dicumarol and Blood-Coagulation-Disorders

Topics: Acetylcholine; Antimetabolites; Aspirin; Autonomic Nervous System; Blood Circulation; Blood Coagulation Disorders; Blood Coagulation Factors; Blood Platelets; Calcium; Cell Aggregation; Clot Retraction; Dicumarol; Epinephrine; Factor IX; Factor V; Factor VII; Factor VIII; Factor X; Fibrin; Fibrinogen; Hemorrhage; Hemostasis; Humans; Prothrombin; Prothrombin Time; Surgical Procedures, Operative; Thrombin; Vasoconstrictor Agents; Vasodilator Agents; Vitamin K

Topics: Adult; Androgens; Antithrombin III; Antithrombin III Deficiency; Blood Coagulation Disorders; Child; Dicumarol; Disseminated Intravascular Coagulation; Female; Humans; Male; Pregnancy

Topics: Aortic Valve; Blood Coagulation Disorders; Dicumarol; Follow-Up Studies; Heart Valve Prosthesis; Heparin; Humans; Mitral Valve; Postoperative Complications; Tricuspid Valve

A three-step spectrophotometric assay was developed for measuring functional protein C (PC) in human plasma. The assay is based on: (1) adsorption of citrated platelet-poor plasma on barium citrate and elution of the vitamin K-dependent factors with EDTA; (2) activation of PC by incubation of the mixture of vitamin K-dependent factors with a complex of thrombin and its endothelial cell cofactor, thrombomodulin; (3) addition of antithrombin III and heparin to the system to inhibit thrombin and other coagulation enzymes generated during incubation and measurement of the activated PC with a synthetic (chromogenic) substrate. The assay appears to be specific for PC because: (a) PC-depleted plasma (by immunoadsorption) is inactive; (b) addition of purified PC to PC-depleted plasma reconstitutes its activity; and (c) no enzymatic activity is generated in the absence of the thrombin-thrombomodulin complex. Mixtures of a normal plasma pool with PC-depleted plasma yielded an amount of enzymatic activity proportional to the fraction of normal plasma. Using this as a standard curve, the amount of PC in the plasma of 23 normal subjects was 97% +/- 15%. The within-assay coefficient of variation was 3.5% and the between-assay coefficient 6.5%. A linear correlation (r = 0.86) was found between PC as measured with the functional assay and with a radioimmunoassay. In 3 patients with congenital PC deficiency, the functional PC level was 37% +/- 9% and the antigen level 64% +/- 11%. It is concluded that the present assay may be used for reliable and accurate estimation of activatable PC in human plasma.

Topics: Adsorption; Blood Coagulation Disorders; Blood Coagulation Factors; Blood Coagulation Tests; Chromogenic Compounds; Dicumarol; Glycoproteins; Humans; Protein C; Receptors, Cell Surface; Receptors, Thrombin; Thrombin

Topics: Animals; Blood Coagulation; Blood Coagulation Disorders; Dicumarol; Factor X Deficiency; Factor XI Deficiency; Factor XIII Deficiency; Hemophilia B; Hemorrhage; Hemostasis; Heparin; Horse Diseases; Horses; Models, Biological

Topics: Blood Coagulation Disorders; Dicumarol; Fibrin Fibrinogen Degradation Products; Germany, West; Hematology; History of Medicine; Humans

Topics: Animals; Blood Coagulation; Blood Coagulation Disorders; Blood Coagulation Factors; Cattle; Cattle Diseases; Denmark; Dicumarol; Dogs; Factor IX; Factor V; Factor VII; History, 20th Century; Humans; Plant Poisoning; Prothrombin; Rats; United States; Vitamin K

Topics: Amino Acids; Blood Coagulation Disorders; Blood Coagulation Tests; Chromatography, Gas; Chromatography, Ion Exchange; Dicumarol; Electrophoresis, Polyacrylamide Gel; Galactose; Glucosamine; Glucose; Humans; Immunoelectrophoresis; Mannose; Neuraminic Acids; Prothrombin

Topics: Adult; Aged; Anticoagulants; Aortic Aneurysm; Arteriosclerosis; Arteritis; Blood Coagulation Disorders; Blood Coagulation Tests; Diabetic Angiopathies; Dicumarol; Embolism; Female; Humans; Male; Middle Aged; Raynaud Disease; Thrombophlebitis; Thrombosis; Vascular Diseases

Topics: Adult; Antithrombins; Appendectomy; Blood Coagulation Disorders; Blood Transfusion; Cholecystectomy; Dicumarol; Female; Heparin; Humans; Preoperative Care; Thrombosis

Topics: Ammonium Sulfate; Animals; Barium; Barium Sulfate; Biological Assay; Blood Coagulation Disorders; Blood Coagulation Tests; Cattle; Chromatography; Chromatography, Gel; Chromatography, Ion Exchange; Citrates; Cross Reactions; Dicumarol; Electrophoresis, Disc; Enzyme Activation; Epitopes; Immunoelectrophoresis; Male; Prothrombin; Rabbits; Vitamin K

Topics: Amino Acids; Animals; Binding Sites; Blood Coagulation Disorders; Calcium; Carbohydrates; Cattle; Chromatography, Gel; Chromatography, Ion Exchange; Dicumarol; Electrophoresis, Disc; Enzyme Activation; Factor X; Female; Hydrogen-Ion Concentration; Precipitin Tests; Prothrombin; Rabbits; Snakes; Spectrophotometry, Ultraviolet; Trypsin; Venoms; Vitamin K

Topics: Alkylation; Amino Acids; Animals; Blood Coagulation Disorders; Calcium; Carbohydrates; Cattle; Chemical Phenomena; Chemistry; Cyanogen Bromide; Dicumarol; Electrophoresis, Polyacrylamide Gel; Epitopes; Immunodiffusion; Isoelectric Focusing; Molecular Weight; Peptides; Protein Binding; Protein Conformation; Prothrombin; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Tyrosine; Ultracentrifugation; Vitamin K

Topics: Anticoagulants; Blood Coagulation Disorders; Blood Coagulation Factors; Blood Proteins; Calcium; Dicumarol; Fibrinolysis; Fibrinolytic Agents; Humans; Lipoproteins; Streptokinase; Thrombosis; Vitamin K

Topics: Blood Coagulation Disorders; Blood Transfusion; Dicumarol; Factor VII; Factor X; Factor XI; Hemophilia A; Humans; Liver Diseases; Male; Prothrombin; Vitamin K Deficiency; Vitamin K Deficiency Bleeding

Topics: Arteriovenous Fistula; Blood Coagulation Disorders; Dicumarol; Female; Hemodynamics; Humans; Hypoxia; Middle Aged; Pulmonary Circulation; Pulmonary Embolism

Topics: Acids; Adolescent; Adult; Anticoagulants; Blood Cell Count; Blood Coagulation Disorders; Blood Coagulation Tests; Blood Platelet Disorders; Blood Platelets; Child; Child, Preschool; Dicumarol; Drug Therapy; Edetic Acid; Female; Glucose; Hemophilia A; Heparin; Humans; Male; Middle Aged; Phosphates; Platelet Adhesiveness; Uremia; von Willebrand Diseases

Topics: Aged; Animals; Antidotes; Blood Coagulation Disorders; Child, Preschool; Chromatography; Coumarins; Dicumarol; Female; Humans; Male; Pedigree; Pharmacogenetics; Protein Binding; Prothrombin Time; Rabbits; Rats; Vitamin K; Vitamin K 1; Warfarin

Topics: Anticoagulants; Blood Coagulation Disorders; Dicumarol; Humans; Malingering

Topics: Animals; Blood Coagulation Disorders; Dicumarol; Female; Fetal Diseases; Humans; Kidney; Liver; Lung; Pregnancy; Pregnancy Complications, Cardiovascular; Rabbits; Thromboembolism

Topics: Aminocaproates; Aminocaproic Acid; Aprotinin; Blood Coagulation; Blood Coagulation Disorders; Blood Platelets; Deoxyribonuclease I; Dicumarol; Enzyme Inhibitors; Fibrinolysin; Fibrinolysis; Hemorrhagic Disorders; Heparin; Humans; Kallikreins; Physiology; Plasminogen; Prothrombin; Purpura; Streptodornase and Streptokinase; Streptokinase; Thrombin; Trypsin; Vitamin K; Waterhouse-Friderichsen Syndrome

Topics: Blood Coagulation Disorders; Blood Coagulation Factors; Blood Coagulation Tests; Dicumarol; Factor VII; Factor X; Humans; Myocardial Infarction; Pharmacology; Prothrombin; Research; Warfarin

Topics: Ascorbic Acid; Blood Coagulation Disorders; Blood Protein Disorders; Cold Temperature; Cryoglobulinemia; Cysteamine; Dicumarol; Fibrinogen; Humans; Penicillin G; Penicillins; Pyridoxine; Sulfhydryl Compounds; Telangiectasis

Topics: Blood Coagulation Disorders; Blood Coagulation Tests; Cholecystitis; Cholelithiasis; Dicumarol; Ethyl Biscoumacetate; Factor VII; Heparin; Humans; Pharmacology; Preoperative Care; Prothrombin

Topics: Biological Products; Blood Coagulation; Blood Coagulation Disorders; Dicumarol; Humans; Phospholipids

Topics: Anticoagulants; Blood Coagulation; Blood Coagulation Disorders; Dicumarol

Topics: Blood Coagulation Disorders; Blood Platelets; Coumarins; Dicumarol; Hemostatics; Prothrombin

Topics: Antifibrinolytic Agents; Blood; Blood Coagulation Disorders; Dicumarol; Heparin Antagonists; Humans; Vitamin K; Vitamin K Deficiency