dibekacin and Enterobacteriaceae-Infections

Topics: Clinical Trials as Topic; Dibekacin; Enterobacteriaceae Infections; Humans; Infant; Kanamycin; Staphylococcal Infections; Staphylococcus aureus

The in vivo efficacy of habekacin, an aminoglycoside antibiotic obtained by chemical derivation from dibekacin, was compared to that of gentamicin (GEN), tobramycin (TOB), kanamycin (KAN) and amikacin (AMI) in a protection test in mice. The 50% effective dose (ED50) was determined in groups of animals challenged with bacterial suspensions injected intraperitoneally together with mucin, and treated subcutaneously 1 h and 6 h later. The bacterial strains used were: Escherichia coli (three GEN sensitive strains and one GEN-KAN-TOB resistant strain), Enterobacter cloacae (one GEN-KAN resistant strain), Serratia marcescens and Klebsiella pneumoniae (one GEN sensitive strain and one GEN resistant strain). In this model, habekacin was found to be as active as GEN against GEN sensitive strains and more active than AMI on GEN, GEN-KAN and GEN-KAN-TOB resistant strains.

Topics: Amikacin; Aminoglycosides; Animals; Anti-Bacterial Agents; Dibekacin; Enterobacteriaceae Infections; Female; Gentamicins; Kanamycin; Male; Mice; Microbial Sensitivity Tests; Sepsis; Tobramycin

The combined actions of cefoxitin (CFX) with amikacin (AMK), gentamicin (GM) and dibekacin (DKB) were studied against Gram-positive and Gram-negative bacteria. The following results were obtained. The synergistic actions of CFX with AMK, GM and DKB were observed on S. aureus, S. epidermidis, E. coli, S. marcescens, K. pneumoniae, Proteus spp. and Acinetobacter by checker board titration method. The combination of CFX with AMK was most effective. In case of the combination of CFX with AMK, the simultaneous administration showed the highest bactericidal effect, followed by the case of addition of AMK after adding CFX. The phase-contrast microscopic observation on S. marcescens revealed that the bacterial cell prolonged with CFX showed a filament-like form and with AMK almost a normal form. In the combination, lysed cells were observed. The therapeutic experiment of S. marcescens infection in mice demonstrated that the combination of CFX with AMK showed superior effect than that of each drug alone.

Topics: Amikacin; Aminoglycosides; Animals; Bacteria; Cefoxitin; Dibekacin; Drug Evaluation, Preclinical; Drug Resistance, Microbial; Drug Synergism; Drug Therapy, Combination; Enterobacteriaceae Infections; Gentamicins; Kanamycin; Male; Mice