dibekacin and Gram-Positive-Bacterial-Infections

Topics: Aminoglycosides; Animals; Anti-Bacterial Agents; Dibekacin; Drug Resistance, Microbial; Drug Therapy, Combination; Gram-Positive Bacterial Infections; Humans; Methicillin Resistance; Quinolones; Staphylococcal Infections; Staphylococcus aureus; Teicoplanin; Vancomycin

Enterococcus faecalis LC40 is an ampicillin-susceptible clinical isolate with high-level gentamicin resistance due to the aac(6')-Ie-aph(2")-Ia aminoglycoside resistance gene. The combination of ampicillin plus arbekacin reduced mean bacterial vegetation counts significantly more than ampicillin alone or ampicillin plus gentamicin in a rabbit model of aortic-valve endocarditis caused by E. faecalis LC40.

Topics: Aminoglycosides; Ampicillin; Animals; Anti-Bacterial Agents; Dibekacin; Disease Models, Animal; Drug Resistance, Microbial; Drug Therapy, Combination; Endocarditis, Bacterial; Enterococcus faecalis; Gentamicins; Gram-Positive Bacterial Infections; Microbial Sensitivity Tests; Rabbits; Treatment Outcome

Enterococci with gentamicin MICs of 256 to 1,024 micrograms/mL were evaluated for susceptibility to ampicillin plus gentamicin synergism. Sixteen of eighteen enterococcal isolates were not susceptible to synergistic killing by ampicillin plus gentamicin; 11 possessed aac(6')-aph(2"), and 4 possessed aph(2")-Ic. A gentamicin MIC of 512 or 1,024 micrograms/mL predicted lack of ampicillin/gentamicin synergism, but a gentamicin MIC of 256 micrograms/mL did not. For six enterococcal strains possessing the gentamicin-resistance gene aph(2")-Ic, ampicillin plus dibekacin, ampicillin plus netilmicin, and ampicillin plus amikacin produced synergistic killing in five, three, and two strains, respectively.

Topics: Amikacin; Ampicillin; Animals; Dibekacin; DNA Primers; DNA Probes; DNA Restriction Enzymes; Drug Resistance, Microbial; Drug Synergism; Electrophoresis, Agar Gel; Enterococcus faecium; Gentamicins; Gram-Positive Bacterial Infections; Humans; Microbial Sensitivity Tests; Netilmicin; Penicillins; Polymerase Chain Reaction

Topics: Aminoglycosides; Ampicillin; Anti-Bacterial Agents; Dibekacin; Drug Resistance, Microbial; Drug Therapy, Combination; Enterococcus faecalis; Enterococcus faecium; Gentamicins; Gram-Positive Bacterial Infections; Humans; Microbial Sensitivity Tests