) has been developed for the direct measurement of free protein S in untreated plasma. This assay has been compared with the classic method using polyclonal antibodies to protein S (ELISA-p). The latter method has the drawback of requiring PEG precipitation of plasma which is time-consuming, difficult to perform with accuracy and therefore poorly reproducible in most laboratories. Results of both ELISAs were compared with those of a functional assay. In 30 normal subjects, there was an excellent correlation between ELISA-m and ELISA-p (r = 0.95) as well as between ELISA-m and the functional assay (r = 0.96). In twelve patients with a congenital deficiency, the levels of free protein S antigen were similarly decreased with ELISA-m and ELISA-p and in good agreement with those of protein S activity. In 20 patients with miscellaneous inflammatory diseases, the levels of free proteins S were normal with good correlation between both ELISAs and PS activity, despite high levels of C4bBP-protein S complexes. As expected, in 15 dicoumarol-treated patients, there was a significant and parallel decrease of free protein S antigen with both ELISAs, with even lower levels of protein S activity. In 14 patients with liver cirrhosis, the mean values for free protein S antigen were normal using both assays, but with wide extreme values, whereas protein S activity was significantly lower.(ABSTRACT TRUNCATED AT 250 WORDS)

Topics: Antibodies, Monoclonal; Dicumarol; Enzyme-Linked Immunosorbent Assay; Female; Humans; Inflammation; Liver Cirrhosis; Male; Protein S; Protein S Deficiency; Reference Values

Topics: Anti-Inflammatory Agents; Aspirin; Blood Platelets; Cell Membrane; Chloroquine; Coumarins; Depression, Chemical; Dicumarol; Erythrocytes; Fibrinolysis; Flufenamic Acid; Hemolysis; Humans; Hypotonic Solutions; In Vitro Techniques; Indomethacin; Inflammation; Mefenamic Acid; Oxyphenbutazone; Phenylbutazone; Phenylpropionates; Platelet Adhesiveness; Pyrazoles; Receptors, Drug; Structure-Activity Relationship

Topics: Adenosine Triphosphate; Adrenocorticotropic Hormone; Anti-Inflammatory Agents; Antipyrine; Autoradiography; Benzoates; Cartilage; Chloroquine; Chromatography; Dicumarol; Dinitrophenols; Glycosaminoglycans; Hydrocortisone; Inflammation; Kidneys, Artificial; Metabolism; ortho-Aminobenzoates; Oxyphenbutazone; Phenylbutazone; Pyrazoles; Quinolines; Rats; Research; Sodium Salicylate; Sulfates; Sulfinpyrazone; Sulfur Isotopes; Uricosuric Agents; Urine

Topics: Animals; Blood Coagulation; Blood Proteins; Dicumarol; Edema; Epilepsy; Epilepsy, Post-Traumatic; Escherichia coli Infections; Fibrin; Heparin; Inflammation; Leukocytes; Pharmacology; Rabbits; Rats; Research; Skin; Sulfur Isotopes